Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,624,394 A→C I132M (ATT→ATG ytjB ← SMP_2 family putative membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,624,3940AC99.5% 701.6 / ‑5.0 199I132M (ATT→ATGytjBSMP_2 family putative membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (112/85);  total (114/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 199 total aligned reads displayed.

AATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCG  >  NC_000913/4624298‑4624490
                                                                                                |                                                                                                
    ttAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCt                                                                                           <  1:2460511/100‑1 (MQ=255)
     tAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTc                                                                                          <  1:1333840/100‑1 (MQ=255)
     tAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTc                                                                                          <  1:3432748/100‑1 (MQ=255)
      aGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCg                                                                                         <  1:3469785/100‑1 (MQ=255)
        tggtgTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGAc                                                                                       <  1:1225189/100‑1 (MQ=255)
        tggtgTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGAc                                                                                       <  2:216206/100‑1 (MQ=255)
         ngtgtTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACa                                                                                      >  1:4448918/2‑100 (MQ=255)
         ggtgTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACa                                                                                      >  2:2646141/1‑100 (MQ=255)
          gtgtTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACaa                                                                                     >  1:1114011/1‑100 (MQ=255)
          gtgtTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACaa                                                                                     <  2:3443068/100‑1 (MQ=255)
            gtTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATc                                                                                   >  2:1380759/1‑100 (MQ=255)
            gtTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATc                                                                                   >  2:2826675/1‑100 (MQ=255)
            gtTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATc                                                                                   <  1:2306454/100‑1 (MQ=255)
              tATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATctg                                                                                 >  1:4353989/1‑100 (MQ=255)
               aTCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATctgc                                                                                >  2:4015879/1‑100 (MQ=255)
               aTCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGGTTTTACCCGCCATTGGCTCGACAATctgc                                                                                >  2:3223541/1‑100 (MQ=255)
               aTCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGATTTTACCCGCCATTGGCTCGACAATctgc                                                                                >  2:5445256/1‑100 (MQ=255)
                  cACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTgg                                                                             <  1:1517637/100‑1 (MQ=255)
                   aCCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGt                                                                            <  1:2805554/100‑1 (MQ=255)
                    ccTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGTCAATCTGCTGGtt                                                                           >  2:3051067/1‑100 (MQ=255)
                     cTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTa                                                                          >  1:4721825/1‑100 (MQ=255)
                      tGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGCCAATCTGCTGGTTaa                                                                         >  2:3748508/1‑100 (MQ=255)
                      tGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTaa                                                                         <  1:2672020/100‑1 (MQ=255)
                       gTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTaaa                                                                        >  2:654049/1‑100 (MQ=255)
                         tGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAAt                                                                      <  1:3787678/100‑1 (MQ=255)
                         tGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAAt                                                                      <  1:2234540/100‑1 (MQ=255)
                         tGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACACGCCATTGGCTCGACAATCTGCTGGTTAAAAt                                                                      >  2:3674228/1‑100 (MQ=255)
                           gtcTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCTTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAg                                                                    <  2:4504063/98‑1 (MQ=255)
                            gCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAgc                                                                   >  2:2280384/1‑100 (MQ=255)
                            gCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAgc                                                                   >  1:2420775/1‑100 (MQ=255)
                             cTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAgcc                                                                  <  2:3363302/100‑1 (MQ=255)
                             cTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAgcc                                                                  >  1:2380465/1‑100 (MQ=255)
                              ttCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCTGTCCGTTTTTACCCTCCATTGGCTCGACAATCTGCTGGTTAAAATAgccg                                                                 >  2:828964/1‑100 (MQ=255)
                              ttCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAgccg                                                                 >  1:2914271/1‑100 (MQ=255)
                                cGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAgccgcc                                                               <  1:2860616/100‑1 (MQ=255)
                                 ggTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCt                                                              >  1:4074028/1‑100 (MQ=255)
                                   tGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGc                                                            <  2:1677257/100‑1 (MQ=255)
                                      cGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCttt                                                         >  1:210723/1‑100 (MQ=255)
                                       gAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCtttt                                                        <  2:1330447/100‑1 (MQ=255)
                                       gAGCGTATGGGTGTCGAGTGGCGGGCGCAGATAGCCGAGCGGTCCGGTTTTACCAGCCATTGGCTCGACAATCTGCTGGTTAACTTAGCCGCCTGCtttt                                                        >  2:1849395/1‑100 (MQ=255)
                                           gTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTAc                                                    >  1:2814455/1‑100 (MQ=255)
                                           gTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTAc                                                    >  2:1599741/1‑100 (MQ=255)
                                             aTGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCg                                                  <  1:5558595/100‑1 (MQ=255)
                                             aTGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCg                                                  <  1:905327/100‑1 (MQ=255)
                                              tGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGt                                                 >  1:5132337/1‑100 (MQ=255)
                                              tGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGt                                                 >  2:1777358/1‑100 (MQ=255)
                                               gggTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTTCTTTTTTACCGTc                                                >  2:431314/1‑100 (MQ=255)
                                                  tgtCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAg                                             <  2:1988165/100‑1 (MQ=255)
                                                   gtCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAgc                                            >  1:2509594/1‑100 (MQ=255)
                                                   ccccaGTGTCAGGCGCAGATAGCCGTGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAgc                                            <  2:4587321/96‑1 (MQ=255)
                                                    tCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAgcg                                           <  2:3953358/100‑1 (MQ=255)
                                                    tCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAgcg                                           <  2:3273577/100‑1 (MQ=255)
                                                    tCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAgcg                                           <  2:3161634/100‑1 (MQ=255)
                                                     cGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAgcgc                                          >  2:2440281/1‑100 (MQ=255)
                                                     cGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAgcgc                                          >  1:5258831/1‑100 (MQ=255)
                                                     cGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAgcgc                                          >  1:1642769/1‑100 (MQ=255)
                                                        gtgtCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGGCAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAg                                       >  2:5414426/1‑100 (MQ=255)
                                                        gtgtCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAg                                       >  1:5473030/1‑100 (MQ=255)
                                                            cAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCgg                                   >  2:4749937/1‑100 (MQ=255)
                                                              ggCGCAGATATCCGAGCGGTCCGTTTTTACCCGCGATTGGCTCGACAATCTGCAGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTc                                 >  2:5069786/1‑100 (MQ=255)
                                                              ggCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTc                                 <  2:2032061/100‑1 (MQ=255)
                                                                ggcAGATGGCCGGGCGGTCCGTTTTTCCCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTcgc                               <  2:3504479/99‑1 (MQ=255)
                                                                cgcAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTGTCGTCGAGCACCAGCCGGTcgc                               >  2:4880672/1‑100 (MQ=255)
                                                                cgcAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTcgc                               >  2:1093537/1‑100 (MQ=255)
                                                                cgcAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTcgc                               <  1:2194041/100‑1 (MQ=255)
                                                                 gcAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTAGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCTAGCGCCAGCCGGTcgcg                              >  1:3834445/1‑100 (MQ=255)
                                                                  cAGATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCgcgc                             >  1:3004043/1‑100 (MQ=255)
                                                                    gATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCAc                           >  1:1823484/1‑100 (MQ=255)
                                                                    gATAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCAc                           <  1:4892787/100‑1 (MQ=255)
                                                                    gATAGCCGAGCGGTCCGGTTTTACTCGCCATTGGCTCGCCAATCTGCTGGTTAAAATAGCCGCCTGCTTGTTTACCGTCGAGCGCCAGCCGGTCGCGCAc                           >  2:2572421/1‑100 (MQ=255)
                                                                     aTAGCCGTGGGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGTGCACt                          <  2:2182209/100‑1 (MQ=255)
                                                                     aTAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACt                          <  2:4799885/100‑1 (MQ=255)
                                                                      tAGCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACtt                         >  2:1722188/1‑100 (MQ=255)
                                                                        gCCGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCg                       >  2:3805075/1‑100 (MQ=255)
                                                                         ccGAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGa                      >  1:1080508/1‑100 (MQ=255)
                                                                           gAGCGGTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACg                    <  2:2504596/100‑1 (MQ=255)
                                                                               ggTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCttt                <  1:3320551/100‑1 (MQ=255)
                                                                               ggTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCttt                >  2:1512969/1‑100 (MQ=255)
                                                                               ggTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCttt                <  2:4491273/100‑1 (MQ=255)
                                                                               ggTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCAACGCttt                >  1:3675825/1‑100 (MQ=255)
                                                                                gTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGTTTAAAATAGCCGCCTGCTTTTTTCCCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTc               >  1:709965/1‑100 (MQ=255)
                                                                                gTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAATATAGCAGCCTGCTTTTTTACCGTCGAGCGCGAGCTGGTCGCGCACTTCGACGCTTTc               >  2:1199321/1‑100 (MQ=255)
                                                                                gTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGCGCGCCAGCCGGTCGCGCACTTCGACGCTTTc               >  1:277116/1‑100 (MQ=255)
                                                                                gTCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTc               >  2:4405123/1‑100 (MQ=255)
                                                                                 tCCGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCg              <  1:3233104/100‑1 (MQ=255)
                                                                                 tCCGTGTTTCCCCGCCATTGGGGCGACAGTCGGCTGGTTAAAATAGCGGCGTGCTTTTTTACCGTCGGGCGCCAGCCGGTCGCGCAATTCGACGCTTTCg              <  2:338066/100‑1 (MQ=255)
                                                                                  ccGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGc             <  1:4005755/100‑1 (MQ=255)
                                                                                  ccGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGc             >  1:4788923/1‑100 (MQ=255)
                                                                                  ccGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGc             >  2:1430533/1‑100 (MQ=255)
                                                                                   cGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGcc            <  1:4256199/100‑1 (MQ=255)
                                                                                   cGTTTTTACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGcc            >  2:3950000/1‑100 (MQ=255)
                                                                                    gTTTTTACAGGCCATTGGCTCGAAAATCTGCTGGGTAAAATAGCCGCGTGCGATTTTACCGTCGAGCGCAAGGCGGCCGCGCACTTCGACGCCTTCGccc           >  1:5306607/1‑99 (MQ=255)
                                                                                         tACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCCGAACg      >  2:5014053/1‑100 (MQ=255)
                                                                                         tACCCGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACg      <  2:2886777/100‑1 (MQ=255)
                                                                                           cccGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTg    >  1:2158601/1‑100 (MQ=255)
                                                                                           cccGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTg    >  1:4849736/1‑100 (MQ=255)
                                                                                           cccGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTg    >  1:3145518/1‑100 (MQ=255)
                                                                                           cccGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTg    >  1:4030329/1‑100 (MQ=255)
                                                                                            ccGCCATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGc   <  2:343517/100‑1 (MQ=255)
                                                                                            ccGCCATTGGCTCGACAATCTGCAGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGGTTTCGCCAGAACGTGc   >  2:4597868/1‑100 (MQ=255)
                                                                                                |                                                                                                
AATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCG  >  NC_000913/4624298‑4624490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: