Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 623,458 T→G L387V (TTG→GTG)  entS → enterobactin exporter, iron‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913623,4580TG99.5% 789.4 / ‑4.3 218L387V (TTG→GTG) entSenterobactin exporter, iron‑regulated
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (103/114);  total (104/114)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 231 total aligned reads displayed.

GCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCT  >  NC_000913/623361‑623554
                                                                                                 |                                                                                                
 cAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGgtgtgt                                                                                               >  1:1000466/1‑100 (MQ=255)
 cAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGgtgtgt                                                                                               >  2:1972671/1‑100 (MQ=255)
 cAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGgtgtgt                                                                                               >  1:3389065/1‑100 (MQ=255)
  aGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTgtgtt                                                                                              <  2:4345014/100‑1 (MQ=255)
   gAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTgtgttg                                                                                             >  2:2652606/1‑100 (MQ=255)
      cGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGAtt                                                                                          >  2:3160091/1‑100 (MQ=255)
       gTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTa                                                                                         <  2:679725/100‑1 (MQ=255)
        tGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTAt                                                                                        >  2:3466123/1‑100 (MQ=255)
        tGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGACCGGTTTTGGTGTGTTGATTAt                                                                                        >  1:5193457/1‑100 (MQ=255)
           cGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCgg                                                                                     <  1:2519514/100‑1 (MQ=255)
           cGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCgg                                                                                     <  1:2412127/100‑1 (MQ=255)
             ggCGATGCCATAGGCGCGGAGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGATTTTGGTGAGTTGATTATCGGCg                                                                                   >  2:5029378/1‑100 (MQ=255)
              gCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCgt                                                                                  <  1:1734839/100‑1 (MQ=255)
                 aTGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGtgtt                                                                               <  1:3025236/100‑1 (MQ=255)
                   gtcATAGGCGCGGCGCTGCTGGGTGGGTTGGGCGCGATGAGGACACCGGTTACTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGtgttgt                                                                             <  2:5464850/98‑1 (MQ=255)
                    ccATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTgttgtt                                                                            <  2:4896831/100‑1 (MQ=255)
                     cctAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGAGGTGTTGATTATCGGCGTGTTGTTa                                                                           >  2:5443768/3‑100 (MQ=255)
                     cATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTa                                                                           >  1:5551607/1‑100 (MQ=255)
                      aTAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTAt                                                                          <  2:397637/100‑1 (MQ=255)
                      aTAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTAt                                                                          >  1:2203216/1‑100 (MQ=255)
                       tAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTAtt                                                                         <  1:2746383/100‑1 (MQ=255)
                         ggcgcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTAttag                                                                       >  1:282813/1‑98 (MQ=255)
                          gcgcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgct                                                                      <  2:3365669/100‑1 (MQ=255)
                          gcgcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgct                                                                      <  2:1767198/100‑1 (MQ=255)
                          gcgcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgct                                                                      <  2:4595355/100‑1 (MQ=255)
                            gcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctgg                                                                    <  1:3659022/100‑1 (MQ=255)
                            gcggcgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctgg                                                                    >  1:1464495/1‑100 (MQ=255)
                              ggcgcTGCTGGGTGGTTTGGGCGCGATGGTGACACCGGTTGCTTCCGCAAGCGCGAGCGATTTTGGTGTGTTGATTATCGGCGTGTTGATATtgctggtg                                                                  >  1:4905945/1‑100 (MQ=255)
                                cgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATtgctggtgct                                                                >  2:2793595/1‑100 (MQ=255)
                                cgcTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGCTGTTATtgctggtgct                                                                >  2:4559374/1‑100 (MQ=255)
                                   tgctgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTgctggtgctggt                                                             <  1:2092838/100‑1 (MQ=255)
                                   tactgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTgctggtgctggt                                                             <  1:2633817/98‑1 (MQ=255)
                                    gctgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGctggtgctggtg                                                            <  1:1227814/100‑1 (MQ=255)
                                     ctgGGTGGTTGGGGCGCGATGATGACACCGGTTGCTTCCGCGAGCGCAAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCtggtgg                                                           <  2:653740/100‑1 (MQ=255)
                                      tgGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGa                                                          >  1:2473994/1‑100 (MQ=255)
                                       gggTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAg                                                         <  1:1815682/100‑1 (MQ=255)
                                        ggtggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGt                                                        <  1:3501769/100‑1 (MQ=255)
                                        ggtggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGt                                                        >  1:4362191/1‑100 (MQ=255)
                                           ggtTTGGGCGCGATGATGACACCTGTTGCTTCCGCAAGCGCGTGCGGTTTTGGTGTGTTGTTTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGc                                                     <  1:4856726/100‑1 (MQ=255)
                                           ggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGc                                                     <  1:5028390/100‑1 (MQ=255)
                                           ggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGc                                                     >  2:1417812/1‑100 (MQ=255)
                                           ggtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGc                                                     >  1:381292/1‑100 (MQ=255)
                                            gtTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCg                                                    <  1:1620578/100‑1 (MQ=255)
                                             tttGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGa                                                   >  1:3298554/1‑100 (MQ=255)
                                              ttGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGAc                                                  <  2:2845669/100‑1 (MQ=255)
                                                 ggCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACAtt                                               >  1:1178645/1‑100 (MQ=255)
                                                 ggCGCGATGATGACACCGGTTGCTGCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGGTGTTATTGCTGGTGCTGGTGGAGTTGCGACAtt                                               >  2:3625191/1‑100 (MQ=255)
                                                  gcgcGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGagatcg                                              >  2:3843699/1‑95 (MQ=255)
                                                  gcgcGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACAttt                                              <  2:1853737/100‑1 (MQ=255)
                                                  gagggattatGACTCGTGTTGCTTGCGCGAGCGGGGGTGGTTTTGGTGTGTTGGTTATCGGCGTGTTGTTATTTCTGGTGCTGGGGGAGTTGCGACAttt                                              <  2:4694270/96‑1 (MQ=255)
                                                   cgcgATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACAtttt                                             >  2:83847/1‑100 (MQ=255)
                                                    gcgATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTc                                            >  2:1172033/1‑100 (MQ=255)
                                                     cgATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCg                                           <  1:3885293/100‑1 (MQ=255)
                                                     cgATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCg                                           <  2:4737735/100‑1 (MQ=255)
                                                     cgATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCg                                           <  1:13351/100‑1 (MQ=255)
                                                      gatgatGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGc                                          <  2:3091323/100‑1 (MQ=255)
                                                      gatgatGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGc                                          <  1:1363169/100‑1 (MQ=255)
                                                       atgatgACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGcc                                         >  1:1257598/1‑100 (MQ=255)
                                                       atgatgACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGcc                                         <  2:2083758/100‑1 (MQ=255)
                                                       atgatgACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGcc                                         <  1:2866070/100‑1 (MQ=255)
                                                          atgaCACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGa                                      >  1:3476329/1‑100 (MQ=255)
                                                           tgaCACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAc                                     <  2:4205757/100‑1 (MQ=255)
                                                            gaCACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcg                                    >  2:4295681/1‑100 (MQ=255)
                                                             acacCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgc                                   <  2:2392610/100‑1 (MQ=255)
                                                              cacCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgcc                                  >  2:394369/1‑100 (MQ=255)
                                                              cacCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgcc                                  >  1:3150052/1‑100 (MQ=255)
                                                               acCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgccg                                 <  1:3618038/100‑1 (MQ=255)
                                                                  ggTTGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGAcgccgccg                              >  1:3753542/1‑100 (MQ=255)
                                                                     tGCTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAg                           <  1:5244554/100‑1 (MQ=255)
                                                                     tGCTTCCGCAAGCGCGAGCGGATTTGGTGTGTTGATTATCGGCGTGATGTTATTGCTGGTGCTGGTGGCGTTGCGACATTTTCGCCAGACGCAGCCGCAg                           >  2:5106985/1‑100 (MQ=255)
                                                                       cTTCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGt                         >  2:3339791/1‑100 (MQ=255)
                                                                        ttCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTg                        <  2:1500649/100‑1 (MQ=255)
                                                                         tCCGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGAGCGAGTGGATTTGCGATATTTTCGCCAGACGCCGCCGCAGGTGa                       >  2:5249547/1‑100 (MQ=255)
                                                                          ccGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGAc                      >  2:493340/1‑100 (MQ=255)
                                                                           cGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACa                     >  1:4965914/1‑100 (MQ=255)
                                                                           cGCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACa                     >  2:5413503/1‑100 (MQ=255)
                                                                            gCAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAg                    <  2:1339346/100‑1 (MQ=255)
                                                                             cAAGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGc                   <  1:4764655/100‑1 (MQ=255)
                                                                              aaGCGCGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCg                  >  1:778149/1‑100 (MQ=255)
                                                                                gcgcGAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTc                <  2:124463/100‑1 (MQ=255)
                                                                                 cgcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACTCTGCCGCAGGTGACAGCGTcc               <  1:4751748/100‑1 (MQ=255)
                                                                                 cgcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTcc               <  2:3548255/100‑1 (MQ=255)
                                                                                 cgcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTcc               <  1:3962177/100‑1 (MQ=255)
                                                                                 cgcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTcc               <  1:909650/100‑1 (MQ=255)
                                                                                 cgcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTcc               <  2:1577853/100‑1 (MQ=255)
                                                                                  gcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCg              <  1:4234662/100‑1 (MQ=255)
                                                                                  gcgAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCg              <  1:4561724/100‑1 (MQ=255)
                                                                                    gAGCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGAc            <  1:4599759/100‑1 (MQ=255)
                                                                                    gAGCGGTTTGGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCACGTGACAGCGTCCGAc            <  2:4186973/100‑1 (MQ=255)
                                                                                      gCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAg          <  2:1145558/100‑1 (MQ=255)
                                                                                      gCGGTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAg          <  2:2554281/100‑1 (MQ=255)
                                                                                         gTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTa       <  1:4692972/100‑1 (MQ=255)
                                                                                         gTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTa       >  1:4523846/1‑100 (MQ=255)
                                                                                         gTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTa       <  1:5089621/100‑1 (MQ=255)
                                                                                         gTTTTGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTa       >  1:3361387/1‑100 (MQ=255)
                                                                                          ttttGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTaa      <  2:3719088/100‑1 (MQ=255)
                                                                                           tttGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAAt     <  2:3282256/100‑1 (MQ=255)
                                                                                           tttGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAAt     <  1:3189588/100‑1 (MQ=255)
                                                                                           tttGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAAt     <  1:1593016/100‑1 (MQ=255)
                                                                                             tGGTGTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGc   >  2:1811874/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GCAGAACGTGACGGGCGATGCCATAGGCGCGGCGCTGCTGGGTGGTTTGGGCGCGATGATGACACCGGTTGCTTCCGCAAGCGCGAGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCT  >  NC_000913/623361‑623554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: