Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 299,214 A→C F574C (TTC→TGC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913299,2140AC98.0% 697.9 / 0.6 197F574C (TTC→TGC) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base C (113/80);  total (114/83)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

ACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACT  >  NC_000913/299117‑299310
                                                                                                 |                                                                                                
 cGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTCCTCTTTGCTCAGTGTTCCGCAtt                                                                                               >  2:1582475/1‑100 (MQ=255)
  gCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTc                                                                                              >  1:3377879/1‑100 (MQ=255)
   ccGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCa                                                                                             <  1:3722879/100‑1 (MQ=255)
   ccGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCa                                                                                             <  2:2934565/100‑1 (MQ=255)
     ggACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAAt                                                                                           <  2:3939980/100‑1 (MQ=255)
       gcccGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACACAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGc                                                                                         <  1:4609253/99‑1 (MQ=255)
       aCCCGCACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGc                                                                                         <  2:2465812/100‑1 (MQ=255)
       aCCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGc                                                                                         <  1:5497560/100‑1 (MQ=255)
       aCCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGc                                                                                         <  1:3213961/100‑1 (MQ=255)
        cccGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGct                                                                                        >  2:876259/1‑100 (MQ=255)
        cccGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGct                                                                                        >  1:5061615/1‑100 (MQ=255)
         ccGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGctc                                                                                       >  1:2105132/1‑100 (MQ=255)
          cGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGctct                                                                                      >  1:3065643/1‑100 (MQ=255)
               ttCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTcc                                                                                 >  2:4362387/1‑100 (MQ=255)
               ttCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTcc                                                                                 <  2:1763746/100‑1 (MQ=255)
                tctcCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCg                                                                                <  1:2841487/100‑1 (MQ=255)
                 ctcCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCga                                                                               >  2:2814437/1‑99 (MQ=255)
                 ctcCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGc                                                                               <  1:4967114/100‑1 (MQ=255)
                 ctcCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGc                                                                               <  1:4074437/100‑1 (MQ=255)
                   cccGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCgaga                                                                             >  1:1624681/1‑97 (MQ=255)
                   cccGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTg                                                                             <  2:3553351/100‑1 (MQ=255)
                     cGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGTCAAAGGTCGACTCCTGCTACTCTTTGCTCATTGTTTCGCATTCAATGCTCGCTTCCGCTGTc                                                                           >  2:1090375/1‑100 (MQ=255)
                     cGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTc                                                                           <  2:596316/100‑1 (MQ=255)
                     cGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTc                                                                           <  2:170373/100‑1 (MQ=255)
                       tCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAg                                                                         <  1:5567251/100‑1 (MQ=255)
                        cgcgCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGt                                                                        >  1:1845500/1‑100 (MQ=255)
                           gcTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTg                                                                     <  2:2917738/100‑1 (MQ=255)
                           gcTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTg                                                                     >  1:2674510/1‑100 (MQ=255)
                               tGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTgccgc                                                                 >  2:2226228/1‑100 (MQ=255)
                                gCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTgccgcc                                                                >  2:4844028/1‑100 (MQ=255)
                                gCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTgccgcc                                                                <  2:3844054/100‑1 (MQ=255)
                                 cACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCt                                                               >  2:2650743/1‑100 (MQ=255)
                                   cGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTgn                                                             <  1:1497140/100‑2 (MQ=255)
                                        aCCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTgg                                                        >  1:514696/1‑100 (MQ=255)
                                         ccTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGc                                                       >  2:1190208/1‑100 (MQ=255)
                                         ccTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGc                                                       <  1:2249357/100‑1 (MQ=255)
                                          cTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCt                                                      <  1:314619/100‑1 (MQ=255)
                                          cTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCt                                                      <  1:4565627/100‑1 (MQ=255)
                                           tCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCtt                                                     >  1:48833/1‑100 (MQ=255)
                                           tCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCtt                                                     >  1:2652883/1‑100 (MQ=255)
                                            ccACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTc                                                    >  2:3086874/1‑100 (MQ=255)
                                            ccACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTc                                                    >  1:1121367/1‑100 (MQ=255)
                                            ccACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTc                                                    >  1:2977278/1‑100 (MQ=255)
                                             cacaAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCa                                                   <  2:1483590/100‑1 (MQ=255)
                                             cacaAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCa                                                   <  1:2529323/100‑1 (MQ=255)
                                             cacaAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCa                                                   <  2:2072025/100‑1 (MQ=255)
                                             cacaAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCa                                                   <  1:278940/100‑1 (MQ=255)
                                              acaAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCAt                                                  >  1:494240/1‑100 (MQ=255)
                                              acaAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCAt                                                  <  1:4163289/100‑1 (MQ=255)
                                                aaaaTGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGGTCAGTGTTACGCATTCAATGCTCTCTTACGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGt                                                <  1:5180484/100‑1 (MQ=255)
                                                  aaTGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAg                                              <  1:1320095/100‑1 (MQ=255)
                                                     gCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGt                                           >  1:3189573/1‑100 (MQ=255)
                                                       ccGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCCTGTAGCGTgg                                         >  2:3010163/1‑100 (MQ=255)
                                                       ccGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTgg                                         <  2:428455/100‑1 (MQ=255)
                                                        cGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgc                                        <  2:48311/100‑1 (MQ=255)
                                                        cGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgc                                        <  2:3144778/100‑1 (MQ=255)
                                                         ggCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgcg                                       >  1:2433334/1‑100 (MQ=255)
                                                         ggCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgcg                                       <  2:3414950/100‑1 (MQ=255)
                                                          gCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGgcgc                                      >  1:1214552/1‑100 (MQ=255)
                                                           cAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCt                                     >  1:3294833/1‑100 (MQ=255)
                                                            aaaGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCtt                                    >  1:3236002/1‑100 (MQ=255)
                                                            aaaGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCtt                                    >  2:504999/1‑100 (MQ=255)
                                                             tctgTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTc                                   <  1:5272815/97‑1 (MQ=255)
                                                              aGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCg                                  <  1:238938/100‑1 (MQ=255)
                                                              aGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCg                                  <  1:520981/100‑1 (MQ=255)
                                                               ggTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCgg                                 <  1:3509990/100‑1 (MQ=255)
                                                               ggTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCgg                                 <  2:4722619/100‑1 (MQ=255)
                                                                gTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCggg                                <  1:6479/100‑1 (MQ=255)
                                                                gTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCggg                                >  1:4801794/1‑100 (MQ=255)
                                                                 tCGACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGt                               <  1:1044501/100‑1 (MQ=255)
                                                                   gACTGCTGGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTAc                             >  2:2017659/1‑100 (MQ=255)
                                                                     ctgctgGTACTCTTTGCTCAGTGTTCCGCATTCCATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCg                           <  2:1435745/100‑1 (MQ=255)
                                                                       gctgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGtt                         >  1:3737316/1‑100 (MQ=255)
                                                                       gctgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGtt                         >  1:191970/1‑100 (MQ=255)
                                                                        ctgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTg                        >  2:4823844/1‑100 (MQ=255)
                                                                         tgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTgg                       <  1:2711743/100‑1 (MQ=255)
                                                                         tgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTgg                       <  2:1802015/100‑1 (MQ=255)
                                                                         tgGTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTgg                       <  2:3346497/100‑1 (MQ=255)
                                                                          ggTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGt                      >  2:2311515/1‑100 (MQ=255)
                                                                          ggTACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGt                      <  2:3681985/100‑1 (MQ=255)
                                                                            tACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTaa                    >  1:2912115/1‑100 (MQ=255)
                                                                            tACTCTTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTaa                    >  1:355983/1‑100 (MQ=255)
                                                                              ctctTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATc                  >  1:847864/1‑100 (MQ=255)
                                                                              ctctTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATc                  >  2:1632681/1‑100 (MQ=255)
                                                                               tctTTGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCt                 <  2:3833703/100‑1 (MQ=255)
                                                                                 tttGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTg               <  2:2728119/100‑1 (MQ=255)
                                                                                   tGCTCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGcg             <  1:2039509/100‑2 (MQ=255)
                                                                                    gCTCGGTGTTCCGCCTTCCCTGCTCTCTTACGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCg            <  2:251867/100‑1 (MQ=255)
                                                                                      tCAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTc          <  1:2649819/100‑1 (MQ=255)
                                                                                       cAGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCg         >  1:3948873/1‑100 (MQ=255)
                                                                                        aGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCgg        <  2:4043802/100‑1 (MQ=255)
                                                                                        aGTGTTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCgg        <  1:4933278/100‑1 (MQ=255)
                                                                                         gtgtTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCTGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGc       >  2:5115879/1‑100 (MQ=255)
                                                                                         gtgtTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGc       >  2:201957/1‑100 (MQ=255)
                                                                                         gtgtTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGc       >  1:3727444/1‑100 (MQ=255)
                                                                                         gtgtTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGc       >  2:659521/1‑100 (MQ=255)
                                                                                           gtTCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCaa     >  1:2008051/1‑100 (MQ=255)
                                                                                             tCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAAc   >  1:1903741/1‑100 (MQ=255)
                                                                                             tCCGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAAc   <  1:763987/100‑1 (MQ=255)
                                                                                              ccGCATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGTGTGGCGCTTCGGGTACGGTTGGTAATCTTGCCGTCGGCAACCt  >  2:1103011/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ACGCCGGACCCGAACTTCTCCCGTCGCGCTATGCACGCCGACCTCCACAAAATGCCCGGCAAAGGTCGACTGCTGGTACTCTTTGCTCAGTGTTCCGAATTCAATGCTCTCTTCCGCTGTCAGTCTGCCGCCTGCGGTGGCTTCATGTAGCGTGGCGCTTCGGGTACCGTTGGTAATCTTGCCGTCGGCAAACT  >  NC_000913/299117‑299310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: