Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 626,842 A→C D258A (GAT→GCT)  entE → 2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913626,8420AC99.1% 828.9 / ‑2.3 229D258A (GAT→GCT) entE2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base C (120/106);  total (123/106)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 230 total aligned reads displayed.

ACCTGTGCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAT  >  NC_000913/626746‑626941
                                                                                                |                                                                                                   
 ccTGTGCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTccc                                                                                                 >  1:3485231/1‑100 (MQ=255)
    gtgCGCGATCCCGGCTGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAgc                                                                                              <  2:4610006/100‑1 (MQ=255)
    gtgCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAgc                                                                                              <  2:4848287/100‑1 (MQ=255)
    gtgCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAgc                                                                                              >  2:1548782/1‑100 (MQ=255)
     tgCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAgcg                                                                                             <  2:794445/100‑1 (MQ=255)
       cgcgATCCCGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGcc                                                                                           >  1:996185/1‑100 (MQ=255)
       cgcgATCCCGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGcc                                                                                           <  2:3567047/100‑1 (MQ=255)
       cgcgATCCCGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGcc                                                                                           >  1:3112674/1‑100 (MQ=255)
       cgcgATCCCGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGcc                                                                                           >  2:3181409/1‑100 (MQ=255)
             cccGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGctc                                                                                     >  1:5306644/1‑100 (MQ=255)
             cccGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGctc                                                                                     >  1:3350651/1‑100 (MQ=255)
               cggcggCTGATAACTACGCCATGAGTTTGCCAGGCTCGCTTGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGACCGCTCCCAGCGCCACGCTCTg                                                                                   <  2:5254651/100‑1 (MQ=255)
                  cggcTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGttt                                                                                <  2:3120371/100‑1 (MQ=255)
                     cTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTccc                                                                             >  1:21918/1‑100 (MQ=255)
                     cTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTccc                                                                             <  2:3459336/100‑1 (MQ=255)
                     cTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTccc                                                                             <  2:4469693/100‑1 (MQ=255)
                         tAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattg                                                                         <  2:2537431/100‑1 (MQ=255)
                         tAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattg                                                                         >  1:2973160/1‑100 (MQ=255)
                         tAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattg                                                                         >  1:5484151/1‑100 (MQ=255)
                          aaCTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattga                                                                        <  2:2836837/100‑1 (MQ=255)
                          aaCTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattga                                                                        <  2:4097006/100‑1 (MQ=255)
                          aaCTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattga                                                                        >  1:1393718/1‑100 (MQ=255)
                           aCTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattgat                                                                       <  2:2240021/100‑1 (MQ=255)
                           aCTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattgat                                                                       <  1:3062368/100‑1 (MQ=255)
                            cTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattgatt                                                                      >  1:1043286/1‑100 (MQ=255)
                             tACGCCATGAGTTCTCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattgattg                                                                     <  2:3776575/100‑1 (MQ=255)
                             tACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCattgattg                                                                     >  1:1139782/1‑100 (MQ=255)
                              aCGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCAttgattga                                                                    >  2:1745349/1‑100 (MQ=255)
                               cGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGaa                                                                   <  1:3753054/100‑1 (MQ=255)
                               cGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGaa                                                                   >  1:100414/1‑100 (MQ=255)
                                gCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGaaa                                                                  >  1:4368924/1‑100 (MQ=255)
                                  cATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGaaaaa                                                                <  1:876894/100‑1 (MQ=255)
                                      aGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATc                                                            >  1:2537948/1‑100 (MQ=255)
                                       gTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCa                                                           >  2:1815635/1‑100 (MQ=255)
                                       gTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCa                                                           >  1:619548/1‑100 (MQ=255)
                                       gTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCa                                                           >  2:3528363/1‑100 (MQ=255)
                                       gTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCa                                                           >  2:957044/1‑100 (MQ=255)
                                        ttCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAg                                                          <  1:5137970/100‑1 (MQ=255)
                                        gccGCCGGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAg                                                          <  2:1271253/98‑1 (MQ=255)
                                         tCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCAAAAAAATAAAAAACATCAgg                                                         <  2:208114/100‑1 (MQ=255)
                                          ctccAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGt                                                        <  1:1144029/98‑1 (MQ=255)
                                          cGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGt                                                        <  2:4880087/100‑1 (MQ=255)
                                          cGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGt                                                        <  2:335076/100‑1 (MQ=255)
                                          cGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGt                                                        >  1:4381450/1‑100 (MQ=255)
                                          cGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGt                                                        <  1:4298605/100‑1 (MQ=255)
                                          cGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGt                                                        >  1:3380110/1‑100 (MQ=255)
                                          cGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGt                                                        <  2:2567403/100‑1 (MQ=255)
                                           gCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGtt                                                       <  2:3945546/100‑1 (MQ=255)
                                           gCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGtt                                                       <  1:836015/100‑1 (MQ=255)
                                            ccAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGtag                                                      >  2:1128831/1‑98 (MQ=255)
                                               ggATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACg                                                   >  1:1453952/1‑100 (MQ=255)
                                               ggATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACg                                                   >  2:2132746/1‑100 (MQ=255)
                                               ggATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACg                                                   <  1:1215548/100‑1 (MQ=255)
                                               ggATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGCTAACg                                                   >  2:5209476/1‑100 (MQ=255)
                                               ggATCGCTGGGCGTCTTCCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACg                                                   >  2:967421/1‑100 (MQ=255)
                                                gATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGt                                                  >  2:3685748/1‑100 (MQ=255)
                                                gATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGt                                                  >  1:2634249/1‑100 (MQ=255)
                                                  tCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTa                                                <  1:3203699/100‑1 (MQ=255)
                                                   cGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTAc                                               >  2:1993640/1‑100 (MQ=255)
                                                    tttGGGAGTCTTTTTTGCCGGAGCAACGGTTGTTCTGGCGGATGTTTCCAGCGCCACGTTCTGGTTCCAATGGATTGAAAAGCATCAGGTTAATTTTAcc                                              >  2:17092/3‑100 (MQ=255)
                                                    gCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTAcc                                              >  1:3760010/1‑100 (MQ=255)
                                                     cTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACcg                                             <  2:2390602/100‑1 (MQ=255)
                                                     cTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACcg                                             >  2:2819536/1‑100 (MQ=255)
                                                     cTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACcg                                             <  2:596168/100‑1 (MQ=255)
                                                          ngTCTTTCTTGCCGGAGGGACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTg                                        >  2:657081/2‑100 (MQ=255)
                                                          cGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTg                                        >  1:487428/1‑100 (MQ=255)
                                                          cGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTg                                        <  1:2509234/100‑1 (MQ=255)
                                                          cGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTg                                        >  1:2145715/1‑100 (MQ=255)
                                                           gTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTgg                                       <  1:1869869/100‑1 (MQ=255)
                                                             ctttcttGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTg                                     <  1:3823032/100‑1 (MQ=255)
                                                             ctttcttGCCGGAGGAACGATTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACATTACCGCGCTGGTg                                     >  2:5027990/1‑100 (MQ=255)
                                                              tttcttGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGc                                    <  2:4341907/100‑1 (MQ=255)
                                                                tcttGCCGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCa                                  <  2:378412/100‑1 (MQ=255)
                                                                      cGGAGGAACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCa                            <  2:836517/100‑1 (MQ=255)
                                                                       ggaggaACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAg                           >  1:4637768/1‑100 (MQ=255)
                                                                       ggaggaACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAg                           >  2:1725120/1‑100 (MQ=255)
                                                                       ggaggaACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAg                           >  1:4232482/1‑100 (MQ=255)
                                                                       ggaggaACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAg                           <  1:2790105/100‑1 (MQ=255)
                                                                       ggaggaACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAg                           >  2:3390587/1‑100 (MQ=255)
                                                                        gaggaACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAgn                          <  1:4413620/100‑2 (MQ=255)
                                                                          ggaACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCa                        >  2:4089357/1‑100 (MQ=255)
                                                                          ggaACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCa                        <  1:3949206/100‑1 (MQ=255)
                                                                           gaACGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAg                       <  2:3718828/100‑1 (MQ=255)
                                                                            aaCGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGc                      >  1:3396352/1‑100 (MQ=255)
                                                                             aCGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGcc                     >  1:3803542/1‑100 (MQ=255)
                                                                             aCGGTTGTTCTGGCGGCCGCTCCCAGCGCCACGCTCTATTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGcc                     <  2:3662208/100‑1 (MQ=255)
                                                                                  tgttCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTgg                >  2:343404/1‑100 (MQ=255)
                                                                                   gttCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGt               >  1:5070/1‑100 (MQ=255)
                                                                                   gttCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGt               >  2:2320635/1‑100 (MQ=255)
                                                                                     tCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTg             <  1:3561289/100‑1 (MQ=255)
                                                                                     tCTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTg             <  2:549438/100‑1 (MQ=255)
                                                                                      cTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGc            <  2:2945268/100‑1 (MQ=255)
                                                                                      cTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGc            <  2:630194/100‑1 (MQ=255)
                                                                                      cTGGCGGCCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGc            <  2:2608577/100‑1 (MQ=255)
                                                                                         gcggcCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAgg         <  1:1751148/100‑1 (MQ=255)
                                                                                          cggcCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGgc        >  1:4492988/1‑100 (MQ=255)
                                                                                           ggcCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGgcg       <  2:3031023/100‑1 (MQ=255)
                                                                                           ggcCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGgcg       <  1:3000983/100‑1 (MQ=255)
                                                                                            gcCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGgcgc      <  1:735601/100‑1 (MQ=255)
                                                                                            gcCGCTCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGgcgc      <  1:4903364/100‑1 (MQ=255)
                                                                                                |                                                                                                   
ACCTGTGCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCGCCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAT  >  NC_000913/626746‑626941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑