breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsGlucose_ALE_8_FLASK_380_Isolate_0_pop_S3_L001_R1_001.good.fq233,07446,943,429100.0%201.4 bases239 bases98.7%
errorsGlucose_ALE_8_FLASK_380_Isolate_0_pop_S3_L001_R2_001.good.fq233,07446,917,825100.0%201.3 bases239 bases94.2%
total466,14893,861,254100.0%201.4 bases239 bases96.4%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65220.32.9100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000006313
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500025
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.003

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.95744

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input12:56:50 23 May 201912:56:57 23 May 20197 seconds
Read alignment to reference genome12:56:57 23 May 201912:58:35 23 May 20191 minute 38 seconds
Preprocessing alignments for candidate junction identification12:58:35 23 May 201912:58:41 23 May 20196 seconds
Preliminary analysis of coverage distribution12:58:41 23 May 201912:59:02 23 May 201921 seconds
Identifying junction candidates12:59:02 23 May 201912:59:17 23 May 201915 seconds
Re-alignment to junction candidates12:59:17 23 May 201912:59:43 23 May 201926 seconds
Resolving best read alignments12:59:43 23 May 201912:59:54 23 May 201911 seconds
Creating BAM files12:59:54 23 May 201913:00:13 23 May 201919 seconds
Tabulating error counts13:00:13 23 May 201913:00:20 23 May 20197 seconds
Re-calibrating base error rates13:00:20 23 May 201913:00:21 23 May 20191 second
Examining read alignment evidence13:00:21 23 May 201913:02:55 23 May 20192 minutes 34 seconds
Polymorphism statistics13:02:55 23 May 201913:02:56 23 May 20191 second
Output13:02:56 23 May 201913:03:11 23 May 201915 seconds
Total 6 minutes 21 seconds