Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8520CT24.3% 318.8 / 103.7 181intergenic (+19/+27)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (73/64);  new base T (19/25);  total (92/89)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 183 total aligned reads displayed.

GAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAC  >  NC_000913/3171754‑3171950
                                                                                                  |                                                                                                  
   tCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGt                                                                                                 <  2:2229471/99‑1 (MQ=255)
     gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTa                                                                                               >  1:2239743/1‑99 (MQ=255)
       cAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGCGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaa                                                                                             >  2:137192/1‑99 (MQ=255)
       cAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaa                                                                                             <  1:2694108/99‑1 (MQ=255)
        aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                            >  1:3531848/1‑99 (MQ=255)
        aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                            <  1:1915338/99‑1 (MQ=255)
        aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                            >  1:1825993/1‑99 (MQ=255)
            tGCATGGCATGAATGTTGAAGTTGGGAATGCGGAACAGGGTGGATTTGTGGTGAAGGTAAGCTGGTAACTTTTCTGTCTATGAGAGCCGTTAAAACGAc                                                                                        <  2:2735907/99‑1 (MQ=255)
                tggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACtctc                                                                                    >  1:2384469/1‑99 (MQ=255)
                tggcatgAATGTTGAATGTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCtctc                                                                                    <  1:2836337/99‑1 (MQ=38)
                 ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCa                                                                                   <  2:3203786/99‑1 (MQ=38)
                 ggcatgAATGTTGAATGTGGGAATGCGGAACAAGGTGGATTTGAGGCTAAGTTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCa                                                                                   <  2:2638636/99‑1 (MQ=255)
                 ggcatgAATGTGGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCa                                                                                   <  2:1946253/99‑1 (MQ=38)
                  gcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCAt                                                                                  <  1:659132/99‑1 (MQ=255)
                   catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACTGCTCTCATa                                                                                 <  2:3540077/99‑1 (MQ=38)
                   catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATa                                                                                 <  1:173524/99‑1 (MQ=38)
                   catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATa                                                                                 >  1:551587/1‑99 (MQ=255)
                    atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTGTGAGAGTCGTTTTAACGGCTCTCATAg                                                                                <  1:3486729/99‑1 (MQ=38)
                    atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAg                                                                                <  1:548353/99‑1 (MQ=38)
                    atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAg                                                                                >  1:3038407/1‑99 (MQ=255)
                    atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAg                                                                                >  2:2418205/1‑99 (MQ=255)
                    atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCGCTGTCTATGAGAGCCGTTAAAACGACTCTCATAg                                                                                >  1:588999/1‑99 (MQ=255)
                     tgAATGTTGAATTTGGGAATGCGGAACGAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGa                                                                               <  1:1027484/99‑1 (MQ=255)
                     tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGa                                                                               <  1:986584/99‑1 (MQ=37)
                     tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGa                                                                               <  1:3348291/99‑1 (MQ=38)
                       aaTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTGAACGGCTCTCATAGaca                                                                             <  1:3300230/99‑3 (MQ=38)
                        aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGacag                                                                            <  1:1789260/99‑4 (MQ=37)
                         tGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGacaga                                                                           <  1:2884760/99‑5 (MQ=25)
                         tGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGacaga                                                                           <  2:2122879/99‑5 (MQ=25)
                          gTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTTTCATAGATTTTa                                                                          <  2:3422340/99‑1 (MQ=255)
                           ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                         <  2:880734/99‑1 (MQ=255)
                           ttGAATTAGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                         >  2:3691060/1‑99 (MQ=255)
                            tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                        <  1:819439/99‑1 (MQ=255)
                              aaTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                      <  2:165628/99‑1 (MQ=255)
                                tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                    <  2:3222459/99‑1 (MQ=255)
                                 ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                   >  1:2188904/1‑99 (MQ=255)
                                 ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                   >  2:2312302/1‑99 (MQ=255)
                                    ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTACCTTCTGTGTCTATTAGAGCCGTTAAAAAGACGCTCATAGATTTTACTAATAGcca                                                                >  2:973199/1‑97 (MQ=255)
                                     gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaa                                                               >  2:908616/1‑99 (MQ=255)
                                       aTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAat                                                             <  1:2560769/99‑1 (MQ=255)
                                        tGCGGAACAAGGGGGATTTGCGGCGACGGTAAGCTCGTAATTTATATGTCTATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCCAGata                                                            <  2:2960402/99‑1 (MQ=255)
                                           ggAACAAGGTGGATGTGGGGCGAAGGTAAGCTGGTAAGTTCTCTGTCTATGAGAGCGGCTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaa                                                         >  2:1900192/1‑99 (MQ=255)
                                            gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                        >  1:1018146/1‑99 (MQ=255)
                                             aaCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                       <  1:314900/99‑1 (MQ=255)
                                                  ggtggtTGTGAGGCGAAGGTAAGATGGTAAATTGTCGGTCTATGCGAGCCGTTAAAACAACAATCATAGATTTTACTAATAGCTAAATATAAACCGTcc                                                  >  2:739994/1‑99 (MQ=255)
                                                   gTGGATTTGAGGCGGAGGTAAGGTGGTAATGTCTCTGTCTATGAGAGCCGTTAAAATGACTCTCATAGATTTTACTAATAGCAAGATATAAACCGTccc                                                 <  2:737986/99‑1 (MQ=255)
                                                   gTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTccc                                                 <  1:167929/99‑1 (MQ=255)
                                                    tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcccc                                                >  2:1556729/1‑99 (MQ=255)
                                                    tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcccc                                                >  1:2153545/1‑99 (MQ=255)
                                                     ggATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCa                                               >  2:3214287/1‑99 (MQ=255)
                                                     ggATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCa                                               >  1:2346543/1‑99 (MQ=255)
                                                      gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTCATAGCCAAATATAAACCGTCCCCaa                                              >  2:3078266/1‑99 (MQ=255)
                                                       aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaa                                             <  1:2931188/99‑1 (MQ=255)
                                                        attGAGGCGAAGGTAAGCTGATAACAACACTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTATACTAATAACCACATATAAACCGTCCCCaaaa                                            >  2:3626305/2‑99 (MQ=255)
                                                          tGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                          <  2:2018414/99‑1 (MQ=255)
                                                          tGAGGCAAAGGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTTAAAACGACGCTCATAGATTTTACTAATAGCAAAATATAAACCGTCAACaaaaaa                                          <  2:2781905/99‑1 (MQ=255)
                                                              gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                      <  2:1193134/99‑1 (MQ=255)
                                                              gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                      >  2:182816/1‑99 (MQ=255)
                                                                   ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaac                                 >  2:1403189/1‑99 (MQ=255)
                                                                    gTAAGCTGGTAACTTCTCTCTCTATGACAGCCGTTAAAACCACTCTCATAGATTTTACTAATAGCCCAATATAAATCACCCCCAAAAAAGTCaccaccc                                >  2:784959/1‑99 (MQ=255)
                                                                     tAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacca                               >  2:2498552/1‑99 (MQ=255)
                                                                     tAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacca                               >  2:283207/1‑99 (MQ=255)
                                                                      aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                              >  2:3750463/1‑99 (MQ=255)
                                                                         cTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaaa                           >  1:3101828/1‑99 (MQ=255)
                                                                         cTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaaa                           >  1:1257177/1‑99 (MQ=255)
                                                                          tGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                          >  1:1837665/1‑99 (MQ=255)
                                                                             tAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCCCAAACCAg                       <  2:406558/99‑1 (MQ=255)
                                                                              aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                      <  1:1615727/99‑1 (MQ=255)
                                                                               aCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                     <  1:1178053/99‑1 (MQ=255)
                                                                               aCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                     >  1:1541174/1‑99 (MQ=255)
                                                                                cTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACa                    >  1:3240300/1‑99 (MQ=255)
                                                                                 ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCGACCACAAACCAGACaa                   >  1:3253737/1‑99 (MQ=255)
                                                                                 ttCTCTGTCTATGAGACCCGTTAAAACGACTCTCAGAGATTTTCCTAATAGCAAAGTATAAACCGTCCCCAAAAAAGCCACCCACCACAAACAAGACaa                   >  2:1177530/1‑99 (MQ=255)
                                                                                     ctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc               <  1:3826173/99‑1 (MQ=255)
                                                                                     ctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc               >  1:3604002/1‑99 (MQ=255)
                                                                                     ctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc               <  1:904389/99‑1 (MQ=255)
                                                                                      tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAcc              <  1:1955211/99‑1 (MQ=255)
                                                                                      tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAcc              >  1:1366496/1‑99 (MQ=255)
                                                                                      aatCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAcc              >  1:3756438/3‑99 (MQ=37)
                                                                                       gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg             <  2:1091047/99‑1 (MQ=255)
                                                                                       atCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg             >  1:1702899/2‑99 (MQ=37)
                                                                                       atCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg             >  1:1074743/2‑99 (MQ=37)
                                                                                        tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga            <  1:3898238/99‑1 (MQ=255)
                                                                                        tCTATGAGAGCCGTTAAAACGACGCTGACAGATTTTACTAATAGCAAGATATAAACAGACAAAAAAAAAGCCACCAACCACAAACCAGACAAATACcga            <  2:123043/99‑1 (MQ=255)
                                                                                         cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag           >  1:3201210/1‑99 (MQ=38)
                                                                                         cTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag           <  1:1296069/99‑1 (MQ=255)
                                                                                         cTATAAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGCCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcggg           <  2:374369/99‑3 (MQ=255)
                                                                                          tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc          >  1:1719459/1‑99 (MQ=38)
                                                                                          tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc          >  1:3372975/1‑99 (MQ=38)
                                                                                          tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc          >  1:262340/1‑99 (MQ=38)
                                                                                          tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc          >  2:1633625/1‑99 (MQ=38)
                                                                                          tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc          >  2:3273535/1‑99 (MQ=38)
                                                                                            tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAGACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAGAAATACtgagcga        >  1:3568178/1‑99 (MQ=37)
                                                                                            tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga        >  1:2087645/1‑99 (MQ=38)
                                                                                            tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga        >  2:3449388/1‑99 (MQ=38)
                                                                                            tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga        >  1:946313/1‑99 (MQ=38)
                                                                                               gagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa     >  1:860475/1‑99 (MQ=255)
                                                                                                agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGATAAt    <  1:1292360/99‑1 (MQ=255)
                                                                                                agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt    <  1:601440/99‑1 (MQ=255)
                                                                                                agCCGTTAAAACGACGCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt    <  2:3478220/99‑1 (MQ=255)
                                                                                                  |                                                                                                  
GAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAC  >  NC_000913/3171754‑3171950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: