Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 750,404 +C coding (1766/2448 nt) ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913750,4001.C96.1% 617.3 / 16.0 178coding (1770/2448 nt)ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (3/4);  new base C (88/83);  total (91/87)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

ACCAGAAATATCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAACCCGACCAC  >  NC_000913/750301‑750494
                                                                                                    |                                                                                                
aCCAGAAATATCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATg                                                                                                   >  1:3839949/1‑100 (MQ=255)
 ccAGAAATATCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGc                                                                                                 >  1:2818265/1‑100 (MQ=255)
  cAGAAATATCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGcc                                                                                               >  1:2153467/1‑100 (MQ=255)
   aGAAATATCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGccc                                                                                              <  1:1695929/100‑1 (MQ=255)
   aGAAATATCCCACTCACACGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGCCAGACATGcca                                                                                              >  2:2978997/1‑99 (MQ=255)
    taaaTATCCCGCTCACCCGCTGAACTCAGGTGGTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGTTGGGATTGTAGTCCGGCAGACATGcccc                                                                                            <  2:4284185/99‑1 (MQ=255)
     aaaTATCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGccccc                                                                                           >  1:4493391/1‑99 (MQ=255)
     aaaTATCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGccccc                                                                                           <  2:3336892/100‑2 (MQ=255)
     aaaTATCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCa                                                                                           <  1:1044183/100‑1 (MQ=255)
      aaTATCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGccccca                                                                                          >  1:2951899/1‑98 (MQ=255)
       atatCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGcccccag                                                                                         >  2:2598794/1‑97 (MQ=255)
              aCTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTAc                                                                                   <  1:1757464/100‑1 (MQ=255)
              aCTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTAc                                                                                   <  1:3944716/100‑1 (MQ=255)
               cTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACg                                                                                  >  2:1766611/1‑100 (MQ=255)
               cTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACg                                                                                  >  1:3667477/1‑100 (MQ=255)
                 cACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTGCGtt                                                                                >  2:1379180/1‑100 (MQ=255)
                 cACCCGCTGAACTCAGGTGCTGATAGGTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGCATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGtt                                                                                <  2:5373291/100‑1 (MQ=255)
                  aCCCGCTGAACTCAGGTGCGGGTAGTTACCGCTCACCTGGGCGCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTGCGtta                                                                               >  1:4749764/1‑99 (MQ=255)
                    ccGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCAt                                                                             <  2:4592490/100‑1 (MQ=255)
                     cGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATc                                                                            <  1:1397802/100‑1 (MQ=255)
                     cGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATc                                                                            >  2:1889896/1‑100 (MQ=255)
                       cTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCa                                                                          >  2:3509184/1‑100 (MQ=255)
                          aaCTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCg                                                                       <  1:2524907/100‑1 (MQ=255)
                           aCTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGAAATGCCCCCAGCTATTACGTTCATCCAGCGt                                                                      >  1:2443706/1‑100 (MQ=255)
                            cTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTa                                                                     >  2:4935460/1‑100 (MQ=255)
                             tCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTAt                                                                    <  1:2013585/100‑1 (MQ=255)
                                ggTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGt                                                                 >  2:4722314/1‑100 (MQ=255)
                                ggTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGt                                                                 >  2:1362401/1‑100 (MQ=255)
                                 gTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGtt                                                                <  1:1954822/100‑1 (MQ=255)
                                   gCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTcc                                                              >  2:5344118/1‑100 (MQ=255)
                                    cTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCa                                                             <  2:1934534/100‑1 (MQ=255)
                                    cTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCa                                                             >  1:4095589/1‑100 (MQ=255)
                                    cTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCAc                                                            <  1:32628/100‑1 (MQ=255)
                                     tGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCAc                                                            >  1:1605463/1‑100 (MQ=255)
                                     tGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCAc                                                            <  1:1857045/100‑1 (MQ=255)
                                     tGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCAc                                                            >  2:4681612/1‑100 (MQ=255)
                                     tGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACg                                                           >  1:1424199/1‑100 (MQ=255)
                                        tAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGAc                                                         <  1:1786978/100‑1 (MQ=255)
                                         aGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACa                                                        >  1:967421/1‑100 (MQ=255)
                                          gTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACAt                                                       <  2:4536301/100‑1 (MQ=255)
                                          gTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACAt                                                       >  1:4226362/1‑100 (MQ=255)
                                            tACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGc                                                     <  1:3376267/100‑1 (MQ=255)
                                            tACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGc                                                     >  1:3326414/1‑100 (MQ=255)
                                            tACCGCACACCTGGGCTCCATTGTCCGGACGATCGGATTTCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGc                                                     >  2:5071278/1‑100 (MQ=255)
                                            naCCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTCTTACGTTCATCCAGCGTATCGTTCCACGACATGc                                                     >  1:1907508/2‑100 (MQ=255)
                                             aCCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCg                                                    >  1:1654517/1‑100 (MQ=255)
                                              ccGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCgg                                                   >  2:2724230/1‑100 (MQ=255)
                                              ccGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCgg                                                   >  1:4385811/1‑100 (MQ=255)
                                              ccGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCgg                                                   <  2:1177885/100‑1 (MQ=255)
                                               cGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGgt                                                  <  2:4866534/100‑1 (MQ=255)
                                                gCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGgtg                                                 <  2:2084906/100‑1 (MQ=255)
                                                gCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGgtg                                                 <  2:5483351/100‑1 (MQ=255)
                                                 cTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGgtgt                                                >  2:273222/1‑100 (MQ=255)
                                                   cACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTAt                                              >  1:4301619/1‑100 (MQ=255)
                                                    aCCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATg                                             <  2:4087583/100‑1 (MQ=255)
                                                     ccTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATgg                                            <  2:2272216/100‑1 (MQ=255)
                                                     ccTGGGCTCCATTGGCCGGACGATCGGAGTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATgg                                            <  2:2473273/100‑1 (MQ=255)
                                                      cTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGt                                           >  2:2009639/1‑100 (MQ=255)
                                                           cTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCtgt                                      >  1:3953532/1‑100 (MQ=255)
                                                            tCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCtgtg                                     <  1:5417003/100‑1 (MQ=255)
                                                              cATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGc                                   >  2:1242674/1‑100 (MQ=255)
                                                              cATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGc                                   <  2:1107025/100‑1 (MQ=255)
                                                              cATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTACTGTGGCt                                  <  2:5268035/100‑1 (MQ=255)
                                                                  tctcGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACt                               <  1:123618/97‑1 (MQ=255)
                                                                  tctcGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACt                               <  1:2922559/97‑1 (MQ=255)
                                                                  gTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACt                               >  2:2164283/1‑100 (MQ=255)
                                                                  gTCCGGACGATCGAATTGCAGTCCGGCACACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTCCTGTGGCTACt                               >  1:4903639/1‑100 (MQ=255)
                                                                    ccGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGt                             >  2:3932810/1‑100 (MQ=255)
                                                                    ccGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGt                             >  1:1430591/1‑100 (MQ=255)
                                                                    ccGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGt                             <  1:2683562/100‑1 (MQ=255)
                                                                     cGGACGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACtaga                            >  2:2895006/1‑97 (MQ=255)
                                                                         cGATCGGATTGTAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGc                        >  2:1075901/1‑100 (MQ=255)
                                                                         cGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGc                        >  1:1356024/1‑100 (MQ=255)
                                                                         cGATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGc                        <  2:3804759/100‑1 (MQ=255)
                                                                         cGATCGGATTGCAGGCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGc                        <  1:681983/100‑1 (MQ=255)
                                                                          gATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCa                       >  1:4622680/1‑100 (MQ=255)
                                                                          gATCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCa                       <  1:406052/100‑1 (MQ=255)
                                                                           aTCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCAt                      >  1:3100010/1‑100 (MQ=255)
                                                                           aTCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCAt                      <  1:2256625/100‑1 (MQ=255)
                                                                           aTCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCAt                      <  2:4198996/100‑1 (MQ=255)
                                                                           aTCGGATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCAt                      <  1:3977583/100‑1 (MQ=255)
                                                                              ggATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTc                   <  2:1703551/100‑1 (MQ=255)
                                                                               gATTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGGGCTGTGGCTACTGTTTTGCATGTCa                  <  2:3437057/100‑1 (MQ=255)
                                                                                aTTGCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTGCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCAt                 <  1:2903007/100‑1 (MQ=255)
                                                                                   gCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGc              <  2:4014529/100‑1 (MQ=255)
                                                                                   gCAGTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGc              <  1:4480413/100‑1 (MQ=255)
                                                                                    gactCCGGCAGACCTTCTCCCAGCTATTACGTTCATCCAGCGCCTCGTCCCCCGACATGCGGTGTATGGTGCCGTGTCTACTGTTTTGCATGTCATAGcc             >  2:3271286/4‑100 (MQ=255)
                                                                                      gTCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCaa           >  1:794008/1‑100 (MQ=255)
                                                                                       tCCGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAAc          <  1:762924/100‑1 (MQ=255)
                                                                                        ccGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCaatc         >  2:1450528/1‑98 (MQ=255)
                                                                                         tggCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAAccc        <  1:4672537/99‑1 (MQ=255)
                                                                                         cGGCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAAccc        >  1:1539610/1‑100 (MQ=255)
                                                                                          ggCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAAccca       >  2:4615579/1‑99 (MQ=255)
                                                                                          ggCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAACCCg       >  2:4437282/1‑100 (MQ=255)
                                                                                          ggCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAACCCg       >  1:3519805/1‑100 (MQ=255)
                                                                                          ggCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAACCCg       >  1:634695/1‑100 (MQ=255)
                                                                                          ggCAGACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAACCCg       >  1:1257707/1‑100 (MQ=255)
                                                                                              gACATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAACCCGacca   >  1:2979021/1‑100 (MQ=255)
                                                                                               aCATGCCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAACCCGaccac  <  1:275869/100‑1 (MQ=255)
                                                                                               aCATGCCCACAGGTATTACGTTCATCCAGCGTATCGTTTCCCGACATGGGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAACCCGaccac  <  2:4755492/100‑1 (MQ=255)
                                                                                                    |                                                                                                
ACCAGAAATATCCCACTCACCCGCTGAACTCAGGTGCTGATAGTTACCGCTCACCTGGGCTCCATTGTCCGGACGATCGGATTGCAGTCCGGCAGACATGCCCCAGCTATTACGTTCATCCAGCGTATCGTTCCACGACATGCGGTGTATGGTGCTGTGGCTACTGTTTTGCATGTCATAGCCAACCCGACCAC  >  NC_000913/750301‑750494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: