Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,240,984 (G)7→8 coding (1041/1245 nt) sstT → sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,240,9771.G94.6% 563.3 / 24.8 166coding (1034/1245 nt)sstTsodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base . (4/5);  new base G (84/73);  total (88/78)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

GGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATC  >  NC_000913/3240879‑3241071
                                                                                                    |                                                                                                 
  ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑ggg                                                                                              >  1:4243577/1‑100 (MQ=255)
  ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑ggg                                                                                              <  2:2239906/100‑1 (MQ=255)
    gCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑ggggg                                                                                            <  1:3090702/100‑1 (MQ=255)
    gCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑ggggg                                                                                            >  1:2812876/1‑100 (MQ=255)
       gTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑gggggggg                                                                                        <  1:3777922/100‑2 (MQ=255)
         tAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑ggggggggtc                                                                                      <  2:1700193/100‑4 (MQ=255)
          aaTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑ggggggggtct                                                                                     >  1:444565/1‑96 (MQ=255)
           aTACGCTTGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑ggggggggtctc                                                                                    <  1:4627987/100‑6 (MQ=255)
            tACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GGGGGAGGTctct                                                                                    <  2:1630598/100‑1 (MQ=255)
                 tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctgc                                                                               <  2:3785984/100‑1 (MQ=255)
                 tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGGTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctgc                                                                               >  2:5358058/1‑100 (MQ=255)
                  gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGCGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctgct                                                                              <  2:5001966/100‑1 (MQ=255)
                  gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctgct                                                                              <  1:333527/100‑1 (MQ=255)
                   ggTATTGCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctgctg                                                                             <  2:2984836/100‑1 (MQ=255)
                      aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATc                                                                          <  1:175568/100‑1 (MQ=255)
                      aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATc                                                                          <  2:494455/100‑1 (MQ=255)
                      aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATc                                                                          >  2:266962/1‑100 (MQ=255)
                      aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATc                                                                          >  1:4764630/1‑100 (MQ=255)
                       ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATcc                                                                         >  1:5128718/1‑100 (MQ=255)
                        tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATccc                                                                        <  1:5408985/100‑1 (MQ=255)
                        tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATccc                                                                        <  2:296919/100‑1 (MQ=255)
                        tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATccc                                                                        <  2:5221767/100‑1 (MQ=255)
                         ccGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                       >  1:1601612/1‑100 (MQ=255)
                         ccGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                       >  1:1375556/1‑100 (MQ=255)
                         ccGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                       >  2:4119997/1‑100 (MQ=255)
                           tgTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                     <  2:4819199/99‑1 (MQ=255)
                           ggTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                     <  1:2504865/100‑1 (MQ=255)
                               taTCTCCCCACGGTGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTACCTGTGGCGCATCCGGCGTGGC‑‑GGGGGCGGTCTCTGCTGTTGAGCCCACTGGcc                                                                 <  2:5126451/99‑1 (MQ=255)
                               gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGcc                                                                 <  1:1587861/100‑1 (MQ=255)
                               gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGcc                                                                 <  1:1799614/100‑1 (MQ=255)
                                aTCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCt                                                                >  1:4291532/1‑100 (MQ=255)
                                aTCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCATGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCt                                                                >  1:4187896/1‑100 (MQ=255)
                                aTCCGCCCACGGCGCTGGTGTTGGGGGTGGGGGCTTCTCTGTGTGCCTGTGGCGCATCCGGGGTGGCGGGGGGGGGTCTCTGCTGCTGATCCCACTGGcc                                                                 <  1:2985457/100‑1 (MQ=255)
                                  cTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGt                                                              >  2:4141494/1‑100 (MQ=255)
                                   tGCCCACGGCGCTGCTGTTGGGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                             <  1:1541526/100‑1 (MQ=255)
                                   tGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                             <  1:561125/100‑1 (MQ=255)
                                       cACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAatat                                                         >  1:22214/1‑100 (MQ=255)
                                         cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGt                                                       >  2:1475039/1‑100 (MQ=255)
                                           gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                     <  1:2279085/100‑1 (MQ=255)
                                              ctgctgTTGAGCGTGGTGGCTTCTCTGTGTTCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCGCTGGCCTGTTATATGTTCggc                                                  >  1:2190684/1‑99 (MQ=255)
                                                gctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCAGCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTAt                                                >  1:4823272/1‑100 (MQ=255)
                                                  tgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGCTCGGTAttt                                              <  1:5431037/100‑1 (MQ=255)
                                                   gTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTc                                             <  1:2438241/100‑1 (MQ=255)
                                                   gTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTc                                             <  1:2005263/100‑1 (MQ=255)
                                                   gTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTc                                             <  2:488403/100‑1 (MQ=255)
                                                       aGCGTGGTGGCTGCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGCCACCACTGGCCTGTAATATGTTCGGTATTTCGaag                                         <  2:2390508/100‑2 (MQ=255)
                                                        gCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACg                                        >  2:1975311/1‑100 (MQ=255)
                                                          gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGat                                      >  1:744229/1‑100 (MQ=255)
                                                              tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGGCTGTAATATGTTCGGTATTTCTAACGATATCg                                  >  1:4726475/1‑100 (MQ=255)
                                                              tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCg                                  >  2:1792890/1‑100 (MQ=255)
                                                               ggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGc                                 >  1:4570347/1‑100 (MQ=255)
                                                               ggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGGGGC‑‑GGAGGGGGTCACTGCTGCTGATCCCACTGGCCTGTAATATGTTCCGTATTTCGAACGATATCGc                                 >  1:4953732/1‑100 (MQ=255)
                                                                 cTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTTTTTCGAACGATATTGCCa                               >  1:3554746/1‑100 (MQ=255)
                                                                 cTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCa                               <  2:1314867/100‑1 (MQ=255)
                                                                 cTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCa                               >  2:1469716/1‑100 (MQ=255)
                                                                 cTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCa                               >  2:3172824/1‑100 (MQ=255)
                                                                     tctGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCa                           <  1:4538260/100‑1 (MQ=255)
                                                                      ctGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                          >  2:3998003/1‑100 (MQ=255)
                                                                      ctGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                          >  2:2002787/1‑100 (MQ=255)
                                                                       tgtgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAgg                         <  2:423194/100‑1 (MQ=255)
                                                                        gtgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggt                        >  1:5155356/1‑100 (MQ=255)
                                                                        ctgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTATGCTGTTGGTCCCACTGGCCTGTAATATGTTCGGTAGTTGGAACGATATCGCCATGCGggt                        >  2:2787677/2‑100 (MQ=255)
                                                                         tgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtg                       <  1:2866808/100‑1 (MQ=255)
                                                                          gtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtgg                      >  1:1247446/1‑100 (MQ=255)
                                                                           tgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtggt                     <  1:4967713/100‑1 (MQ=255)
                                                                           tgCCTGTGGCGCATCCGGCGTGGC‑‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGtt                    >  2:3354429/1‑100 (MQ=255)
                                                                           ggCCTGTGGCGCATCCGGCGGGGGG‑‑GGGGGGGTCTCTGCTGCTGATCCCATTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtggt                     <  2:825372/99‑1 (MQ=255)
                                                                            gCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGtt                    >  1:1560177/1‑100 (MQ=255)
                                                                             ccTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTg                   <  1:2068675/100‑1 (MQ=255)
                                                                              cTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTTGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGc                  >  1:831920/1‑100 (MQ=255)
                                                                              cTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCCTGCAGGTGGTTGc                  >  2:1186054/1‑100 (MQ=255)
                                                                              cTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGc                  >  2:1183657/1‑100 (MQ=255)
                                                                              cTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGc                  >  1:2493690/1‑100 (MQ=255)
                                                                              cTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGc                  >  2:3495524/1‑100 (MQ=255)
                                                                               tgtgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGcc                 <  1:169843/100‑1 (MQ=255)
                                                                               tgtgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGcc                 >  1:2684633/1‑100 (MQ=255)
                                                                               tgtgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATACGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGcc                 >  1:4918121/1‑100 (MQ=255)
                                                                                gtgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                <  2:3302135/100‑1 (MQ=255)
                                                                                gtgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                >  2:4875562/1‑100 (MQ=255)
                                                                                  ggCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATCTATTCGGTATTTCGAACGATCTCGCCATGCAGGCCGTTGCCGTc              >  1:4853421/1‑100 (MQ=255)
                                                                                  ggCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTc              >  1:3457186/1‑100 (MQ=255)
                                                                                  ggCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTc              >  2:2279626/1‑100 (MQ=255)
                                                                                  ggCGCATCCGGCGTGGCGGGGGGGGTCGCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTc              <  1:1494074/100‑1 (MQ=255)
                                                                                   gcgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCg             <  2:655170/100‑1 (MQ=255)
                                                                                   gcgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCg             >  2:4297449/1‑100 (MQ=255)
                                                                                    cgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCgg            <  1:1142262/100‑1 (MQ=255)
                                                                                    cgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCgg            <  1:3712088/100‑1 (MQ=255)
                                                                                    cgcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCgg            <  1:4378822/100‑1 (MQ=255)
                                                                                     gcATCCGGGGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGc           <  2:2020442/100‑1 (MQ=255)
                                                                                     gcATCCGGCGGGTCGGGGGGGGCCTCTGGTGCTGAACCCACTGGCCGGTAATATGTTCGGTATTTCCAACGATGGCGCCGGGGAGGTGGTTGCCATCGGc           >  2:3642060/1‑100 (MQ=255)
                                                                                      cATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCt          >  2:420062/1‑100 (MQ=255)
                                                                                       aTCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCtt         <  1:1476288/100‑1 (MQ=255)
                                                                                        tCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCttt        >  2:2939077/1‑100 (MQ=255)
                                                                                         ccGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTa       >  1:3651039/1‑100 (MQ=255)
                                                                                          cgtcGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTTCCGTCGGCTTTat      >  2:2493203/4‑100 (MQ=255)
                                                                                           ggCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatc     >  1:3950094/1‑100 (MQ=255)
                                                                                           ggCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatc     <  2:58034/100‑1 (MQ=255)
                                                                                             cGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat   <  2:442031/100‑1 (MQ=255)
                                                                                               gTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCCTGCAGGTGGTTGCCGTCGGCCTTatcctc  >  2:3061374/1‑100 (MQ=255)
                                                                                               gTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcatc  <  2:5084504/100‑1 (MQ=255)
                                                                                                    |                                                                                                 
GGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATC  >  NC_000913/3240879‑3241071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: