Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,082,025 A→C Y38S (TAC→TCC)  loiP → Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,082,0250AC94.7% 688.0 / 14.9 209Y38S (TAC→TCC) loiPPhe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein
Reads supporting (aligned to +/- strand):  ref base A (4/7);  new base C (109/88);  total (114/95)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

TATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGC  >  NC_000913/3081929‑3082121
                                                                                                |                                                                                                
tATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAg                                                                                               >  1:2062101/1‑100 (MQ=255)
  ttGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGtt                                                                                             <  2:3618110/100‑1 (MQ=255)
    ggTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTg                                                                                           <  2:3891609/100‑1 (MQ=255)
       aGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGt                                                                                        >  1:1789146/1‑100 (MQ=255)
        gCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTg                                                                                       >  2:1270629/1‑100 (MQ=255)
         cAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCAACAGCAGCGCAAGCTTTTCAGGCTTCCAGTTTAAGtta                                                                                      >  2:4409629/1‑98 (MQ=255)
           aTGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATg                                                                                    >  1:4266498/1‑100 (MQ=255)
            tGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATgc                                                                                   >  2:2222607/1‑100 (MQ=255)
            tGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATgc                                                                                   <  2:2689930/100‑1 (MQ=255)
                cGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAg                                                                               >  1:3910980/1‑100 (MQ=255)
                cGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAg                                                                               >  2:4836151/1‑100 (MQ=255)
                 gTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAgg                                                                              <  1:5291539/100‑1 (MQ=255)
                 gTGGAAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCCGGCTTCCAGTTTGAGTCCTGCGCAgg                                                                              >  2:4668380/1‑100 (MQ=255)
                  tGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGt                                                                             <  1:4784019/100‑1 (MQ=255)
                  tGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGt                                                                             >  1:4868954/1‑100 (MQ=255)
                    gCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGa                                                                           >  2:4965087/1‑100 (MQ=255)
                     cAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGaa                                                                          >  2:3747809/1‑100 (MQ=255)
                          gTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAAccc                                                                     >  2:3321698/1‑100 (MQ=255)
                          gTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAAccc                                                                     <  1:1687510/100‑1 (MQ=255)
                          gTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAAccc                                                                     <  2:3289241/100‑1 (MQ=255)
                           tACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGCAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCt                                                                    <  2:5112375/100‑1 (MQ=255)
                            actgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTg                                                                   <  1:3001795/100‑1 (MQ=255)
                             ctgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGa                                                                  >  1:555653/1‑100 (MQ=255)
                             ctgactgGTTGCCAGAACATGGACTCCAACGGACTGCTCCCCTCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGa                                                                  >  2:2207418/1‑100 (MQ=255)
                              tgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAg                                                                 >  1:2766270/1‑100 (MQ=255)
                              tgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAg                                                                 >  2:3574713/1‑100 (MQ=255)
                              tgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAg                                                                 <  1:3164538/100‑1 (MQ=255)
                              tgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAg                                                                 <  1:1242142/100‑1 (MQ=255)
                              tgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAg                                                                 <  2:5380175/100‑1 (MQ=255)
                               gactgGTTGCCAGAATATGGACTCCAACGTACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGc                                                                <  1:5092121/100‑1 (MQ=255)
                               gactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGc                                                                <  1:5505722/100‑1 (MQ=255)
                               gactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGc                                                                <  1:2450839/100‑1 (MQ=255)
                                taccgTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCg                                                               <  2:4315474/96‑1 (MQ=255)
                                  tgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGAt                                                             >  2:289880/1‑100 (MQ=255)
                                   ggTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATc                                                            <  1:2296181/100‑1 (MQ=255)
                                    gTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCa                                                           <  1:3700043/100‑1 (MQ=255)
                                    gTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCa                                                           <  2:1168836/100‑1 (MQ=255)
                                      tGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAgg                                                         <  1:5541457/100‑1 (MQ=255)
                                           gAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGt                                                    >  1:1609860/1‑100 (MQ=255)
                                            aaTATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTc                                                   <  2:1768761/100‑1 (MQ=255)
                                             atatGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCa                                                  <  1:4220173/100‑1 (MQ=255)
                                              tatGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCATGCATGTCAg                                                 <  1:5150930/100‑1 (MQ=255)
                                               atGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAgg                                                >  1:4703686/1‑100 (MQ=255)
                                               atGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAgg                                                <  2:4246781/100‑1 (MQ=255)
                                                tGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGga                                               <  1:999809/100‑1 (MQ=255)
                                                tGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGga                                               <  2:2823851/100‑1 (MQ=255)
                                                 ggACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGgag                                              >  2:3353424/1‑100 (MQ=255)
                                                 ggACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGgag                                              <  2:2106675/100‑1 (MQ=255)
                                                    cTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATg                                           >  1:3583751/1‑100 (MQ=255)
                                                    cTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATg                                           <  1:460640/100‑1 (MQ=255)
                                                    cTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTATTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATg                                           >  1:3954761/1‑100 (MQ=255)
                                                     tCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGCGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATgg                                          >  2:756588/1‑100 (MQ=255)
                                                     tCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATgg                                          >  2:3538075/1‑100 (MQ=255)
                                                        aaCGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACa                                       <  1:633674/100‑1 (MQ=255)
                                                         aCGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAg                                      >  1:2455989/1‑100 (MQ=255)
                                                          cGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGc                                     <  1:5437795/100‑1 (MQ=255)
                                                           ggACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCa                                    >  1:212209/1‑100 (MQ=255)
                                                            gACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCaa                                   >  1:857030/1‑100 (MQ=255)
                                                            gACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCaa                                   >  1:3541316/1‑100 (MQ=255)
                                                              cTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAgg                                 >  1:922108/1‑100 (MQ=255)
                                                              cTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAgg                                 >  2:3428226/1‑100 (MQ=255)
                                                              cTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAgg                                 >  1:4553489/1‑100 (MQ=255)
                                                               tGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGc                                >  1:1946171/1‑100 (MQ=255)
                                                               tGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGc                                <  1:4362016/100‑1 (MQ=255)
                                                                nctctCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcg                               >  1:4455872/2‑100 (MQ=255)
                                                                gCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcg                               >  1:3436146/1‑100 (MQ=255)
                                                                gCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcg                               >  1:199168/1‑100 (MQ=255)
                                                                gCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcg                               >  2:4351394/1‑100 (MQ=255)
                                                                 ctctCATCAGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcga                              >  1:4939335/1‑100 (MQ=255)
                                                                     catcaGGAGCGGAAGCTTTTCAGGCTTTCAGTTTGAGTGATGCGCAGGAGAAAAACCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGcgacgct                          >  2:5476623/1‑98 (MQ=255)
                                                                     catcaGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGAt                          >  2:710205/1‑100 (MQ=255)
                                                                     catcaGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGAt                          >  1:5177198/1‑100 (MQ=255)
                                                                        caGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgc                       >  2:130886/1‑100 (MQ=255)
                                                                        caGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgc                       >  1:2546239/1‑100 (MQ=255)
                                                                        caGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgc                       >  1:2846758/1‑100 (MQ=255)
                                                                         aGGAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgcg                      <  1:2074862/100‑1 (MQ=255)
                                                                          ggAGCGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTgcgc                     >  2:4132800/1‑100 (MQ=255)
                                                                             gtggAAGCTTATCACGCTCACAGTTTGAGAGATGTGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATCGAAAGCAAGGCAACGCCTGCgccag                  <  2:1837124/98‑1 (MQ=255)
                                                                              cGGAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagc                 <  1:1602144/100‑1 (MQ=255)
                                                                               ggAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagcc                >  1:728901/1‑100 (MQ=255)
                                                                               ggAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagcc                <  1:1067923/100‑1 (MQ=255)
                                                                               ggAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCcccagcc                >  2:230799/1‑100 (MQ=255)
                                                                                gAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagcca               >  1:5569172/1‑100 (MQ=255)
                                                                                gAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagcca               >  2:2882836/1‑100 (MQ=255)
                                                                                gAAGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCgccagcca               >  1:2169404/1‑100 (MQ=255)
                                                                                  aGCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAAt             <  2:2431564/100‑1 (MQ=255)
                                                                                   gCTTTTCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATa            >  1:4048761/1‑100 (MQ=255)
                                                                                     ttttCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGc          <  1:269148/100‑1 (MQ=255)
                                                                                      tttCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCg         <  2:4081297/100‑1 (MQ=255)
                                                                                      tttCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCg         <  1:2572614/100‑1 (MQ=255)
                                                                                      tttCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCg         <  2:4644043/100‑1 (MQ=255)
                                                                                      tttCAAGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCg         <  2:3389262/100‑1 (MQ=255)
                                                                                       ttCAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGa        <  1:3384859/100‑1 (MQ=255)
                                                                                         cAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAAt      <  2:3774015/100‑1 (MQ=255)
                                                                                         cAGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAAt      >  1:2859467/1‑100 (MQ=255)
                                                                                          aGGCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATa     <  2:846069/100‑1 (MQ=255)
                                                                                           ggCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATAc    >  2:4510386/1‑100 (MQ=255)
                                                                                            gCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACg   >  2:1583209/1‑100 (MQ=255)
                                                                                            gCTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACg   <  2:3184704/100‑1 (MQ=255)
                                                                                             cTTCCAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGc  >  2:3011672/1‑100 (MQ=255)
                                                                                                |                                                                                                
TATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGC  >  NC_000913/3081929‑3082121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: