Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,716,242 A→C V51V (GTT→GTG bisC ← biotin sulfoxide reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,716,2420AC99.0% 728.1 / ‑4.3 208V51V (GTT→GTGbisCbiotin sulfoxide reductase
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base C (105/100);  total (106/102)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 208 total aligned reads displayed.

GAACAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGAACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCAGC  >  NC_000913/3716145‑3716338
                                                                                                 |                                                                                                
 aaCAAATTGATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCAcc                                                                                               <  1:5312789/100‑1 (MQ=255)
 aaCAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCAcc                                                                                               >  2:905938/1‑100 (MQ=255)
 aaCAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCAcc                                                                                               <  2:70482/100‑1 (MQ=255)
   cAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTg                                                                                             >  2:309043/1‑100 (MQ=255)
   cAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTg                                                                                             >  1:3297997/1‑100 (MQ=255)
   cAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTg                                                                                             >  1:1923800/1‑100 (MQ=255)
   cAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTg                                                                                             <  2:678541/100‑1 (MQ=255)
     aaTTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGt                                                                                           >  1:4553014/1‑100 (MQ=255)
     aaTTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGt                                                                                           >  1:2423294/1‑100 (MQ=255)
     aaTTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGt                                                                                           >  2:1653706/1‑100 (MQ=255)
       ttCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTcg                                                                                         <  1:5019099/100‑1 (MQ=255)
         cATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTcgcg                                                                                       >  1:804312/1‑100 (MQ=255)
         cATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTcgcg                                                                                       <  2:1303316/100‑1 (MQ=255)
         cATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTcgcg                                                                                       <  2:1586152/100‑1 (MQ=255)
         cATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTcgcg                                                                                       <  1:3317896/100‑1 (MQ=255)
             cTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAAcc                                                                                   <  2:3799101/100‑1 (MQ=255)
                cccACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACcgcg                                                                                >  1:1464014/1‑100 (MQ=255)
                   aCGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGctc                                                                             <  1:2886175/100‑1 (MQ=255)
                     gAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTATCCGCGTATTGCTTTGCACCTGGTCGCGAACCGCGCTCTg                                                                           <  2:455581/100‑1 (MQ=255)
                     gAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTg                                                                           <  1:194966/100‑1 (MQ=255)
                        tGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCaa                                                                        <  2:829235/100‑1 (MQ=255)
                         gCCTTGCGTGTTTTCCGGTGATGCAAGATAGCCTTTTCGCTCTTTTGGGAATCGTGCCCGCGCATTGCTGGGCACCTGGTCGCGAACCGCGCTCTGCAAg                                                                       <  1:2030056/100‑1 (MQ=255)
                         gCCTTGCGGGTTTTGCGGTGACGCAAGAAAGCCTTTTCGCGCCATTGGAAATCGTACCCGCGTATTACTGTGCACCTGGTCGCGAACCGCGCTCTGCAAg                                                                       <  2:299247/100‑1 (MQ=255)
                         gCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAg                                                                       <  1:5425321/100‑1 (MQ=255)
                          ccTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCACgg                                                                      >  1:4187078/1‑100 (MQ=255)
                          ccTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAgg                                                                      >  2:2866129/1‑100 (MQ=255)
                          ccTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAgg                                                                      >  2:3188782/1‑100 (MQ=255)
                          ccTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAgg                                                                      >  2:4946444/1‑100 (MQ=255)
                          ccTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAgg                                                                      >  1:4343323/1‑100 (MQ=255)
                            ttGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAg                                                                    >  1:953536/1‑100 (MQ=255)
                            ttGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAg                                                                    <  1:5533992/100‑1 (MQ=255)
                            ttGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAg                                                                    <  2:583222/100‑1 (MQ=255)
                             tGCGGGTTTGCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGt                                                                   <  2:3668854/100‑1 (MQ=255)
                               cGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGttt                                                                 <  2:1162926/100‑1 (MQ=255)
                               cGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGttt                                                                 <  1:1659200/100‑1 (MQ=255)
                                 ggTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTc                                                               <  2:1934241/100‑1 (MQ=255)
                                   ttttCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCa                                                             >  2:4782215/1‑100 (MQ=255)
                                    tttCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCAt                                                            <  2:2354624/100‑1 (MQ=255)
                                      tCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAGATCGTACCCGCGTATTGCTGTGCACCTGGTCCCGAACCGCGCTCTGCAAGGAGTTTTCCATTc                                                          <  1:4461507/100‑1 (MQ=255)
                                      tCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTc                                                          >  1:4250440/1‑100 (MQ=255)
                                       ccGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTcc                                                         <  1:4906456/100‑1 (MQ=255)
                                       ccGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTcc                                                         >  1:3455953/1‑100 (MQ=255)
                                        cGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGTTCTGCAAGGAGTTTTCCATTCCt                                                        >  2:5016250/1‑100 (MQ=255)
                                        cGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGGTTTCCATTCCt                                                        >  2:3896039/1‑100 (MQ=255)
                                         ggTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCtg                                                       <  2:4211671/100‑1 (MQ=255)
                                         ggTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCtg                                                       <  2:3123232/100‑1 (MQ=255)
                                         ggTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCtg                                                       <  2:1237222/100‑1 (MQ=255)
                                         ggTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCtg                                                       <  2:4043262/100‑1 (MQ=255)
                                          gTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCtgt                                                      <  1:3197989/100‑1 (MQ=255)
                                            gACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTgg                                                    <  2:5397094/100‑1 (MQ=255)
                                            gACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTgg                                                    <  1:3254632/100‑1 (MQ=255)
                                            gACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTgg                                                    <  2:1902680/100‑1 (MQ=255)
                                            gACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGAACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTgg                                                    <  2:4468237/100‑1 (MQ=255)
                                             aCGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGc                                                   <  1:2200806/100‑1 (MQ=255)
                                              cGCAAGAAAGCCTTTTCGCACCATTTGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCt                                                  >  2:278113/1‑100 (MQ=255)
                                              cGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCt                                                  >  1:3538423/1‑100 (MQ=255)
                                              cGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCt                                                  >  1:3070183/1‑100 (MQ=255)
                                               gCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTa                                                 <  2:2609478/100‑1 (MQ=255)
                                               gCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTa                                                 >  2:127167/1‑100 (MQ=255)
                                               gCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTa                                                 >  1:2054732/1‑100 (MQ=255)
                                               gCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTa                                                 >  1:2524789/1‑100 (MQ=255)
                                                cAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTaa                                                >  1:3985095/1‑100 (MQ=255)
                                                    aaaGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAAcgcg                                            <  2:4172184/100‑1 (MQ=255)
                                                      aGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGcc                                          <  1:4508880/100‑1 (MQ=255)
                                                      aGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGcc                                          >  1:5521492/1‑100 (MQ=255)
                                                      aGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGcc                                          <  1:1485252/100‑1 (MQ=255)
                                                        ccTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCAc                                        <  2:3230886/100‑1 (MQ=255)
                                                          ttttCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCAcgc                                      >  1:79556/1‑100 (MQ=255)
                                                           tttCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCAcgcg                                     <  2:5483056/100‑1 (MQ=255)
                                                              cGCACCATTGGAAATCGTACCCGCGTATTGCTGTGATCCTGGTCCCGAACCGCGCGCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGc                                  <  2:1079266/100‑1 (MQ=255)
                                                               gCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCt                                 >  2:1681156/1‑100 (MQ=255)
                                                               gCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCt                                 >  2:2656671/1‑100 (MQ=255)
                                                               gCACCATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCt                                 >  1:755101/1‑100 (MQ=255)
                                                                 aCCATTGGAAATCTTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTaa                               >  2:903935/1‑100 (MQ=255)
                                                                  ccATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTaaa                              <  1:3650103/100‑1 (MQ=255)
                                                                  ccATTGGAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTaaa                              <  1:2219797/100‑1 (MQ=255)
                                                                       ggAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACgg                         >  1:1376625/1‑100 (MQ=255)
                                                                       ggAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACgg                         <  2:72196/100‑1 (MQ=255)
                                                                       ggAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACgg                         <  1:4896332/100‑1 (MQ=255)
                                                                        gAAATCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGGGCTAAACACGGt                        <  2:1326897/100‑1 (MQ=255)
                                                                           aTCGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTc                     <  2:2140035/100‑1 (MQ=255)
                                                                             cGTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGc                   <  1:1318736/100‑1 (MQ=255)
                                                                              gTACCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGcc                  >  2:162379/1‑100 (MQ=255)
                                                                              gTACCCGCGTATTGCCGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGcc                  >  1:5059213/1‑100 (MQ=255)
                                                                                aCCCGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGt                >  1:3520233/1‑100 (MQ=255)
                                                                                 cccGCGTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTc               >  1:4103776/1‑100 (MQ=255)
                                                                                  ccGCGTATTGCTGTGCACCTGGTCGCGAAGCGCGTTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCg              <  1:5086565/100‑1 (MQ=255)
                                                                                   cgcgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCgg             <  2:4634002/100‑1 (MQ=255)
                                                                                    gcgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGt            >  1:876014/1‑100 (MQ=255)
                                                                                     cgTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGtt           >  1:2131020/1‑100 (MQ=255)
                                                                                      gTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGttt          >  1:3113608/1‑100 (MQ=255)
                                                                                      gTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGttt          <  2:793905/100‑1 (MQ=255)
                                                                                      gTATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGttt          <  2:4303217/100‑1 (MQ=255)
                                                                                       tATTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTc         <  2:2580060/100‑1 (MQ=255)
                                                                                        aTTGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCa        <  1:4033802/100‑1 (MQ=255)
                                                                                         ttGCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCaa       <  2:3492996/100‑1 (MQ=255)
                                                                                           gCTGTGGACCTGGTCGCGCACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAAcc     >  1:4638989/1‑100 (MQ=255)
                                                                                           gCTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAAcc     >  1:86698/1‑100 (MQ=255)
                                                                                            cTGTGCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCa    >  1:1831161/1‑100 (MQ=255)
                                                                                              gtgCACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCAGc  >  2:2508715/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GAACAAATTCATCCTGCCCACGAATGCCTTGCGGGTTTTCCGGTGACGCAAGAAAGCCTTTTCGCACCATTGGAAATCGTACCCGCGTATTGCTGTGAACCTGGTCGCGAACCGCGCTCTGCAAGGAGTTTTCCATTCCTGTGGCTAACGCGCCACGCGAGCTAAACACGGTTTCGCCGTCGGTTTCAACCAGC  >  NC_000913/3716145‑3716338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: