BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,479,936	A→C	N267H (AAC→CAC)	ynbC →	putative esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,479,936	0	A	C	99.0%	672.6 / ‑4.5	192	N267H (AAC→CAC)	ynbC	putative esterase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (106/84); total (107/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

TCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAG  >  NC_000913/1479839‑1480032

 cGCAAACCGCAGATTGATTTTTATCAGAGAGTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGcaca                                                                                               <  1:1607957/100‑1 (MQ=255)

  gCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAg                                                                                              >  2:3415675/1‑100 (MQ=255)

     aaCCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGgc                                                                                           >  1:428900/1‑100 (MQ=255)

     aaCCGCAGATAGATTTTTATCAGAGATTACGTAGGCCTCTGAAAGAGCTGCATGTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGgc                                                                                           >  2:5076127/1‑100 (MQ=255)

      aCCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGgcg                                                                                          >  1:4236353/1‑100 (MQ=255)

      aCCGCAGAGTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATATGCTGCCAGGCTTTTATCACGACCCGTTGGGTGAAGAGCACAGGgcg                                                                                          >  2:2641454/1‑100 (MQ=255)

       ccGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGgcgc                                                                                         >  2:1486724/1‑100 (MQ=255)

       ccGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGgcgc                                                                                         <  2:3303731/100‑1 (MQ=255)

        cGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCt                                                                                        >  2:2450789/1‑100 (MQ=255)

         gCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTg                                                                                       >  1:2164582/1‑100 (MQ=255)

         gCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTg                                                                                       <  1:2485773/100‑1 (MQ=255)

         gCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTg                                                                                       <  2:354435/100‑1 (MQ=255)

          cAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTgg                                                                                      <  1:2933569/100‑1 (MQ=255)

            gattgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCa                                                                                    <  2:2141964/100‑1 (MQ=255)

            gattgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCa                                                                                    >  1:692416/1‑100 (MQ=255)

            gattgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCa                                                                                    >  1:2105668/1‑100 (MQ=255)

              ttgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCAtt                                                                                  <  1:1550660/100‑1 (MQ=255)

               tgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCAttt                                                                                 <  2:2492123/100‑1 (MQ=255)

               tgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCAttt                                                                                 >  2:1740286/1‑100 (MQ=255)

               tgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCAttt                                                                                 >  2:141442/1‑100 (MQ=255)

               tgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCAttt                                                                                 <  2:5163055/100‑1 (MQ=255)

               tgattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGAAttt                                                                                 >  1:5560483/1‑100 (MQ=255)

                gattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTg                                                                                >  2:2900626/1‑100 (MQ=255)

                gattTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTg                                                                                <  1:2564309/100‑1 (MQ=255)

                 attTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGa                                                                               >  2:3779707/1‑100 (MQ=255)

                  tttttATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGag                                                                              >  1:877889/1‑99 (MQ=255)

                  tttttATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGaa                                                                              <  2:3150297/100‑1 (MQ=255)

                  tttttATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGaa                                                                              >  2:2077888/1‑100 (MQ=255)

                  tttttATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGaa                                                                              >  1:2015227/1‑100 (MQ=255)

                   ttttATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGaaa                                                                             >  2:2156418/1‑100 (MQ=255)

                    tttATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGaaaa                                                                            >  2:20262/1‑100 (MQ=255)

                    tttATCAGAAATTACGTAGCCCTCTGAAAGCGCTGCAACTGCTCACAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGaaaa                                                                            <  2:5293398/100‑1 (MQ=255)

                     ttATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGaaaaa                                                                           <  2:3493544/100‑1 (MQ=255)

                       aTCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAAt                                                                         >  1:416662/1‑100 (MQ=255)

                        tCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATg                                                                        <  2:4610284/100‑1 (MQ=255)

                         cAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGc                                                                       <  1:5166475/100‑1 (MQ=255)

                         cAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGc                                                                       <  1:3109349/100‑1 (MQ=255)

                         cAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGc                                                                       >  2:4820593/1‑100 (MQ=255)

                          agagATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCa                                                                      <  1:1654872/100‑1 (MQ=255)

                           gagaTTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCaa                                                                     >  1:1405307/1‑100 (MQ=255)

                            agaTTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCaaa                                                                    <  2:877092/100‑1 (MQ=255)

                              aTTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGc                                                                  <  1:4482500/100‑1 (MQ=255)

                                        ccTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTc                                                        >  1:767865/1‑100 (MQ=255)

                                        ccTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTc                                                        >  1:4832361/1‑100 (MQ=255)

                                        ccTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTc                                                        <  1:2179097/100‑1 (MQ=255)

                                         ctctGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCg                                                       <  1:4519297/100‑1 (MQ=255)

                                         ctctGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCg                                                       <  2:666702/100‑1 (MQ=255)

                                          tctGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGt                                                      >  2:3982897/1‑100 (MQ=255)

                                           ctGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAACATGCAAAGCTGTACTAGTCGtt                                                     >  2:4386532/1‑100 (MQ=255)

                                           ctGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGtt                                                     <  1:4049826/100‑1 (MQ=255)

                                             gAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTta                                                   <  1:466943/100‑1 (MQ=255)

                                             gAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTta                                                   >  2:4952922/1‑100 (MQ=255)

                                               aaGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGAATATGAAAAAATGCAAAGCTTTATTAGTCGTTtata                                                 <  2:5436133/100‑1 (MQ=255)

                                                agagCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTatat                                                <  1:3616893/100‑1 (MQ=255)

                                                   gCTGCATCTGCTGCCAGGCTTTTATCACGAAACGTTGGGTGAAGAGCACAGGGAGAAAAAAAAAAAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCt                                             <  2:1841409/100‑1 (MQ=255)

                                                    cTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTa                                            <  2:2371679/100‑1 (MQ=255)

                                                    cTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGAAAAAATAAGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTa                                            <  2:4764118/100‑1 (MQ=255)

                                                     tGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTaa                                           <  1:2691240/100‑1 (MQ=255)

                                                     tGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTaa                                           >  1:3486268/1‑100 (MQ=255)

                                                      gCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAAc                                          <  2:4265615/100‑1 (MQ=255)

                                                      gCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAAc                                          <  2:859322/100‑1 (MQ=255)

                                                       cATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACa                                         <  1:1278478/100‑1 (MQ=255)

                                                        aTCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACaa                                        >  2:803577/1‑100 (MQ=255)

                                                         tCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACaaa                                       <  1:3474820/100‑1 (MQ=255)

                                                           tgctgcCAGGCTTTTATCACGCCACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATc                                     >  1:4757682/1‑100 (MQ=255)

                                                           tgctgcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATc                                     >  1:512073/1‑100 (MQ=255)

                                                             ctgcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTCATTAGTCGTTTATATGCTAACAAATCGc                                   >  1:5070824/1‑100 (MQ=255)

                                                               gcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaa                                 >  1:2414368/1‑100 (MQ=255)

                                                               gcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaa                                 >  2:347118/1‑100 (MQ=255)

                                                               gcCAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaa                                 >  1:3311231/1‑100 (MQ=255)

                                                                ccAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaaa                                <  1:1153249/100‑1 (MQ=255)

                                                                ccAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaaa                                >  2:3337260/1‑100 (MQ=255)

                                                                 cAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAGTCGCaaaa                               >  1:5469595/1‑100 (MQ=255)

                                                                 cAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaaaa                               >  2:2009754/1‑100 (MQ=255)

                                                                 cAGGCTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCaaaa                               >  2:3387809/1‑100 (MQ=255)

                                                                     cTTTTATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAAttt                           <  1:3814693/100‑1 (MQ=255)

                                                                      ttttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTg                          <  1:3428960/100‑1 (MQ=255)

                                                                      ttttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTg                          <  1:1836435/100‑1 (MQ=255)

                                                                      ttttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTg                          >  1:1014053/1‑100 (MQ=255)

                                                                       tttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGa                         <  1:362564/100‑1 (MQ=255)

                                                                       tttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGa                         >  1:261374/1‑100 (MQ=255)

                                                                       tttATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGa                         <  2:2536760/100‑1 (MQ=255)

                                                                         tATCACGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGAtt                       <  2:1974136/100‑1 (MQ=255)

                                                                             aCGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCa                   >  1:4369907/1‑100 (MQ=255)

                                                                              cGACACGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAg                  <  2:2776031/100‑1 (MQ=255)

                                                                                 cacGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCAt               <  2:258813/100‑1 (MQ=255)

                                                                                 cacGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAAGGCAAAGCATTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATGAGCAt               >  1:5275876/1‑100 (MQ=255)

                                                                                 cacGTGAGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCAt               <  2:5496873/100‑1 (MQ=255)

                                                                                  acGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATg              <  1:4929224/100‑1 (MQ=255)

                                                                                  acGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATg              >  1:5343631/1‑100 (MQ=255)

                                                                                   cGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGa             >  1:4693075/1‑100 (MQ=255)

                                                                                   cGTTGGGTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGa             >  2:1119703/1‑100 (MQ=255)

                                                                                       gggTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGAc         >  1:4939950/1‑100 (MQ=255)

                                                                                       gggTGAAGAGCACAGGCCGCTGGCATCTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGAc         >  1:3891500/1‑100 (MQ=255)

                                                                                       aggTGAAGAGAACAGGGCGCTGGCATCTGAAAAAATGTAAAGCTTTATTAGTCTTTTATGTGATAAAAAAAAAAAAAAATTTGATTATCAGCATGAAGAc         <  2:1055454/99‑1 (MQ=255)

                                                                                        ggTGAAGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACt        <  2:3091063/100‑1 (MQ=255)

                                                                                            aaGAGCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGcac    <  1:2898487/100‑1 (MQ=255)

                                                                                             agagCATAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTCTATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGcaca   >  2:3242683/1‑100 (MQ=255)

                                                                                             agagCACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGcaca   >  1:1827347/1‑100 (MQ=255)

                                                                                              gagcACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAg  <  2:4197791/100‑1 (MQ=255)

TCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAG  >  NC_000913/1479839‑1480032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`