Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,224,462 T→G T610T (ACT→ACG)  ebgA → evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,224,4620TG99.1% 785.5 / ‑4.5 219T610T (ACT→ACG) ebgAevolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (106/111);  total (107/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

TTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGC  >  NC_000913/3224365‑3224557
                                                                                                 |                                                                                               
ntGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGAcgcg                                                                                               >  2:1425545/2‑100 (MQ=255)
 tGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACgcgc                                                                                              >  1:2290658/1‑100 (MQ=255)
 ngATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACgcgc                                                                                              >  1:4421617/2‑100 (MQ=255)
  gATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGAcgcgcg                                                                                             <  2:5473410/100‑1 (MQ=255)
   aTGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCgcgg                                                                                            >  2:2210525/1‑100 (MQ=255)
        ttGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGt                                                                                       <  1:183178/100‑1 (MQ=255)
              tATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAg                                                                                 <  1:2006790/100‑1 (MQ=255)
                ttCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGGTGAAAGTc                                                                               >  2:5302786/1‑100 (MQ=255)
                 tCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCg                                                                              <  2:2819634/100‑1 (MQ=255)
                  ccGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGa                                                                             >  2:4111503/1‑100 (MQ=255)
                   cGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGaa                                                                            >  2:4406739/1‑100 (MQ=255)
                   cGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGaa                                                                            >  2:740040/1‑100 (MQ=255)
                   cGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGGCGaa                                                                            >  1:1642779/1‑100 (MQ=255)
                     aTCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGaaaa                                                                          <  1:1066027/100‑1 (MQ=255)
                     aTCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGaaaa                                                                          <  2:522854/100‑1 (MQ=255)
                     aTCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGaaaa                                                                          >  1:782877/1‑100 (MQ=255)
                       cAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATa                                                                        <  1:3294853/100‑1 (MQ=255)
                          aCGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAAc                                                                     <  2:1178776/100‑1 (MQ=255)
                          aCGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAAc                                                                     <  2:5101794/100‑1 (MQ=255)
                           cGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACt                                                                    >  2:1522461/1‑100 (MQ=255)
                           cGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACt                                                                    >  2:1218606/1‑100 (MQ=255)
                           cGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACt                                                                    >  1:1132653/1‑100 (MQ=255)
                           cGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACt                                                                    <  1:3860279/100‑1 (MQ=255)
                             ccGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACtgt                                                                  >  2:1307924/1‑100 (MQ=255)
                             ccGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACtgt                                                                  >  2:1131556/1‑100 (MQ=255)
                               gggACCGGGCCTGCAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTgg                                                                >  1:2084532/1‑100 (MQ=255)
                               gggACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTgg                                                                <  1:3068381/100‑1 (MQ=255)
                               gggACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTgg                                                                <  1:5013949/100‑1 (MQ=255)
                               gggACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTgg                                                                >  2:2013155/1‑100 (MQ=255)
                               gggACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGATAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTgg                                                                <  2:4684630/100‑1 (MQ=255)
                                ggACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGt                                                               <  1:2484843/100‑1 (MQ=255)
                                 gACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGtt                                                              <  2:3714998/100‑1 (MQ=255)
                                  aCCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGttt                                                             >  1:2941543/1‑100 (MQ=255)
                                   ccGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTa                                                            >  2:1636815/1‑100 (MQ=255)
                                   ccGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTa                                                            >  2:4231173/1‑100 (MQ=255)
                                    cGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTAc                                                           <  2:13201/100‑1 (MQ=255)
                                      tgCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCa                                                         <  1:1173098/99‑1 (MQ=255)
                                      ggCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCa                                                         <  1:2620206/100‑1 (MQ=255)
                                        ccTGAAAGCGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACg                                                       >  1:1073098/1‑100 (MQ=255)
                                         cTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGACCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGc                                                      >  1:3129916/1‑100 (MQ=255)
                                          tGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCt                                                     >  1:61990/1‑100 (MQ=255)
                                           gAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCtt                                                    >  1:3403239/1‑100 (MQ=255)
                                            aaaGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtg                                                   <  1:1540340/100‑1 (MQ=255)
                                            aaaGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtg                                                   >  2:3838102/1‑100 (MQ=255)
                                            aaaGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTAAACCAcagttt                                                   <  1:2575823/100‑6 (MQ=255)
                                            aaaGAGTACAAACAGGTAATCGCGCCGGTAAAAATCCACGCGTGGGATCTGACGCGTGGCGAGTTGAAAGTCAAAAATAAACTGTGGTTTACCACGCTtg                                                   <  2:1893283/100‑1 (MQ=255)
                                              agagTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtgat                                                 >  1:459723/1‑100 (MQ=255)
                                              agagTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtgat                                                 <  2:2913865/100‑1 (MQ=255)
                                              agagTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtgat                                                 <  1:3668363/100‑1 (MQ=255)
                                              agagTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtgat                                                 >  2:4863391/1‑100 (MQ=255)
                                               gagTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtgatg                                                >  1:1712904/1‑100 (MQ=255)
                                               gagTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtgatg                                                <  1:3533336/100‑1 (MQ=255)
                                               gagTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtgatg                                                <  1:3161711/100‑1 (MQ=255)
                                                 gTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGAc                                              >  2:3787710/1‑100 (MQ=255)
                                                  tACAAACAGGTGATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAGCTGTGGTTTACCACGCTTGATGACt                                             <  2:4387526/100‑1 (MQ=255)
                                                   aCAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTa                                            >  2:178763/1‑100 (MQ=255)
                                                   aCAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGAGCGGACGCGCGGCGCGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtgatggcca                                            >  1:4734655/1‑96 (MQ=255)
                                                      aaCAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTacac                                         >  1:1109341/1‑100 (MQ=255)
                                                        cgatctATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACAccc                                       <  2:2516547/95‑1 (MQ=255)
                                                        cAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACAccc                                       >  1:1476037/1‑100 (MQ=255)
                                                          ggTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTg                                     >  1:837390/1‑100 (MQ=255)
                                                          ggTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTg                                     >  1:5517234/1‑100 (MQ=255)
                                                          ggTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTg                                     >  1:4092004/1‑100 (MQ=255)
                                                          ggTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTg                                     <  1:3804522/100‑1 (MQ=255)
                                                          ggTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTg                                     <  1:1516695/100‑1 (MQ=255)
                                                          ggTTATCGCGCCGGTAAAAATCCACGCGCGGAATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTg                                     >  1:4134458/1‑100 (MQ=255)
                                                          ggTTATCGCGCCAGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTg                                     >  2:3266367/1‑100 (MQ=255)
                                                           gTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGc                                    <  2:3411671/100‑1 (MQ=255)
                                                           gTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGc                                    >  2:2545724/1‑100 (MQ=255)
                                                              aTCGCGCTTGTAAAAATCCACCCCCGGGATCGGAAGCTCCGCGAGTTGAAATTCTAAAATAATCTGTGCTTTACCACTCGTGATGACTACACCCTGCACg                                 <  2:3890971/100‑1 (MQ=255)
                                                              aTCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACg                                 >  2:956892/1‑100 (MQ=255)
                                                              aTCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACg                                 <  1:932982/100‑1 (MQ=255)
                                                               tCGCGCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACgn                                <  1:4449024/100‑2 (MQ=255)
                                                                cgcgCCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCa                               >  1:3442068/1‑100 (MQ=255)
                                                                  cgcCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCaga                             <  2:3410056/100‑1 (MQ=255)
                                                                   gcCGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCagag                            <  1:5529799/100‑1 (MQ=255)
                                                                    ccGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAgg                           <  2:1694356/100‑1 (MQ=255)
                                                                    ccGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAgg                           >  1:271908/1‑100 (MQ=255)
                                                                    ccGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAgg                           <  1:4050898/100‑1 (MQ=255)
                                                                     cGGTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGt                          >  2:3053885/1‑100 (MQ=255)
                                                                      ggTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTg                         <  1:5366976/100‑1 (MQ=255)
                                                                       gTAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgc                        <  1:1332135/100‑1 (MQ=255)
                                                                        tAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgcg                       <  2:1739513/100‑1 (MQ=255)
                                                                        tAAAAATCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgcg                       <  2:843988/100‑1 (MQ=255)
                                                                          aaaaTCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgcgcg                     >  1:2864082/1‑100 (MQ=255)
                                                                          aaaaTCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgcgcg                     >  1:2152188/1‑100 (MQ=255)
                                                                           aaaTCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGCTGCATCCACCCTGCAAGCAGAGGTgcgaga                    >  1:5564638/1‑97 (MQ=255)
                                                                            aaTCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGcc                   <  1:4797259/100‑1 (MQ=255)
                                                                              tCCACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGa                 <  1:4730355/100‑1 (MQ=255)
                                                                                cACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg               <  2:4065259/100‑1 (MQ=255)
                                                                                cACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg               >  2:458474/1‑100 (MQ=255)
                                                                                cACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg               >  2:5326249/1‑100 (MQ=255)
                                                                                cACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg               <  1:2442421/100‑1 (MQ=255)
                                                                                cACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg               >  2:2401864/1‑100 (MQ=255)
                                                                                cACGCGCGGGATCTGACGCGCGGCGAGTTGAAAGGCGAAAATAAACTGTCGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg               <  2:5406462/100‑1 (MQ=255)
                                                                                 aCGCGCGGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAgg              <  1:5095086/100‑1 (MQ=255)
                                                                                    cgcgGGATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGa           >  1:2913819/1‑100 (MQ=255)
                                                                                       gggATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAAc        <  1:4932572/100‑1 (MQ=255)
                                                                                       gggATCTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAAc        <  2:2893359/100‑1 (MQ=255)
                                                                                            cTGACGCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTcg   >  1:498852/1‑100 (MQ=255)
                                                                                                 |                                                                                               
TTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGC  >  NC_000913/3224365‑3224557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑