Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,468,138 T→G G572G (GGA→GGC chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,468,1380TG98.4% 670.5 / ‑5.0 190G572G (GGA→GGCchiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (81/106);  total (82/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

CAGCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTTCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGCGA  >  NC_000913/3468041‑3468234
                                                                                                 |                                                                                                
cAGCCAGATCCGGGATCGTATTCAGCGTAAAGTCAACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGcc                                                                                                <  1:2490878/100‑1 (MQ=255)
 aGCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCg                                                                                               <  1:4844397/100‑1 (MQ=255)
  gCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGTGTACCTACGCGCTCTTTGGTGCCgg                                                                                              <  2:689761/100‑1 (MQ=255)
  gCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCgg                                                                                              <  2:340574/100‑1 (MQ=255)
  gCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCgg                                                                                              >  2:5493634/1‑100 (MQ=255)
  gCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCgg                                                                                              <  2:3019260/100‑1 (MQ=255)
  gCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCgg                                                                                              <  1:463141/100‑1 (MQ=255)
    cAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTc                                                                                            >  2:2763253/1‑100 (MQ=255)
      gATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCa                                                                                          >  2:2048562/1‑100 (MQ=255)
       aTCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAg                                                                                         <  2:2088967/100‑1 (MQ=255)
        tCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGt                                                                                        <  2:1592572/100‑1 (MQ=255)
          cGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTca                                                                                      <  1:4932307/100‑1 (MQ=255)
           gggATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTcaa                                                                                     >  2:2792139/1‑100 (MQ=255)
            ggATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTcaac                                                                                    <  2:397413/100‑1 (MQ=255)
            ggATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTcaac                                                                                    <  2:3344039/100‑1 (MQ=255)
             gATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTcaaca                                                                                   <  2:3302930/100‑1 (MQ=255)
             gATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTcaaca                                                                                   >  2:299148/1‑100 (MQ=255)
                cGTATTCAGCGTAGAGTCGACATACGGTGCATAAACGTGTGTCGGCCACGCTGTCGTCGGCGTACCGACGCGCTCTTTGGTGCCGGTGCAGTCAACaaaa                                                                                <  2:4105150/100‑1 (MQ=255)
                   aTTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAaca                                                                             <  1:751726/100‑1 (MQ=255)
                    ttCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAacan                                                                            <  1:4426498/100‑2 (MQ=255)
                     tCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACg                                                                           <  2:5016609/100‑1 (MQ=255)
                      cAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGc                                                                          >  1:1396426/1‑100 (MQ=255)
                         cGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCAc                                                                       >  1:3719016/1‑100 (MQ=255)
                              aGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCCCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGtt                                                                  >  1:1935427/1‑100 (MQ=255)
                               gTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTg                                                                 >  2:2405262/1‑100 (MQ=255)
                               gTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTg                                                                 <  1:3957901/100‑1 (MQ=255)
                               gTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTg                                                                 >  1:3706959/1‑100 (MQ=255)
                               gTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTg                                                                 >  2:158638/1‑100 (MQ=255)
                                  gACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTg                                                              <  1:91414/100‑1 (MQ=255)
                                  gACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTg                                                              <  2:454732/100‑1 (MQ=255)
                                  gACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTg                                                              <  1:5203893/100‑1 (MQ=255)
                                    cATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTc                                                            <  2:3643579/100‑1 (MQ=255)
                                    cATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTc                                                            <  1:1499504/100‑1 (MQ=255)
                                     aTACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCa                                                           <  1:2730571/100‑1 (MQ=255)
                                     aTACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCa                                                           <  1:2707099/100‑1 (MQ=255)
                                      tACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAg                                                          >  2:3521719/1‑100 (MQ=255)
                                      tACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAg                                                          >  1:3013645/1‑100 (MQ=255)
                                      tACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAg                                                          >  1:1732643/1‑100 (MQ=255)
                                       aCGGTGCATAAACGTGTTTCGGCCAAGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGa                                                         >  1:4986378/1‑100 (MQ=255)
                                        cGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGaa                                                        >  1:2506516/1‑100 (MQ=255)
                                         ggTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAg                                                       >  2:1157216/1‑100 (MQ=255)
                                         ggTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAg                                                       <  2:261840/100‑1 (MQ=255)
                                         ggTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAg                                                       >  1:101743/1‑100 (MQ=255)
                                          gTGCATAACCGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCGTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAgg                                                      <  1:1733419/100‑1 (MQ=255)
                                          gTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAGCAAAAACACGCCACGGGTTGCTGTCAGAAgg                                                      <  1:4468716/100‑1 (MQ=255)
                                          gTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAgg                                                      <  1:2031024/100‑1 (MQ=255)
                                          gTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAgg                                                      <  2:2670787/100‑1 (MQ=255)
                                          gTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAgg                                                      <  2:5289561/100‑1 (MQ=255)
                                           tGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGga                                                     <  2:883962/100‑1 (MQ=255)
                                           tGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGga                                                     >  1:1610471/1‑100 (MQ=255)
                                            gCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGgag                                                    <  1:3674581/100‑1 (MQ=255)
                                               tAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGaaa                                                 >  1:5109924/1‑100 (MQ=255)
                                               tAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGaaa                                                 >  2:586273/1‑100 (MQ=255)
                                                aaaCGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGaacc                                                >  2:4473483/1‑98 (MQ=255)
                                                  aCGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACtt                                              >  2:1752970/1‑100 (MQ=255)
                                                   cGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACttt                                             >  1:1833839/1‑100 (MQ=255)
                                                    gtgtTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTc                                            <  1:5521147/100‑1 (MQ=255)
                                                      gtTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTg                                          >  2:1235842/1‑100 (MQ=255)
                                                      gtTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTg                                          <  2:4912461/100‑1 (MQ=255)
                                                      gtTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTg                                          <  1:1877732/100‑1 (MQ=255)
                                                        ttCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCa                                        >  1:3432127/1‑100 (MQ=255)
                                                          cGGCCACGCCTTCTTCGGCGTACATACGCGCTCTTTGGTGCCTGTCCAGTCAACAAAAACGCGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACt                                      <  1:4856417/100‑1 (MQ=255)
                                                           ggCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACtt                                     >  1:3601817/1‑100 (MQ=255)
                                                            gCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACttt                                    >  2:5093762/1‑100 (MQ=255)
                                                            gCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACttt                                    >  1:1583045/1‑100 (MQ=255)
                                                            gCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACttt                                    <  1:1530102/100‑1 (MQ=255)
                                                             ccACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTa                                   >  1:2374463/1‑100 (MQ=255)
                                                             ccACGCTTTCTTCGGCGTACCTACGCGCGCTTTGGGGCCGGTCCAGTCAACAAAAACACGCAACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTa                                   <  1:1435992/100‑1 (MQ=255)
                                                              cACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTAc                                  <  2:2316121/100‑1 (MQ=255)
                                                                cGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTActc                                >  2:954897/1‑100 (MQ=255)
                                                                 gCTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTActct                               <  2:3231185/100‑1 (MQ=255)
                                                                  cTTTCTTCGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTg                              <  1:1088716/100‑1 (MQ=255)
                                                                    ttcttcGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTgag                            <  2:90226/100‑1 (MQ=255)
                                                                      cttcGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGaa                          >  2:4334579/1‑100 (MQ=255)
                                                                      cttcGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGaa                          >  1:1008322/1‑100 (MQ=255)
                                                                        tcGGCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAAt                        <  1:2459682/100‑1 (MQ=255)
                                                                           gCGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTa                     <  2:3568561/100‑1 (MQ=255)
                                                                            cGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAg                    >  1:4171607/1‑100 (MQ=255)
                                                                            cGTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGACAAACTTTCTGCACTTTACTCTGAGAAATGTAg                    >  1:2180544/1‑100 (MQ=255)
                                                                             gTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGt                   <  2:2628818/100‑1 (MQ=255)
                                                                             gTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGt                   <  2:1870140/100‑1 (MQ=255)
                                                                             gTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGt                   >  1:1196238/1‑100 (MQ=255)
                                                                             gTACCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGt                   <  2:5127845/100‑1 (MQ=255)
                                                                               aCCTACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTc                 <  2:4354893/100‑1 (MQ=255)
                                                                                  tACGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTcaca              >  1:2243832/1‑100 (MQ=255)
                                                                                   aCGCGCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCacac             <  1:3610426/100‑1 (MQ=255)
                                                                                    cgcgCTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACAcc            >  2:770691/1‑100 (MQ=255)
                                                                                    cgcgCTCTTTGGTGCCGGTCCAATCAACAAAAACACGCCACGGGTTGCTGCGAGAAGGAGAAACTTTCTAAACTTTACTCTGAGAAATGTCGCTCACAcc            >  2:177606/1‑100 (MQ=255)
                                                                                      cgcTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGt          >  1:3659794/1‑100 (MQ=255)
                                                                                       gcTCTTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGtt         <  1:5438747/100‑1 (MQ=255)
                                                                                        ttctTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTa        <  2:3620335/99‑1 (MQ=255)
                                                                                        ctctTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTa        <  2:479151/100‑1 (MQ=255)
                                                                                        ctctTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTa        >  2:2013774/1‑100 (MQ=255)
                                                                                         tctTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTaa       >  1:3653995/1‑100 (MQ=255)
                                                                                          ctTTGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTaaa      >  1:2843621/1‑100 (MQ=255)
                                                                                           tttGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAg     <  2:1346237/100‑1 (MQ=255)
                                                                                            ttGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGGAACTTTCTGCACTTTTCTCTGAGAAATGTAGTTCACACCGTTAAAGc    <  2:221759/100‑1 (MQ=255)
                                                                                             tGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGCg   <  2:1563620/100‑1 (MQ=255)
                                                                                             tGGTGCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGCg   <  1:2070608/100‑1 (MQ=255)
                                                                                              ggTGCCGGTCCAGTCAACTAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCCGCGAAATGTAGTTCACACCGTTAAAGCGa  >  2:5453364/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CAGCCAGATCCGGGATCGTATTCAGCGTAAAGTCGACATACGGTGCATAAACGTGTTTCGGCCACGCTTTCTTCGGCGTACCTACGCGCTCTTTGGTTCCGGTCCAGTCAACAAAAACACGCCACGGGTTGCTGTCAGAAGGAGAAACTTTCTGCACTTTACTCTGAGAAATGTAGTTCACACCGTTAAAGCGA  >  NC_000913/3468041‑3468234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: