Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,738,276 A→C M145L (ATG→CTG)  purR → transcriptional repressor, hypoxanthine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,738,2760AC99.1% 786.4 / ‑4.2 221M145L (ATG→CTG) purRtranscriptional repressor, hypoxanthine‑binding
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (119/100);  total (120/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 221 total aligned reads displayed.

GCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCG  >  NC_000913/1738179‑1738372
                                                                                                 |                                                                                                
gcAAAATCGCGTCGATGGTCTGCTGGTGATGTGTTGTGTGTTCCTAGAGTCTTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTg                                                                                                <  2:520763/100‑1 (MQ=255)
 cAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTgg                                                                                               <  1:673781/100‑1 (MQ=255)
 cAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTgg                                                                                               >  1:3582691/1‑100 (MQ=255)
 cAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTgg                                                                                               <  1:4396293/100‑1 (MQ=255)
   aaaaCGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGAc                                                                                             <  1:1422521/100‑1 (MQ=255)
   aaaaCGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGAc                                                                                             <  1:4541398/100‑1 (MQ=255)
    aaaCGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACt                                                                                            >  1:4638915/1‑100 (MQ=255)
     aaCGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACtn                                                                                           <  1:4445217/100‑2 (MQ=255)
     aaCGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTg                                                                                           >  1:3688201/1‑100 (MQ=255)
      aCGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTgg                                                                                          <  2:2069558/100‑1 (MQ=255)
      aCGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTgg                                                                                          <  1:3030496/100‑1 (MQ=255)
      aCGCGTCGATGCTCTGCTGGTGATGTGTTCTGAGTACCCACAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTgg                                                                                          <  1:4919424/100‑1 (MQ=255)
        gcgTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTgggg                                                                                        <  1:4348487/100‑1 (MQ=255)
        gcgTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTgggg                                                                                        <  1:274462/100‑1 (MQ=255)
         cgTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGt                                                                                       >  2:4742552/1‑100 (MQ=255)
             gATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAg                                                                                   >  2:1306475/1‑100 (MQ=255)
                 gTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCaaa                                                                               >  1:1107470/1‑100 (MQ=255)
                 gTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCaaa                                                                               >  1:3378134/1‑100 (MQ=255)
                   ctgctgGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAg                                                                             >  1:872081/1‑100 (MQ=255)
                    atctgGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGc                                                                            <  1:3259375/98‑1 (MQ=255)
                      ctgGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATTGTGGTCCTGGACTGGGGTGAAGCAAAAGCTg                                                                          <  1:4464846/100‑1 (MQ=255)
                      ctgGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTg                                                                          <  2:3703116/100‑1 (MQ=255)
                      ctgGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTg                                                                          >  1:3127957/1‑100 (MQ=255)
                       tgGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGccgc                                                                         <  1:4604495/100‑4 (MQ=255)
                        ggTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGcagat                                                                        >  2:3229032/1‑96 (MQ=255)
                        ggTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGAc                                                                        <  2:3390921/100‑1 (MQ=255)
                        ggTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGAc                                                                        <  1:5380476/100‑1 (MQ=255)
                         gTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACt                                                                       <  2:4683372/100‑1 (MQ=255)
                           gATGTGTTCTGAGTACCCAGATCCGTTGCTGGCGATGCGTGAAGAGTATCGCCATATCCCAATGGTTGTCCTGGACTGTTGTGAAGCTAAAGCTGACTTc                                                                     >  2:4411338/1‑100 (MQ=255)
                           gATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGCCTGGGGTGAAGCAAAAGCTGACTTc                                                                     >  1:2794222/1‑100 (MQ=255)
                           gATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTc                                                                     >  2:1250607/1‑100 (MQ=255)
                           gATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTc                                                                     <  2:5436968/100‑1 (MQ=255)
                             tgtgTTCTGGGTACCCAGGGCCGTTGCTGGCGATGCGGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCAc                                                                   <  2:2016174/100‑1 (MQ=255)
                             tgtgTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCAc                                                                   <  2:1633559/100‑1 (MQ=255)
                              gtgtTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCAcc                                                                  <  1:1267432/100‑1 (MQ=255)
                               tgtTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGATGGTCCGGGAGTGGGGTGGAGCAAGAGCTGACTTCACCg                                                                 >  1:1381287/1‑100 (MQ=255)
                                gtTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGa                                                                >  1:3889658/1‑100 (MQ=255)
                                gtTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGa                                                                >  1:5096078/1‑100 (MQ=255)
                                gtTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGa                                                                >  2:4515790/1‑100 (MQ=255)
                                gtTCGGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGa                                                                >  2:1914059/1‑100 (MQ=255)
                                 ttCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGAt                                                               <  1:2507487/100‑1 (MQ=255)
                                  tCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATg                                                              >  2:2828171/1‑100 (MQ=255)
                                   cTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAGAAGCTGACTTCACCGATGc                                                             >  2:4917698/1‑100 (MQ=255)
                                   cTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCAGATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCGCCGATGc                                                             >  2:5113573/1‑100 (MQ=255)
                                    tGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCg                                                            >  2:74105/1‑100 (MQ=255)
                                     gAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCgg                                                           >  1:1646057/1‑100 (MQ=255)
                                         aCCCAGAGCCGTTGCTGGCGATGCTGGAATAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCAt                                                       <  1:3059404/100‑1 (MQ=255)
                                         aCCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCan                                                       <  1:847293/100‑2 (MQ=255)
                                         aCCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCAt                                                       >  2:1826691/1‑100 (MQ=255)
                                         aCCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCAt                                                       >  1:3956212/1‑100 (MQ=255)
                                         aCCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCAt                                                       >  2:2607135/1‑100 (MQ=255)
                                          cccAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCAtt                                                      <  2:888788/100‑1 (MQ=255)
                                          cccAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCAtt                                                      >  1:4022742/1‑100 (MQ=255)
                                           ccAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTg                                                     >  2:3319869/1‑100 (MQ=255)
                                           ccAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTg                                                     >  1:4113196/1‑100 (MQ=255)
                                            cAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGa                                                    <  1:1564458/100‑1 (MQ=255)
                                            cAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGa                                                    >  1:917094/1‑100 (MQ=255)
                                             agagCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGAt                                                   >  1:1195542/1‑100 (MQ=255)
                                              gagCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATa                                                  >  1:2909983/1‑100 (MQ=255)
                                                gCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCGCTGGTGGTACGGGTCTGGAGTGAAGCAAAAGCTGACTTCTCCGATGCGGTCATTGATAAc                                                >  2:5341969/1‑100 (MQ=255)
                                                 ccGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAAcg                                               >  2:3080966/1‑100 (MQ=255)
                                                   gTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAAcgcg                                             <  1:484294/100‑1 (MQ=255)
                                                   gTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAAcgcg                                             >  1:2241041/1‑100 (MQ=255)
                                                   gTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAAcgcg                                             >  2:2241071/1‑100 (MQ=255)
                                                   gTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTCAAGCAAAAGCTGACTTCACCGGTGCGGTCATTGATAAcgcg                                             <  2:815045/100‑1 (MQ=255)
                                                     tGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGtt                                           >  1:3521988/1‑100 (MQ=255)
                                                      gCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTc                                          <  2:2421338/100‑1 (MQ=255)
                                                       cTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCg                                         <  2:4075977/100‑1 (MQ=255)
                                                        tGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGa                                        <  2:5025692/100‑1 (MQ=255)
                                                        tGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGa                                        >  2:2834978/1‑100 (MQ=255)
                                                         ggCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGaa                                       >  2:2780018/1‑100 (MQ=255)
                                                          gCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAg                                      >  2:85790/1‑100 (MQ=255)
                                                            gATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTCAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAggc                                    <  2:5414550/100‑1 (MQ=255)
                                                             aTGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAggcg                                   >  2:2947013/1‑100 (MQ=255)
                                                             aTGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAggcg                                   <  2:4651731/100‑1 (MQ=255)
                                                              tGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAggcgg                                  <  2:418794/100‑1 (MQ=255)
                                                                cTGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCt                                <  2:60020/100‑1 (MQ=255)
                                                                 tGGAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTa                               >  2:559642/1‑100 (MQ=255)
                                                                  ggAAGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTAc                              <  1:1566598/100‑1 (MQ=255)
                                                                    aaGAGTATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACAt                            <  1:3321478/100‑1 (MQ=255)
                                                                         tATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCg                       <  1:4275617/100‑1 (MQ=255)
                                                                         tATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCg                       >  2:3809671/1‑100 (MQ=255)
                                                                         tATCGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCg                       <  1:315413/100‑1 (MQ=255)
                                                                            cGCCATATCCCAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGc                    <  1:2838995/100‑1 (MQ=255)
                                                                                    cccAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTg            >  2:180000/1‑100 (MQ=255)
                                                                                    cccAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTg            >  1:1747954/1‑100 (MQ=255)
                                                                                      cAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGAt          >  2:3759561/1‑100 (MQ=255)
                                                                                      cAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGAt          >  1:2138521/1‑100 (MQ=255)
                                                                                      cAATGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGAt          <  1:1847400/100‑1 (MQ=255)
                                                                                       aaTGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGAtt         <  1:2675874/100‑1 (MQ=255)
                                                                                        aTGGTGGTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTg        <  1:5357585/100‑1 (MQ=255)
                                                                                         tggtggTCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGa       <  1:566295/100‑1 (MQ=255)
                                                                                           gtggtCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAAc     >  2:1465427/1‑100 (MQ=255)
                                                                                           gtggtCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTGCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAAc     <  2:2322255/100‑1 (MQ=255)
                                                                                             ggtCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAAcgc   <  1:3844892/100‑1 (MQ=255)
                                                                                             ggtCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAAcgc   >  1:24005/1‑100 (MQ=255)
                                                                                             ggtCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAAcgc   >  1:2714145/1‑100 (MQ=255)
                                                                                              gtCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAAcgcg  >  2:3298573/1‑100 (MQ=255)
                                                                                              gtCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAAcgcg  >  2:1419523/1‑100 (MQ=255)
                                                                                              gtCCTGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAAcgag  >  2:157897/1‑98 (MQ=255)
                                                                                                 |                                                                                                
GCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCG  >  NC_000913/1738179‑1738372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: