Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 313,275 T→G intergenic (‑33/+441) ykgR ← / ← ykgP uncharacterized protein/pseudogene, oxidoreductase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913313,2750TG99.2% 885.6 / ‑2.4 248intergenic (‑33/+441)ykgR/ykgPuncharacterized protein/pseudogene, oxidoreductase family
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (100/145);  total (101/147)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 248 total aligned reads displayed.

ATTGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATT  >  NC_000913/313178‑313371
                                                                                                 |                                                                                                
aTTGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCg                                                                                                <  1:2057452/100‑1 (MQ=255)
 ttGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGt                                                                                               <  2:1608145/100‑1 (MQ=255)
   gCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTaa                                                                                             >  2:2137792/1‑100 (MQ=255)
    cGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAg                                                                                            >  2:4084194/1‑100 (MQ=255)
     gACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGt                                                                                           >  2:2976156/1‑100 (MQ=255)
       cAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGc                                                                                         >  1:269178/1‑100 (MQ=255)
       cAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGc                                                                                         >  2:4924537/1‑100 (MQ=255)
       cAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGc                                                                                         >  1:1877895/1‑100 (MQ=255)
            acaacGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTta                                                                                    <  1:2923855/100‑1 (MQ=255)
            acaacGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTta                                                                                    <  2:4781290/100‑1 (MQ=255)
             caacGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTtat                                                                                   <  1:92403/100‑1 (MQ=255)
             caacGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTtat                                                                                   <  2:2094201/100‑1 (MQ=255)
             caacGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTtat                                                                                   >  1:2141806/1‑100 (MQ=255)
             caacGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTtat                                                                                   >  2:5446696/1‑100 (MQ=255)
               acGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTtatat                                                                                 >  2:1390047/1‑100 (MQ=255)
               acGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTtatat                                                                                 <  2:623639/100‑1 (MQ=255)
                cGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTtatata                                                                                >  1:3990970/1‑100 (MQ=255)
                  atatTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATataaa                                                                              >  1:1437590/1‑100 (MQ=255)
                   tatTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATataaat                                                                             <  2:2256510/100‑1 (MQ=255)
                    atTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAACAGTGCACTGCGTAAGTGCCCTTATataaata                                                                            <  2:3723340/100‑1 (MQ=255)
                     ttAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATataaataa                                                                           >  1:3550755/1‑100 (MQ=255)
                       aaTCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACg                                                                         >  2:2823136/1‑100 (MQ=255)
                       aaTCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACg                                                                         <  1:793398/100‑1 (MQ=255)
                       aaTCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACg                                                                         >  2:1009035/1‑100 (MQ=255)
                       aaTCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACg                                                                         <  1:3789204/100‑1 (MQ=255)
                        aTCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGa                                                                        >  2:123325/1‑100 (MQ=255)
                        aTCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGa                                                                        >  1:4560057/1‑100 (MQ=255)
                         tCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAg                                                                       <  1:5418780/100‑1 (MQ=255)
                         tCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAg                                                                       <  1:1033062/100‑1 (MQ=255)
                            gTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATACATAACGAGttt                                                                    >  2:4391320/1‑100 (MQ=255)
                             tttATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTg                                                                   <  2:4136412/100‑1 (MQ=255)
                              ttATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTgg                                                                  <  1:4813996/100‑1 (MQ=255)
                               tATGACTGATTTGCTGTACTTTATTCTCTTTGATTGGTACTTCCTCGCTTTAAAAAAGAGTGGAATGCGTAAGTGCCCTTATATAAATAACGCTTTTgat                                                                 >  1:4467305/1‑98 (MQ=255)
                                aTGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTc                                                                <  1:3589581/100‑1 (MQ=255)
                                 tgaaggATTGGCTGTACTTGATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCATTATATAAATAACGAGTTTGGTCa                                                               <  2:4222769/95‑1 (MQ=255)
                                  gACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCaa                                                              >  2:845625/1‑100 (MQ=255)
                                      gATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCaa                                                          >  1:3076911/1‑100 (MQ=255)
                                       aTTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAt                                                         >  1:5292075/1‑100 (MQ=255)
                                       aTTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAt                                                         >  2:700981/1‑100 (MQ=255)
                                           gCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAttttt                                                     >  2:2281418/1‑100 (MQ=255)
                                           gCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAttttt                                                     >  2:2562050/1‑100 (MQ=255)
                                           gCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAttttt                                                     <  2:878541/100‑1 (MQ=255)
                                           gCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACCGCGTAAGTGCGCTTATATAAATAACGAGTTTGGTCAACCAAttttt                                                     <  1:5376527/100‑1 (MQ=255)
                                            cTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAtttttt                                                    >  1:4934350/1‑100 (MQ=255)
                                            cTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAAtttttt                                                    <  2:4357321/100‑1 (MQ=255)
                                             tGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGTGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTg                                                   <  2:2208951/100‑1 (MQ=255)
                                             tGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTg                                                   <  2:449696/100‑1 (MQ=255)
                                              gTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGa                                                  >  2:3359730/1‑100 (MQ=255)
                                               tACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAATAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGAc                                                 <  2:5005240/100‑1 (MQ=255)
                                               tACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGAc                                                 <  2:3709641/100‑1 (MQ=255)
                                               tACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGAc                                                 >  1:2398692/1‑100 (MQ=255)
                                                ncTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACa                                                >  2:5276322/2‑100 (MQ=255)
                                                aCTTTATTGTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCACTTATATAAATAGCGAGTTTGGTCAACCAATTTTTTGACa                                                >  2:5261225/1‑100 (MQ=255)
                                                aCTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACa                                                <  1:2132610/100‑1 (MQ=255)
                                                aCTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACa                                                <  1:2587431/100‑1 (MQ=255)
                                                aCTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACa                                                >  1:208278/1‑100 (MQ=255)
                                                  tttATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATg                                              <  1:1644250/100‑1 (MQ=255)
                                                    tATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTa                                            <  2:3546823/100‑1 (MQ=255)
                                                    tATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTa                                            <  2:4709240/100‑1 (MQ=255)
                                                    tATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGAAATGTa                                            >  1:4629554/1‑100 (MQ=255)
                                                     aTTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTAt                                           <  1:3004263/100‑1 (MQ=255)
                                                     aTTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTAt                                           <  2:3629150/100‑1 (MQ=255)
                                                       tctcTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATca                                         <  2:4526533/100‑1 (MQ=255)
                                                       tctcTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATca                                         >  2:4024406/1‑100 (MQ=255)
                                                         tctTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATcaca                                       >  2:3211675/1‑100 (MQ=255)
                                                              cATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAAttt                                  <  2:3037633/100‑1 (MQ=255)
                                                              cATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAAttt                                  >  1:2565772/1‑100 (MQ=255)
                                                                 tGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGaa                               >  1:3910552/1‑100 (MQ=255)
                                                                 tGGTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGaa                               <  1:4691842/100‑1 (MQ=255)
                                                                  ggTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGaan                              <  1:4424216/100‑2 (MQ=255)
                                                                  ggTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAAt                              >  1:1136622/1‑100 (MQ=255)
                                                                  ggTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAAt                              <  2:3466358/100‑1 (MQ=255)
                                                                   gTACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATa                             <  2:1777696/100‑1 (MQ=255)
                                                                    tACTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAg                            >  1:4183326/1‑100 (MQ=255)
                                                                      cTTCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGAt                          >  1:5559540/1‑100 (MQ=255)
                                                                       ttCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATg                         <  1:3831944/100‑1 (MQ=255)
                                                                        tCCTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGt                        <  2:4986091/100‑1 (MQ=255)
                                                                         ccTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTa                       >  1:27544/1‑100 (MQ=255)
                                                                         ccTCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTa                       <  1:1788690/100‑1 (MQ=255)
                                                                           tCGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTAtt                     <  1:5531459/100‑1 (MQ=255)
                                                                            cGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTa                    <  1:818139/100‑1 (MQ=255)
                                                                            cGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTa                    <  2:1819529/100‑1 (MQ=255)
                                                                            cGCTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTa                    <  2:2503518/100‑1 (MQ=255)
                                                                              cTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACa                  >  1:4089410/1‑100 (MQ=255)
                                                                              cTTTAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACa                  >  2:1883162/1‑100 (MQ=255)
                                                                               tttAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACAt                 >  2:2688134/1‑100 (MQ=255)
                                                                                ttAAAAAAGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATc                >  1:2352823/1‑100 (MQ=255)
                                                                                     aaaGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTa           >  1:1058211/1‑100 (MQ=255)
                                                                                      aaGAGTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTAt          <  1:124588/100‑1 (MQ=255)
                                                                                       agagTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATc         <  1:3656875/100‑1 (MQ=255)
                                                                                          gTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCttt      <  2:2621439/100‑1 (MQ=255)
                                                                                          gTGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCttt      <  2:4865152/100‑1 (MQ=255)
                                                                                           tGCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCcttt     <  2:3886436/100‑1 (MQ=255)
                                                                                            gCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTa    <  1:2810580/100‑1 (MQ=255)
                                                                                            gCACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTa    >  2:5189872/1‑100 (MQ=255)
                                                                                             cACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAt   >  1:1825337/1‑100 (MQ=255)
                                                                                             cACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAt   <  1:2461034/100‑1 (MQ=255)
                                                                                             cACTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAt   <  1:4035286/100‑1 (MQ=255)
                                                                                              aCTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTAtt  <  1:2233071/100‑1 (MQ=255)
                                                                                              aCTGCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTAGTGCATCAACTATCTTTTAtt  >  1:5220794/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ATTGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATT  >  NC_000913/313178‑313371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: