Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,635,996 C→T R454H (CGT→CAT)  der ← GTPase; multicopy suppressor of ftsJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,635,9960CT97.3% 753.9 / 2.8 222R454H (CGT→CAT) derGTPase; multicopy suppressor of ftsJ
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base T (111/105);  total (114/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

ATGTGCTTCATCAGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATACGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGA  >  NC_000913/2635900‑2636091
                                                                                                |                                                                                               
aTGTGCTTCATCAGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGaa                                                                                              <  1:1313753/100‑1 (MQ=255)
 tgtgCTTCATCAGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAAt                                                                                             <  1:628675/100‑1 (MQ=255)
    gCTTCATCAGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCgg                                                                                          >  1:3989610/1‑100 (MQ=255)
    gCTTCATCAGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCgg                                                                                          >  1:1504582/1‑100 (MQ=255)
     cTTCATCAGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGc                                                                                         >  2:4078915/1‑100 (MQ=255)
     cTTCATCAGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGc                                                                                         <  1:3292242/100‑1 (MQ=255)
     cTTCATCAGACGCTTACGTTTACGCATCTGGGTTGGCGCCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGc                                                                                         >  2:4890527/1‑100 (MQ=255)
      ttCATCAGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCg                                                                                        >  1:5268505/1‑100 (MQ=255)
        catcaGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGAt                                                                                      <  1:4714075/100‑1 (MQ=255)
        catcaGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGAt                                                                                      >  1:1872032/1‑100 (MQ=255)
         atcaGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATc                                                                                     <  1:4386982/100‑1 (MQ=255)
          tcaGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATcc                                                                                    >  2:4297581/1‑100 (MQ=255)
          tcaGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATcc                                                                                    <  1:253661/100‑1 (MQ=255)
           caGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATccc                                                                                   <  2:4610145/100‑1 (MQ=255)
             gACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCAt                                                                                 >  1:5482331/1‑100 (MQ=255)
              aCGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCAtt                                                                                <  1:1807021/100‑1 (MQ=255)
                 cTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACg                                                                             >  2:236669/1‑100 (MQ=255)
                   tACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTc                                                                           <  1:4144095/100‑1 (MQ=255)
                    aCGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTcc                                                                          <  1:364181/100‑1 (MQ=255)
                    aCGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTcc                                                                          <  2:2378687/100‑1 (MQ=255)
                    aCGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTcc                                                                          <  2:1756721/100‑1 (MQ=255)
                     cGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCa                                                                         >  2:3066899/1‑100 (MQ=255)
                     cGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCa                                                                         >  2:4046948/1‑100 (MQ=255)
                      gTTTACGCATCTGTGTTGGCGTCAGGGTGGTACGCTTATTCGCATACGGGTTTTCCCCCACTCTGAACTGAATATGAATCGCCGATCCCATTACGACCAg                                                                        >  2:3292215/1‑100 (MQ=255)
                       tttACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGc                                                                       <  2:1657139/100‑1 (MQ=255)
                        ttACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCg                                                                      >  1:708575/1‑100 (MQ=255)
                        ttACGCATCTGGGGTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCg                                                                      <  2:1130033/100‑1 (MQ=255)
                           cGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGAtt                                                                   <  1:2486446/100‑1 (MQ=255)
                            gCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGAttt                                                                  >  2:358379/1‑100 (MQ=255)
                             cATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTg                                                                 <  2:2278241/100‑1 (MQ=255)
                                cTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCgg                                                              >  1:770664/1‑100 (MQ=255)
                                 tGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGa                                                             >  2:3517742/1‑100 (MQ=255)
                                  gggTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGaa                                                            <  2:1291772/100‑1 (MQ=255)
                                   ggTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAag                                                           <  1:1076686/100‑1 (MQ=255)
                                   ggTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAag                                                           >  2:3709726/1‑100 (MQ=255)
                                   ggTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAag                                                           >  2:53931/1‑100 (MQ=255)
                                    gTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAagt                                                          >  1:4660403/1‑100 (MQ=255)
                                       ggCGTCAGGGTGTTAGGCTTATTCGCATACGGGTATTCACCTGCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGGTTTTCGGAagtagt                                                       >  2:2236573/1‑100 (MQ=255)
                                       ggCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAagtagt                                                       >  1:3006306/1‑100 (MQ=255)
                                        gCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGtt                                                      >  1:4625325/1‑100 (MQ=255)
                                         cGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTtc                                                     <  1:498451/100‑1 (MQ=255)
                                         cGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTtc                                                     <  1:3794998/100‑1 (MQ=255)
                                          gTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTtca                                                    >  2:2599062/1‑100 (MQ=255)
                                          gTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTtca                                                    <  2:1485492/100‑1 (MQ=255)
                                            cAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTtcatc                                                  >  1:4932603/1‑100 (MQ=255)
                                             aGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTtcatca                                                 <  2:4728540/100‑1 (MQ=255)
                                              gggTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCaa                                                <  2:3168390/100‑1 (MQ=255)
                                                gtgtTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGt                                              <  1:2736158/100‑1 (MQ=255)
                                                 tgtTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTa                                             <  2:4138825/100‑1 (MQ=255)
                                                  gtTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAg                                            >  1:957270/1‑100 (MQ=255)
                                                   ttACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAgc                                           >  2:4439886/1‑100 (MQ=255)
                                                    tACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAgcg                                          >  2:5137316/1‑100 (MQ=255)
                                                    tACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAgcg                                          >  2:1580797/1‑100 (MQ=255)
                                                    tACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAgcg                                          >  2:3570181/1‑100 (MQ=255)
                                                    tACGCTTATTCGCATACGGGTTTTCCCCTTCTTTAAACTGAATATGAATCGTCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAgcg                                          <  1:5006143/100‑1 (MQ=255)
                                                      cGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCt                                        <  2:3375433/100‑1 (MQ=255)
                                                      cGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCt                                        >  1:2983255/1‑100 (MQ=255)
                                                       gCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCtt                                       >  2:3788239/1‑100 (MQ=255)
                                                         ttATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGt                                     >  2:2428847/1‑100 (MQ=255)
                                                          tATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTCTa                                    >  2:1151125/1‑100 (MQ=255)
                                                          tATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATACGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTa                                    >  1:1523767/1‑100 (MQ=255)
                                                           aTTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAg                                   <  2:2827619/100‑1 (MQ=255)
                                                           aTTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAg                                   >  1:2413010/1‑100 (MQ=255)
                                                            ttCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAgg                                  >  1:5519397/1‑100 (MQ=255)
                                                            ctCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAgg                                  <  2:4218186/99‑1 (MQ=255)
                                                             tCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGa                                 >  1:1934920/1‑100 (MQ=255)
                                                             tCGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGa                                 <  1:4767847/100‑1 (MQ=255)
                                                              cGCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATTAAGTAGCGCTTGTAGGaa                                >  2:2898786/1‑100 (MQ=255)
                                                               gCATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAAt                               <  2:3910350/100‑1 (MQ=255)
                                                                cATACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATc                              <  1:2624659/100‑1 (MQ=255)
                                                                 aTACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATca                             >  1:452211/1‑100 (MQ=255)
                                                                 aTACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATca                             <  2:4626375/100‑1 (MQ=255)
                                                                 aTACGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATca                             >  2:2713478/1‑100 (MQ=255)
                                                                    cGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATcaggc                          <  2:2683355/100‑1 (MQ=255)
                                                                    cGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATcaggc                          <  2:1600506/100‑1 (MQ=255)
                                                                    cGGGTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATcaggc                          >  2:1027233/1‑100 (MQ=255)
                                                                     gggTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATcaggca                         <  1:2936249/100‑1 (MQ=255)
                                                                      ggTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATcaggcag                        >  1:1590866/1‑100 (MQ=255)
                                                                       gTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATcaggcagg                       <  2:3270193/100‑1 (MQ=255)
                                                                       gTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATcaggcagg                       <  2:2156154/100‑1 (MQ=255)
                                                                       gTTTTCCCCTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTCGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATcaggcagg                       >  2:3354533/1‑100 (MQ=255)
                                                                         tttCCCCTTCTTTGAACTGAATACGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTc                     <  1:3532539/100‑1 (MQ=255)
                                                                            ccccTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCttt                  >  2:3662632/1‑100 (MQ=255)
                                                                            ccccTTCTTTGAACTGAATATGAATCGGCGAACCCATTACGTCCAGCCATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCttt                  >  1:5467564/1‑100 (MQ=255)
                                                                             cccTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTc                 >  1:5035485/1‑100 (MQ=255)
                                                                              ccTTCTTTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCa                >  2:702183/1‑100 (MQ=255)
                                                                               cttcttTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCATGCAGGTCTTTCAc               <  2:589394/100‑1 (MQ=255)
                                                                               cttcttTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCAc               >  1:2069432/1‑100 (MQ=255)
                                                                                ttcttTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCAcc              <  1:4251408/100‑1 (MQ=255)
                                                                                ttcttTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCAcc              <  2:5192708/100‑1 (MQ=255)
                                                                                 tcttTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCt             <  1:3759193/100‑1 (MQ=255)
                                                                                  cttTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTg            <  2:3010679/100‑1 (MQ=255)
                                                                                  cttTGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTg            >  2:1260631/1‑100 (MQ=255)
                                                                                    ttGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGAt          <  2:3453963/100‑1 (MQ=255)
                                                                                     tGAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGAtt         <  1:3095670/100‑1 (MQ=255)
                                                                                      gAACTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTa        >  2:4964850/1‑100 (MQ=255)
                                                                                       aaCTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTAc       <  2:3220960/100‑1 (MQ=255)
                                                                                         cTGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCg     >  2:826932/1‑100 (MQ=255)
                                                                                          tGAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGt    <  1:736675/100‑1 (MQ=255)
                                                                                           gAATATGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTg   >  2:791497/1‑100 (MQ=255)
                                                                                                |                                                                                               
ATGTGCTTCATCAGACGCTTACGTTTACGCATCTGGGTTGGCGTCAGGGTGTTACGCTTATTCGCATACGGGTTTTCCCCTTCTTTGAACTGAATACGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGA  >  NC_000913/2635900‑2636091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑