Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,332,8230AG34.2% 54.3 / 17.9 38pseudogene (1580/4605 nt)yfaSpseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/24);  new base G (13/0);  total (14/24)
Fisher's exact test for biased strand distribution p-value = 2.59e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTTCGAGACGCAACCATTCATACGTAACAGGAACCTGTTTTGAAGATTCCAGCGCGGCATAACGGAACACAACCGACTCGCCGCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCA  >  NC_000913/2332593‑2333053
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
cTTCGAGACGCACCCATTCATCCGTAACAGGAACCTTTTTTGAAGATTCCAGCGCGGCATAACGGAACACAACCGACTCGCCGCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTaa                                                                                                                                                                                                                                            <  2:472848/238‑1 (MQ=255)
 ttCGAGACGCAACCATTCATACGTAACAGGAACCTGTTTTGAAGATTCCAGCGCGGCATAACGGAACACAACCGACTCGCCGCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACg                                                                                                                                                                                                                                          <  1:946403/239‑1 (MQ=255)
                               aaCCTGTTTTGAAGATTCCAGCGCGGCATAACGGAACACAACCGACTCGCCGCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCATCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGac                                                                                                                                                                                                                 <  1:469590/232‑1 (MQ=255)
                                aCCTGTTTTGAAGATTCCAGCGCGGCATAACGGAACACAACCGACTCGCCGCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGGTTATCGGCGGCGGGGGGGAGTTAAGGCCCATGAACGCTGGGGTCGGTCAcccgttc                                                                                                                                                                                                        >  2:655374/1‑234 (MQ=255)
                                                     gcgGCATAACGGAACACAACCGACTCGCCGCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGaca                                                                                                                                                                                   <  1:683439/235‑1 (MQ=255)
                                                     gcgGAAAAACGGAACACAACCGACGCGCCGCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGaca                                                                                                                                                                                   <  2:4126/235‑1 (MQ=255)
                                                             aCGGAACACAACCGACTCGCCGCTATTACTGTATTGGGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTc                                                                                                                                                                      <  1:194888/239‑1 (MQ=255)
                                                                            cTCGCCGCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCaaa                                                                                                                                                       <  1:388402/238‑1 (MQ=255)
                                                                                  gcTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTAc                                                                                                                                               <  1:253934/239‑1 (MQ=255)
                                                                                      ttACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCAt                                                                                                                                                                <  1:259790/220‑1 (MQ=255)
                                                                                        aCTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCa                                                                                                                                         <  1:45460/239‑1 (MQ=255)
                                                                                          tGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCAtt                                                                                                                                       <  1:547743/239‑1 (MQ=255)
                                                                                              ttGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGGGTTTTACCCCCCCAAACCCCCGGGTGTGGGACCCCGTTTCCCTGCCTCCCCCCGCCACGGGGAAACGCCGGCCAAAACCAAAACCGTTCCGACCATTGTTa                                                                                                                                   >  2:360301/1‑239 (MQ=255)
                                                                                                 tgCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGGGTTTTAACCCCCATGATCGCCGGGGGCGGAGACCGGTTCGCTGCCTTTCCTCGGCCACGGGGAACGGTCCGCCAAAACCCAAATGTTTACCACCCATGATaaat                                                                                                                                >  2:600963/1‑237 (MQ=255)
                                                                                                        gTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGGATTTTACGCCACATGACCCCCGGCGTCGGACACCCTTTTGCTGCCTTCCAGCGCCACGGGGAAACGTCCGGCCTAACGCCAATCGTTACCCACCATTGATACTTGCTGAg                                                                                                                          >  1:337615/1‑238 (MQ=255)
                                                                                                           cTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAgcgcg                                                                                                                     <  1:360301/239‑1 (MQ=255)
                                                                                                                  aGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAAc                                                                                                                <  1:732067/237‑1 (MQ=255)
                                                                                                                   gTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGGGATTTACGCCCCCATACCGCCGGCGGCGGGGCACCGTTTGCTGCCCTCCCGCGCACACGGGGAAACGCCCGGCTAACGCCAAACTCTTCCCGCCCATTTTTAATTGTGTGCGCCGCAAACtt                                                                                                              >  2:298519/1‑239 (MQ=255)
                                                                                                                      aTGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTTACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTTCTGAGCGCGCAAACTTa                                                                                                             >  1:704941/1‑236 (MQ=255)
                                                                                                                          gcCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCAc                                                                                                      <  1:600963/239‑1 (MQ=255)
                                                                                                                            cAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGGATTTTACGCCACATGACCGCTGGCGGCGGGCACCCGTTTCCCGCCCTCCAGCCACACGGGGGAACGTCCCGCGTAAAGCAAAACGTTACCCACCCTTTATAATTGCTGCGCGCGCGAACCTAAATCCAca                                                                                                     >  2:732067/1‑237 (MQ=255)
                                                                                                                                      tCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGGATTTAACGCCACATGACCGCCGGCGTCGGACACCGTTTCGCTGCCTTCCCGCGACACGGGGAAACGGCCGGCATAACGCAAATCGTTACCGACCCTTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCAttttt                                                                                          >  1:759197/1‑239 (MQ=255)
                                                                                                                                           gAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGGGGGGGGGGTTTAAGCCCCAAGAACGCCGGGGGTGGGCACCCGTTTGCGGGCCTTCCACGGCACGGGGGAAACGCCGGCCCAAACGCAAACGCTTCCACCCCATTGTAAATGGCGGAGCGCCAAAACTAAACCGCACGCGGGCAtttttt                                                                                         >  2:746197/1‑236 (MQ=255)
                                                                                                                                              gATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTAc                                                                                                                                               <  1:639357/179‑1 (MQ=255)
                                                                                                                                                 ctctTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCTTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTACTGAGCGCGCa                                                                                                                   <  1:979642/203‑1 (MQ=255)
                                                                                                                                                  tctTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGGATTTTACGCCCCATGACCGCTGGCGTCGGGCACCGGTTCGCTGCCTTCCCGCGGCCAGGGGGAAAGCCCGGCCTAACGCAAAACGTTAACCACCCTTGATAATTGCTGAGCGCGCACACTTAAATGAACGCGGGGATTTTTTTCCCGCTCGc                                                                               >  2:389455/1‑238 (MQ=255)
                                                                                                                                                        gtgggggtgACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGGTTATCGGCGGCGGGGGGAGTTAACATCAAATGAACCCGGGGGGCGGACACCGGTTCGTCTGCCTCTATCGACACGGGGGAAAGTTCGGCACAACCTCAAACGTTACCGACCACTgt                                                                                                                                     >  2:639357/1‑178 (MQ=255)
                                                                                                                                                           gtggtgACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGGGATTTACGCCCCCTTACCGCTGGGCTCGGGCACCGCTTTGCTGCCTTTCCGCGACCCGGGGGAACGTCCGGCATAACGCAAATCGTTTCCCACCATTTATAAATTCTCAGTGCGCAAACtttaatc                                                                                                         >  2:979642/1‑198 (MQ=255)
                                                                                                                                                            tggtgACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCCCGGTTTATCGGCGGCGGGGGGATTTAAAGCCCCAATACCGCTGGGGGCCGAAACCCGTTCGCTGCCTTCCCGCGACCCGGGGAAACGGCCCGCATAACGCCAAACGTTACCCAGCATTGATAATTTCTGAGCGCGCAAAATTAACTGCCCGCGGGCCATTTTTTCCGGCTCGCCATCCcgggtg                                                                    >  2:803371/1‑236 (MQ=255)
                                                                                                                                                             ggtgACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTag                                                                    <  1:82505/238‑1 (MQ=255)
                                                                                                                                                                      aTACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGGTTATCGGCGGCGGGGGGGATTTACGCCCCCTTACCGCCGGGCGCTGGCCCCCCTTTGCTGCCCTTCCCCGAACCGGGGGAACCGTCCGCCTAACGCAAAACGTTTACCCTCCATTATAAATTGCTGGCGCCCCAACCTAACAGCACCCGGGGATTTTTTTCCTGCGCGCCCTCCCGGGTGCgcagtttca                                                          >  2:637976/1‑239 (MQ=255)
                                                                                                                                                                       tACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAgcagttgca                                                          <  1:238748/238‑1 (MQ=255)
                                                                                                                                                                                 cgTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCt                                                <  2:704941/238‑1 (MQ=255)
                                                                                                                                                                                                   ttAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCAt                                                                            <  1:777106/192‑1 (MQ=255)
                                                                                                                                                                                                    tAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGtt                                <  1:631537/235‑1 (MQ=255)
                                                                                                                                                                                                                  tCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCg              <  1:746197/239‑1 (MQ=255)
                                                                                                                                                                                                                  tCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCg              <  2:950268/239‑1 (MQ=255)
                                                                                                                                                                                                                               ggcggGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCa  <  1:660587/238‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
CTTCGAGACGCAACCATTCATACGTAACAGGAACCTGTTTTGAAGATTCCAGCGCGGCATAACGGAACACAACCGACTCGCCGCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGGTTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAGATTTAACGCCACATGACCGCTGGCGTCGGACACCGTTTCGCTGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCA  >  NC_000913/2332593‑2333053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: