Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,822,043 T→G I72L (ATC→CTC)  osmE ← osmotically‑inducible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,822,0430TG99.5% 657.5 / NA 188I72L (ATC→CTC) osmEosmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (98/89);  total (99/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 196 total aligned reads displayed.

TATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTT  >  NC_000913/1821946‑1822139
                                                                                                 |                                                                                                
 aTTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTa                                                                                               >  2:1827515/1‑100 (MQ=255)
 aTTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTa                                                                                               >  2:2657589/1‑100 (MQ=255)
  ttCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAg                                                                                              <  2:2508138/100‑1 (MQ=255)
   tCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAgg                                                                                             <  2:2269549/100‑1 (MQ=255)
    cAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGaggtaggt                                                                                            >  1:1941328/1‑100 (MQ=255)
    cAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGaggtaggt                                                                                            <  1:4270169/100‑1 (MQ=255)
    cAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGaggtaggt                                                                                            >  2:48798/1‑100 (MQ=255)
     aGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAATTAGGTTTCTGCTTTACCATCACGTTGACCCAGAAGGTAGGTc                                                                                           >  2:106751/1‑100 (MQ=255)
      gCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCt                                                                                          >  2:3213260/1‑100 (MQ=255)
      gCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCt                                                                                          <  1:2963204/100‑1 (MQ=255)
         caGGTCTGATAACCGGAGTTGATGACATGTGCGGTATCATCTAACGCGACCAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGTTTTGGc                                                                                       <  2:5177829/100‑1 (MQ=255)
         caGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACACAGGAGGTAGGGCTgga                                                                                       >  1:186554/1‑99 (MQ=255)
            gTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAg                                                                                    <  1:3037396/100‑1 (MQ=255)
            gTCTGATAACCGGAGTGGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAg                                                                                    >  2:2157634/1‑100 (MQ=255)
              cTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTCGGTCTGGCAAGTa                                                                                  >  2:3866747/1‑100 (MQ=255)
              cTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTa                                                                                  <  1:2286167/100‑1 (MQ=255)
              cTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTa                                                                                  <  1:797183/100‑1 (MQ=255)
               tGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTAc                                                                                 >  2:3779747/1‑100 (MQ=255)
                gATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTAcc                                                                                <  2:239606/100‑1 (MQ=255)
                gATAACCGGAGTTGATGACATGTCCGGGATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGAACCAGGAGGTAGGTCTGGAAAGTAcc                                                                                >  2:2497741/1‑100 (MQ=255)
                 aTAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGAACCAGGAGGTAGGTCTGGCAAGTACcg                                                                               <  1:826460/100‑1 (MQ=255)
                  tAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACcgn                                                                              <  1:571965/100‑2 (MQ=255)
                  tAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACcgc                                                                              <  2:2698509/100‑1 (MQ=255)
                  tAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACcgc                                                                              <  1:1982543/100‑1 (MQ=255)
                     ccGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAg                                                                           >  1:2160872/1‑100 (MQ=255)
                     ccGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAg                                                                           >  2:805963/1‑100 (MQ=255)
                      cGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGc                                                                          <  1:947075/100‑1 (MQ=255)
                         aGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATg                                                                       <  1:134520/100‑1 (MQ=255)
                         aGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGCTTCCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATg                                                                       >  2:656866/1‑100 (MQ=255)
                          gTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATgg                                                                      <  1:4927187/100‑1 (MQ=255)
                           ttGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGa                                                                     <  1:2375463/100‑1 (MQ=255)
                           ttGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGa                                                                     >  2:1472549/1‑100 (MQ=255)
                             gatgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATc                                                                   <  2:3200056/100‑1 (MQ=255)
                             gatgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATc                                                                   <  1:3773986/100‑1 (MQ=255)
                             gatgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATc                                                                   <  1:1765649/100‑1 (MQ=255)
                             gatgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATc                                                                   >  2:5433546/1‑100 (MQ=255)
                              atgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCa                                                                  <  2:777127/100‑1 (MQ=255)
                                 aCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGc                                                               <  1:3883718/100‑1 (MQ=255)
                                 aCATGTCCGGTATCATATAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGc                                                               >  2:5327801/1‑100 (MQ=255)
                                  cATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCt                                                              >  1:2424524/1‑100 (MQ=255)
                                  cATGTCCGGTATCATCTAACGCGACAAAGGAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCt                                                              >  2:1318021/1‑100 (MQ=255)
                                    tGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCa                                                            <  1:1008207/100‑1 (MQ=255)
                                      tCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGATCCATGCTcgcg                                                          <  1:5119974/100‑4 (MQ=255)
                                         ggTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCa                                                       <  2:236152/100‑1 (MQ=255)
                                         ggTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCa                                                       <  1:306990/100‑1 (MQ=255)
                                          gTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAg                                                      >  2:1383183/1‑100 (MQ=255)
                                            atcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGAc                                                    <  1:3142847/100‑1 (MQ=255)
                                            atcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGAc                                                    >  1:1476654/1‑100 (MQ=255)
                                            atcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGAc                                                    >  1:2931193/1‑100 (MQ=255)
                                            atcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGAc                                                    >  2:3365414/1‑100 (MQ=255)
                                            atcatcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGAc                                                    >  1:4493445/1‑100 (MQ=255)
                                            atcatcTAACGCGACAAAGGAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGAc                                                    <  1:3548012/100‑1 (MQ=255)
                                              catcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGa                                                  >  1:1605933/1‑100 (MQ=255)
                                               atcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGaa                                                 >  1:3611032/1‑100 (MQ=255)
                                               atcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGaa                                                 >  2:3916526/1‑100 (MQ=255)
                                                 cTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAgg                                               <  1:3585492/100‑1 (MQ=255)
                                                  tAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGt                                              >  2:4899863/1‑100 (MQ=255)
                                                   aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                             <  2:554984/100‑1 (MQ=255)
                                                   aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                             >  1:2598954/1‑100 (MQ=255)
                                                   aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                             <  1:2485510/100‑1 (MQ=255)
                                                   aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                             <  1:2214261/100‑1 (MQ=255)
                                                   aaCGCGACAAAGTAGGTTTCTACTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                             >  2:4516226/1‑100 (MQ=255)
                                                     cgcgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTa                                           >  1:1171685/1‑100 (MQ=255)
                                                      gcgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAc                                          >  2:3140304/1‑100 (MQ=255)
                                                       cgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAcc                                         <  1:2686568/100‑1 (MQ=255)
                                                       cgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGACTGGCAAGTACGGCGAGCATGGATCATGCTCAATTCAGACGAAGGTTTAcg                                         <  2:2298150/100‑2 (MQ=255)
                                                        gACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAccc                                        <  2:1570220/100‑1 (MQ=255)
                                                         aCAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCg                                       <  2:4089886/100‑1 (MQ=255)
                                                          cAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGc                                      >  2:2783069/1‑100 (MQ=255)
                                                             aGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAt                                   >  1:3507816/1‑100 (MQ=255)
                                                               tAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAttt                                 >  2:330009/1‑100 (MQ=255)
                                                                aGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtg                                <  1:1202335/100‑1 (MQ=255)
                                                                 ggTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgt                               >  2:362038/1‑100 (MQ=255)
                                                                 ggTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgt                               <  1:779526/100‑1 (MQ=255)
                                                                  gTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgtg                              <  2:3801800/100‑1 (MQ=255)
                                                                   tttCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGc                             <  1:864618/100‑1 (MQ=255)
                                                                    ttCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCa                            >  2:1538779/1‑100 (MQ=255)
                                                                    ttCTGCCTTACCATCACGTTGACCCAGGAGGTATGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGCTGACCCGCAATTTGTGCa                            <  2:2818893/100‑1 (MQ=255)
                                                                       tGCTTTACCATCATGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAcc                         <  2:4753113/100‑1 (MQ=255)
                                                                          tttACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCCCGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTgc                      >  2:3449739/1‑100 (MQ=255)
                                                                             aCCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGcc                   <  2:597502/100‑1 (MQ=255)
                                                                             aCCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGcc                   >  1:1644753/1‑100 (MQ=255)
                                                                             aCCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGcc                   >  2:4621530/1‑100 (MQ=255)
                                                                              ccATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGccc                  <  1:2015152/100‑1 (MQ=255)
                                                                               cATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCg                 <  2:4707867/100‑1 (MQ=255)
                                                                                aTCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGACGGTTTACCCGCAATTTGTGAAACCTGCGCCCgg                >  1:5041696/1‑100 (MQ=255)
                                                                                aTCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCgg                >  2:3182340/1‑100 (MQ=255)
                                                                                   aCGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTc             >  2:1535171/1‑100 (MQ=255)
                                                                                    cGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCa            >  2:1680537/1‑100 (MQ=255)
                                                                                    cGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCa            >  2:365944/1‑100 (MQ=255)
                                                                                     gTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGCGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCAt           <  1:3934076/100‑1 (MQ=255)
                                                                                     gTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCAt           <  1:6357/100‑1 (MQ=255)
                                                                                      ttGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATg          <  2:4851550/100‑1 (MQ=255)
                                                                                       tGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGc         <  2:3436896/100‑1 (MQ=255)
                                                                                        gACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGcc        >  2:154592/1‑100 (MQ=255)
                                                                                           ccAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCttt     <  1:3754204/100‑1 (MQ=255)
                                                                                           ccAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCttt     >  2:4293555/1‑100 (MQ=255)
                                                                                            cAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCtttt    >  1:4636518/1‑100 (MQ=255)
                                                                                            cAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCtttt    >  2:1988593/1‑100 (MQ=255)
                                                                                             aggaggTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCttttt   <  2:194977/100‑1 (MQ=255)
                                                                                                 |                                                                                                
TATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTTT  >  NC_000913/1821946‑1822139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: