Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 76,539 T→G E254A (GAA→GCA)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091376,5390TG98.5% 679.2 / NA 199E254A (GAA→GCA) sgrRtranscriptional DNA‑binding transcriptional activator of sgrS sRNA
Reads supporting (aligned to +/- strand):  ref base T (1/2);  major base G (100/93);  minor base A (0/3);  total (101/98)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

GAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCG  >  NC_000913/76442‑76635
                                                                                                 |                                                                                                
gAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGcg                                                                                                >  1:5542619/1‑100 (MQ=255)
gAATGCCTGAATTTTCAGTTGATTGGGGCTGTTGCGAATCACCGCATACAGACCGGTGCCGATGGGATGGCTGGAAAAGTTACTCAGGGTTTCCCATAcg                                                                                                <  2:3942753/100‑1 (MQ=255)
 aaTGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATgcgc                                                                                               >  2:1636717/1‑100 (MQ=255)
  aTGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGcgcg                                                                                              <  2:2890205/100‑1 (MQ=255)
   tGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATgcgcgc                                                                                             >  2:2772822/1‑100 (MQ=255)
     ccTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCgg                                                                                           >  1:1096853/1‑100 (MQ=255)
     ccTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCgg                                                                                           >  1:1724147/1‑100 (MQ=255)
      cTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGc                                                                                          >  2:1926112/1‑100 (MQ=255)
      cTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGc                                                                                          >  1:4682760/1‑100 (MQ=255)
      cTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGc                                                                                          <  1:3702516/100‑1 (MQ=255)
       tGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCa                                                                                         >  1:1607959/1‑100 (MQ=255)
       tGAAGTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCa                                                                                         >  2:4671896/1‑100 (MQ=255)
        gAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAg                                                                                        <  2:3615041/100‑1 (MQ=255)
        gAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAg                                                                                        <  1:1758509/100‑1 (MQ=255)
         aaTTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAgg                                                                                       <  1:5163536/100‑1 (MQ=255)
          aTTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGa                                                                                      >  2:815053/1‑100 (MQ=255)
          aTTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGa                                                                                      >  2:4468064/1‑100 (MQ=255)
           ttttCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGat                                                                                     <  1:4200197/100‑1 (MQ=255)
           ttttCAGTTGATTGGTGCTGTTGCGAAGCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGat                                                                                     <  1:4834719/100‑1 (MQ=255)
            tttCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGatc                                                                                    >  2:4298111/1‑100 (MQ=255)
                aGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCg                                                                                <  2:5425038/100‑1 (MQ=255)
                 gTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGc                                                                               >  1:1014412/1‑100 (MQ=255)
                  ttGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGcc                                                                              <  1:4384278/100‑1 (MQ=255)
                   tGATTGGTGCTGTCGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCg                                                                             <  1:1212425/100‑1 (MQ=255)
                    gATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCgg                                                                            >  1:3338526/1‑100 (MQ=255)
                    gATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCgg                                                                            <  2:71509/100‑1 (MQ=255)
                    gATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCgg                                                                            <  2:2855691/100‑1 (MQ=255)
                     aTTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGa                                                                           <  2:2861492/100‑1 (MQ=255)
                        ggTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACt                                                                        >  2:2358678/1‑100 (MQ=255)
                         gTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACtt                                                                       >  2:3787072/1‑100 (MQ=255)
                         gTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACtt                                                                       >  1:5216125/1‑100 (MQ=255)
                           gCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGc                                                                     <  2:2067707/100‑1 (MQ=255)
                            cTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGcc                                                                    >  2:1006259/1‑100 (MQ=255)
                            cTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGcc                                                                    <  2:4543593/100‑1 (MQ=255)
                             tGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGccc                                                                   <  2:282088/100‑1 (MQ=255)
                              gTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGcccc                                                                  >  2:4637397/1‑100 (MQ=255)
                              gTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGcccc                                                                  <  2:1989922/100‑1 (MQ=255)
                                tGCGAATCACAGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGGGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCcag                                                                >  2:5081873/1‑100 (MQ=255)
                                tGCGAACCACCGCATACGGACTGGTGCCGATGGGATGGCTGGCAAAGTTACTGACGGTATCCCATGCGCGCGGCAGGATCATCGGCGGAACTTGCCCcag                                                                >  2:5237981/1‑100 (MQ=255)
                                 gCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCcagc                                                               <  1:4571111/100‑1 (MQ=255)
                                 gCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCcagc                                                               <  1:3643292/100‑1 (MQ=255)
                                  cGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCcagca                                                              >  1:5237561/1‑100 (MQ=255)
                                  cGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCcagca                                                              <  2:1161197/100‑1 (MQ=255)
                                   gAATCACCGCATACGGACCGGTGCCGATGAGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCcagcag                                                             <  1:5076560/100‑1 (MQ=255)
                                   gAATCACCGCATACGGACCAGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCcagcag                                                             >  1:4284759/1‑100 (MQ=255)
                                    aaTCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGt                                                            <  2:3241873/100‑1 (MQ=255)
                                     aTCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTa                                                           >  2:3273883/1‑100 (MQ=255)
                                       cACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAAc                                                         <  2:4111764/100‑1 (MQ=255)
                                       cACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAAc                                                         <  2:1428451/100‑1 (MQ=255)
                                        aCCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACg                                                        >  1:4553003/1‑100 (MQ=255)
                                           gCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGACCATCGCCGGAACTTGCCCCAGCAGTAACGGTa                                                     <  2:4947221/100‑1 (MQ=255)
                                           gCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTa                                                     >  1:2162665/1‑100 (MQ=255)
                                           gCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTa                                                     >  1:4533023/1‑100 (MQ=255)
                                            cATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTaa                                                    >  2:4711304/1‑100 (MQ=255)
                                              tACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAAcc                                                  <  1:5408580/100‑1 (MQ=255)
                                               aCGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCa                                                 >  2:5057133/1‑100 (MQ=255)
                                                 ggACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGc                                               <  1:187865/100‑1 (MQ=255)
                                                    ccGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGt                                            >  1:1173465/1‑100 (MQ=255)
                                                    ccGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGt                                            >  1:2391186/1‑100 (MQ=255)
                                                      ggTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAGCTTGCCCCAGCAGTAACGGTAACCAGCGGTcc                                          >  1:2536249/1‑100 (MQ=255)
                                                      ggTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTcc                                          <  1:4895449/100‑1 (MQ=255)
                                                       gTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCg                                         <  1:2054005/100‑1 (MQ=255)
                                                         gCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGt                                       <  1:1677887/100‑1 (MQ=255)
                                                          ccGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGtt                                      >  1:3101567/1‑100 (MQ=255)
                                                          ccGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGtt                                      >  2:4926739/1‑100 (MQ=255)
                                                          ccGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGtt                                      <  1:3202318/100‑1 (MQ=255)
                                                           cGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTg                                     >  2:2734042/1‑100 (MQ=255)
                                                              tgggatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGt                                  <  1:5344987/100‑1 (MQ=255)
                                                               tggatggCTGGCAAAGTTACGGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTg                                 <  2:1773144/99‑1 (MQ=255)
                                                               gggatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTg                                 <  1:81060/100‑1 (MQ=255)
                                                               gggatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTg                                 <  2:5324153/100‑1 (MQ=255)
                                                                ggatggCTGGCAAAGTTACTGCGGGTTTCCAATGCGCGCGGCAGGCTCATCGCAGGACCTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTga                                <  2:250403/100‑1 (MQ=255)
                                                                ggatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTga                                >  1:942779/1‑100 (MQ=255)
                                                                 gatggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTgag                               <  1:2853578/100‑1 (MQ=255)
                                                                  atggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGCACTTGCCCCAGCAGCAACGGTACCCCGCGGTCCCGTTGCGTgaga                              >  2:1356055/1‑100 (MQ=255)
                                                                  atggCTGGCAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTgaga                              >  2:268219/1‑100 (MQ=255)
                                                                   tggctggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGAt                             <  2:3036337/100‑1 (MQ=255)
                                                                   tggctggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGAt                             <  1:2799651/100‑1 (MQ=255)
                                                                   gatctgtcAAAGTTACTGAGGGTTTCCCATACGCGCGGCAGGATCATCGCTGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCCTGAGAt                             <  2:5184670/97‑1 (MQ=255)
                                                                      ctggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGa                          >  2:3347722/1‑100 (MQ=255)
                                                                      ctggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGa                          >  2:1226987/1‑100 (MQ=255)
                                                                        ggcAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGagatagatc                        >  1:5256301/1‑99 (MQ=255)
                                                                          cAAAGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATc                      >  1:2461486/1‑100 (MQ=255)
                                                                            aaGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCa                    >  1:5012152/1‑100 (MQ=255)
                                                                             aGTTACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAg                   <  1:619170/100‑1 (MQ=255)
                                                                                tACTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGt                <  2:5256021/100‑1 (MQ=255)
                                                                                tACTGAGGGTTTCCCATGCCCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGGTGGATATCCAGCGt                >  2:5033814/1‑100 (MQ=255)
                                                                                tACTGAGGGCTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGAAGTCCGGTTGCGTGAGATGGATATCCAGCGt                >  1:1480648/1‑100 (MQ=255)
                                                                                  cTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTcc              >  2:790364/1‑100 (MQ=255)
                                                                                  cTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTcc              >  1:5446927/1‑100 (MQ=255)
                                                                                  cTGAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCATGAGATGGATATCCAGCGTcc              <  2:901836/100‑1 (MQ=255)
                                                                                    gAGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAg            >  2:5359911/1‑100 (MQ=255)
                                                                                     aGGGTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAgg           <  1:1398208/100‑1 (MQ=255)
                                                                                      gggTTTCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGGTGGCTATCCAGCGTCCAggg          >  2:1831937/1‑100 (MQ=255)
                                                                                          gtCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTc      <  2:430944/99‑1 (MQ=255)
                                                                                           tCCCATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCg     <  1:5377430/100‑1 (MQ=255)
                                                                                            nccATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCgg    >  1:4449040/2‑100 (MQ=255)
                                                                                            cccATGCGCGCGGCAGGATCATCGCCGGAGCTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCgg    <  2:2511218/100‑1 (MQ=255)
                                                                                            cccATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCgg    >  2:3176576/1‑100 (MQ=255)
                                                                                             ccATGCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGc   <  2:1510648/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCG  >  NC_000913/76442‑76635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: