Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,876,0150AG32.1% 91.5 / 12.4 56I115V (ATT→GTT) yeaNputative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (13/25);  new base G (18/0);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.13e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAA  >  NC_000913/1875788‑1876248
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             
cGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGCATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGGAttttgg                                                                                                                                                                                                                                    >  1:875231/1‑235 (MQ=255)
 gATTCGTTCCGCTTACTCGCTGACGACCGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCTCCTTTGGCTGCCCCGATAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCa                                                                                                                                                                                                                                   <  2:422486/239‑1 (MQ=255)
   ttCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTTCATTAGGCAAt                                                                                                                                                                                                                                 >  1:515157/1‑239 (MQ=255)
            cTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCAGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGCTTTGGTATGGAACGTATCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTTCATTATGGCATGGCTTACt                                                                                                                                                                                                                         >  1:936732/1‑238 (MQ=255)
             ttACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAGTGTCTTACTGc                                                                                                                                                                                                                       <  1:988645/239‑1 (MQ=255)
                    cTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGCATTAAGCAATGTCTTTCTGCCAGGatt                                                                                                                                                                                                                >  2:988645/1‑239 (MQ=255)
                         gacAGCGCAAACCGGCTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCGCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTc                                                                                                                                                                                                                              <  1:1031947/220‑1 (MQ=255)
                                        cTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTc                                                                                                                                                                                           >  1:267860/1‑239 (MQ=255)
                                                   cccTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCg                                                                                                                                                                                  <  1:495383/237‑1 (MQ=255)
                                                             attGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTTCCTTAGGCAAGGTGCTTCTGCCAGAGTTAATTAAATGCGTTTTCCCCCCTTTCCTCGCCAGagcttcc                                                                                                                                                                       >  2:495383/1‑232 (MQ=255)
                                                              ttGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCCGACTTACCg                                                                                                                                                                      >  1:119873/1‑238 (MQ=255)
                                                                ggCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGATTGCATTAAGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTTGCCAGACTTACCGgc                                                                                                                                                                    >  1:316116/1‑238 (MQ=255)
                                                                   cTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCa                                                                                                                                                                 <  1:501533/238‑1 (MQ=255)
                                                                      tGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGGATTAAGCCATGTCTTTCTGCCAGGGTTAATTAAACGCCGATTTCCTCCTTTCCTCGCCCGACCTAACGGCGCatat                                                                                                                                                              >  2:1033282/1‑238 (MQ=255)
                                                                      tGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGCATTAGGCAATGTCTTACTGCCAGGATTTATTAAACGCGGTTTCCCTCCTTCCTGTGCCACACTTAACGGCGCATAtt                                                                                                                                                             >  2:53296/1‑239 (MQ=255)
                                                                      tGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGa                                                                                                                                                                                                                  >  1:640185/1‑187 (MQ=255)
                                                                      tGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGa                                                                                                                                                                                                                  <  2:640185/187‑1 (MQ=255)
                                                                      tGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTaaa                                                                                                                                                                                                         <  2:936995/196‑1 (MQ=255)
                                                                      tGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTaaa                                                                                                                                                                                                         >  1:936995/1‑196 (MQ=255)
                                                                                      ttGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGc                                                                                                                                             <  1:608471/239‑1 (MQ=255)
                                                                                      ttGGCGGCCCGGGTAGCGCGTCGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTGCTTTTGATCTTTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTCCTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGc                                                                                                                                             <  2:96232/239‑1 (MQ=255)
                                                                                              cccGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAg                                                                                                                                        <  2:21904/236‑1 (MQ=255)
                                                                                                 ggTTGCCCGACCTTTTGGTATGGAACGTAGCCTTTTTGGCGCGTTACTTTTGATCTTTGCTGGTAGCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCTGTACAGCGGTCATTGGCGGTGTGATTGCATTAGGCTATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGCCGCCAGACTTACCGTCGCATATTCCCTGACAATGGGAGCTGCAGCGGCAc                                                                                                                                  <  2:472263/239‑1 (MQ=255)
                                                                                                       gcgACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGa                                                                                                                             <  1:571424/238‑1 (MQ=255)
                                                                                                        cgACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGCATTAGGCAATGTCTTTCTGCCAGGGTTAATTAAACGCGATTTCCCTCATTTCGTCGCCCGACTTACCCGCGCATATTCCCTGACAATTGGAGCTGCCGCGGCCCTggggat                                                                                                                           >  2:301309/1‑236 (MQ=255)
                                                                                                        cgACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAAAGCGATTTCCCTCATTTCGTCCCCAGACTTACCCGCGCATATTCCCTGTCAATTGGGGCTGCCGGGGCACTtggggc                                                                                                                           >  2:571424/1‑236 (MQ=255)
                                                                                                          aCGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCCTTAAGCAATGTCTTACTGCCCGGTTTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCCTACCATGGGAGCTGAAGCGGCACCTGGATCg                                                                                                                          >  1:151341/1‑238 (MQ=255)
                                                                                                                        aaCGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTGCCGGCGCATATTCCCTGACAATGGGAGCTGCAg                                                                                                                                        <  1:354872/210‑1 (MQ=255)
                                                                                                                                  gTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGCATTAAGCCATGTCTTACTGCCCGGATTAATTAAACGCGACTTCCCTCATTCCGTCGCCCGCCTTACCGGCGCCTATTTCCTGACAATGGGGGCTGCAGCGGCACTggg                                                                                                                              >  2:354872/1‑210 (MQ=255)
                                                                                                                                         gcgTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGCGGGGTTGGATTAGGCTATGTCTTTCTTCCAGGAATAATTAAACACGATTTCCCCCTCTTTCTCGCCCGACCTTCCAGCGCCAATTTCCTTAAAATTGGAGCTGCATCCGCCCCTGGATTGGCTTTTGGCCTGCCGCTGGCCTTTGacc                                                                                              >  2:322819/1‑233 (MQ=255)
                                                                                                                                            ttACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGGCGTGCCGCTGGCTTTGAACGGGTTtgg                                                                                      >  1:812023/1‑239 (MQ=255)
                                                                                                                                                       cTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGgcg                                                                          <  1:301309/239‑1 (MQ=255)
                                                                                                                                                         gtgCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGcgcg                                                                        <  1:471196/239‑1 (MQ=255)
                                                                                                                                                          tgCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGgcgc                                                                         <  1:331048/237‑1 (MQ=255)
                                                                                                                                                          tgCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGcgcg                                                                        <  1:774694/238‑1 (MQ=255)
                                                                                                                                                             tGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGc                                                                    <  1:398233/239‑1 (MQ=255)
                                                                                                                                                               gTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTTGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTTCCTCATTCCGTCGCCAGACTTACCGGCGCata                                                                                                                                                               >  1:28862/1‑148 (MQ=255)
                                                                                                                                                               gTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCata                                                                                                                                                               <  2:28862/148‑1 (MQ=255)
                                                                                                                                                                    gCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTTCATTAGGGAATGTCTTTCTGCCAGGGTTAATTAAACGGGCTTTCCCTCATTCCGTCGCCAGACTTACCGGCGCAATTTCCCTGACAATGGGGGCCTCGGCGGCGCCCGGCTCGGCCATGGGCGTGCCGCCGGGCTTTTACGGTTTTGGCTGGCTACGCGCGGTGCCCATGc                                                              >  2:331048/1‑238 (MQ=255)
                                                                                                                                                                    gCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGCATTTGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCAt                                                                >  1:969764/1‑236 (MQ=255)
                                                                                                                                                                    gCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGCATTAGGCAATGTCTTTCTGCCAGGATTAATTAAACGCGATTTTCCTCATTCCGTCGCCCGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCCCTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGGACGGGTTTGGCTTGCAAGGCGCGGTGCTCAt                                                                >  2:949728/1‑236 (MQ=255)
                                                                                                                                                                    gCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGTTGCATTAGGCAATGTCTTACTGCCAGGGTTAATTAAAAGCGGTTTTCCTCATTCCGTCGCCAGACTTACCGGCGCACATTCCCTGACAATGGGGGCTGCCGCGGCCCTGGGATCGGCTATGGTCGTGCCCCTGGCTTTGTACGGGTTTGGCTGGGCAGGGGCGTTTGTCAt                                                                >  2:398233/1‑236 (MQ=255)
                                                                                                                                                                    gCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAAGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCCCATTCCGTCGCCCCACTTACCGGCGCATATTTCCCGACAATGGGAGCTGCAGCGGCCCTGGGATCGGCTCTGGTCGTGCCCCTCGCCTTGAACGGGTTTGGTTGGCCAGGGCCGTTTCTCATGc                                                              >  2:774694/1‑238 (MQ=255)
                                                                                                                                                                          cGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGGGCTGCAGCGGCACTGTGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGAtgtg                                                       <  1:1033282/239‑1 (MQ=255)
                                                                                                                                                                                       cGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGGCTGCATTAGGCAATGTCTTACTGCCCGGATTAATTAAACGCGATTTCCCTCCTTCCGTCGCCAGACTTACCGGCGCTTATTCCCTTACAATGGGAGCTGCAGCGGGCCTGGGCTCGGCCATGGTCGTGCCCCCTGCTTTGTAAGGTTTTTGTTTGCCAGGCCCGCTGTCCCTGCTTTTGTGGTTTTCTctgctt                                          >  2:3602/1‑238 (MQ=255)
                                                                                                                                                                                             acttaTTATTTGGCGGTACAGCGGTCATTGGCGGGGGGGTTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAAAGCGATTTCCCCCATTCCGTCGCCAGACTTACCCGCGCATATTCCCTGACTCTGGGGGCTGCGGCGGCACTGGGATCGGCCATGGTCGTTCCGCTGGCCTTGTACGGTTTTGGCTGGCAAGGCCCGTGTCTCATTCTGCTTTGTTTTCTTCtggtggg                                        >  2:790218/1‑234 (MQ=255)
                                                                                                                                                                                                 attattTGGCGGTACAGCGGTCATTGGCGGGGGGATTGCATTTGGCAATTTCTTTCTGCCCCGGTTTATTTAACGCGCTTTTCCTCATTTCGTCGCCCGACTTACCGGCGCATATTTCCTTACAATTGGAGCTGCCGCGGCACTGGGATCGGCTATGGTCGTGCCGCCGGCTTTTAACGGGTTTTGCTGGCCATGCGCGTTGCTCATGCTGTTGTGTTTTTCTTTTCTTGCTTcttttt                                >  2:701815/1‑239 (MQ=255)
                                                                                                                                                                                                   tattTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCCTTAGGCAATTTCTTACTTCCAGGGTTTATTAAACGCGATTTTCCTCCTTCCGTCTCCCGAATTTCCGGCGCATATTCCCTGTCCATTGGGGCTGCCGCGGCCCCGGGGTCGGCTCAGGTCCGGCCCCCTGGTTTTAACGGTTTTTGCTGGCACAGGCCGGTGGTCCTTCTGTTGTGTGTTCTCCTGCTGGGTCtttttttt                              >  2:503516/1‑238 (MQ=255)
                                                                                                                                                                                                    attTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTAt                             <  2:267860/239‑1 (MQ=255)
                                                                                                                                                                                                        ggCGGTACAGCGGTCATTGGCGGTGGGATTGCCTTAGGCAATGTCTTTCTGCCAGGATTAATTAAAcg                                                                                                                                                                                                       >  2:168301/1‑68 (MQ=255)
                                                                                                                                                                                                        ggCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAAcg                                                                                                                                                                                                       <  1:168301/68‑1 (MQ=255)
                                                                                                                                                                                                           ggTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGGACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGc                       <  1:38506/238‑1 (MQ=255)
                                                                                                                                                                                                             tACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCttttttt                               <  1:523001/228‑1 (MQ=255)
                                                                                                                                                                                                                      cATTGGCGGTGGGATTGCCTTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGGTTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGGGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAGCGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGaa          <  2:936732/239‑1 (MQ=255)
                                                                                                                                                                                                                       aTTGGCGGTGGGGTTGCATTAGGCAATGTCTTACTGCCAGGGTTAATTAAAAGCGATTTCCCTCATTCCGTCGCCCGACTTACCGGCGCATATTTCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCCATGGGCGTGCCGCCGGCTTTTGACGGGTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTTTTCTGGGTCTTTTTTTTTTGCTGccc                     >  2:523001/1‑227 (MQ=255)
                                                                                                                                                                                                                               tGGGATTGCATTAGGCAATGTCTTTCTGCCAGGAATAATTAAACGCGATTTCCCTCATTCCGTCGCCCGACCTACCGGCGCATATTCCCTGACAATGGGGTCTTCAGCCGCACTGGGATCGGCCCTGGTCGTGGCGCCGGCTTTGAACGGTTTTTGCTGGCCAGGCGCGGGTCTCATTCCGATGTGTTTTCCTCTTCTTGGTCCTTTTTTTTGGCTGCCACACTGGGGCAAGTcaaaac  >  2:823105/1‑236 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             
CGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAA  >  NC_000913/1875788‑1876248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: