Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8580AT24.7% 318.1 / 124.4 175intergenic (+25/+21)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (71/60);  new base T (19/24);  total (90/85)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 188 total aligned reads displayed.

CCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAC  >  NC_000913/3171760‑3171956
                                                                                                  |                                                                                                  
ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                    <  2:2837297/99‑1 (MQ=255)
 cAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGCGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaa                                                                                                   >  2:137192/1‑99 (MQ=255)
 cAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaa                                                                                                   <  1:2694108/99‑1 (MQ=255)
  aaaTTGCATGGCATGAATGTTGAATTTGGGAATGTGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                                  <  1:3622705/99‑1 (MQ=255)
  aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                                  <  1:1915338/99‑1 (MQ=255)
  aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                                  >  1:1825993/1‑99 (MQ=255)
   aaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAc                                                                                                 <  2:1331197/99‑1 (MQ=255)
        catggtatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCCAAGGTAATCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActc                                                                                            <  1:3757661/99‑1 (MQ=255)
          tggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACtctc                                                                                          >  1:2384469/1‑99 (MQ=255)
           ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCa                                                                                         <  1:3193217/99‑1 (MQ=38)
           ggcatgAATGTTGAATGTGGGAACGCGGAACTATGTTGATTTGAGGCGAAGGTAAGCTGGCAACTTCTCTCTCTATGAGAGTCGTTTTAACGGCTCTCa                                                                                         <  2:3494400/99‑1 (MQ=25)
           ggcatgAATGTGGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCa                                                                                         <  2:1946253/99‑1 (MQ=38)
            gcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCAt                                                                                        <  1:659132/99‑1 (MQ=255)
             catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATa                                                                                       <  2:2818696/99‑1 (MQ=38)
             catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATa                                                                                       >  1:551587/1‑99 (MQ=255)
              atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAg                                                                                      >  1:3038407/1‑99 (MQ=255)
              atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCGCTGTCTATGAGAGCCGTTAAAACGACTCTCATAg                                                                                      >  1:588999/1‑99 (MQ=255)
               tgAATGTTGAATTTGGGAATGCGGAACGAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGa                                                                                     <  1:1027484/99‑1 (MQ=255)
               tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGa                                                                                     <  2:342706/99‑1 (MQ=37)
               tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGa                                                                                     >  2:3106351/1‑99 (MQ=255)
                gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGac                                                                                    <  2:2367367/99‑2 (MQ=37)
                gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGac                                                                                    <  2:265249/99‑2 (MQ=37)
                 aaTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTATCTATGAGAGTCGTTTTAACGGCTCTCATAGaca                                                                                   <  1:3351900/99‑3 (MQ=25)
                  aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGacag                                                                                  <  1:1789260/99‑4 (MQ=37)
                  aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAttt                                                                                  <  2:2427829/99‑1 (MQ=255)
                  aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAttt                                                                                  <  2:484302/99‑1 (MQ=255)
                   tGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGacaga                                                                                 <  2:2122879/99‑5 (MQ=25)
                     ttGAATTAGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                               >  2:3691060/1‑99 (MQ=255)
                      tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                              <  1:819439/99‑1 (MQ=255)
                        aaTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                            <  2:165628/99‑1 (MQ=255)
                        aaTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                            <  1:321672/99‑1 (MQ=255)
                          tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                          <  2:3222459/99‑1 (MQ=255)
                          tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                          <  1:2837869/99‑1 (MQ=255)
                           ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                         >  1:2188904/1‑99 (MQ=255)
                              ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTACCTTCTGTGTCTATTAGAGCCGTTAAAAAGACGCTCATAGATTTTACTAATAGcca                                                                      >  2:973199/1‑97 (MQ=255)
                              ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                      >  2:3816807/1‑99 (MQ=255)
                                 aTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAat                                                                   <  1:2560769/99‑1 (MQ=255)
                                   gCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAatat                                                                 >  2:3206275/1‑99 (MQ=255)
                                     ggAACAAGGTGGATGTGGGGCGAAGGTAAGCTGGTAAGTTCTCTGTCTATGAGAGCGGCTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaa                                                               >  2:1900192/1‑99 (MQ=255)
                                      gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                              <  1:126169/99‑1 (MQ=255)
                                      gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                              <  2:1563150/99‑1 (MQ=255)
                                      gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                              >  1:1018146/1‑99 (MQ=255)
                                       aaCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                             <  2:2969221/99‑1 (MQ=255)
                                           aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTc                                                         >  1:383042/1‑99 (MQ=255)
                                            ggtggtTGTGAGGCGAAGGTAAGATGGTAAATTGTCGGTCTATGCGAGCCGTTAAAACAACAATCATAGATTTTACTAATAGCTAAATATAAACCGTcc                                                        >  2:739994/1‑99 (MQ=255)
                                              tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcccc                                                      >  1:2153545/1‑99 (MQ=255)
                                                 aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaa                                                   <  1:2931188/99‑1 (MQ=255)
                                                  tttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaa                                                  >  2:3379557/1‑99 (MQ=255)
                                                  attGAGGCGAAGGTAAGCTGATAACAACACTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTATACTAATAACCACATATAAACCGTCCCCaaaa                                                  >  2:3626305/2‑99 (MQ=255)
                                                    tGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                <  2:2018414/99‑1 (MQ=255)
                                                    tGAGGCAAAGGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTTAAAACGACGCTCATAGATTTTACTAATAGCAAAATATAAACCGTCAACaaaaaa                                                <  2:2781905/99‑1 (MQ=255)
                                                     gAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                               >  1:95648/1‑99 (MQ=255)
                                                       ggCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcc                                             >  1:3337562/1‑99 (MQ=255)
                                                        gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                            <  2:1193134/99‑1 (MQ=255)
                                                         cGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccac                                           >  1:3436828/1‑99 (MQ=255)
                                                          gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                          >  2:3476707/1‑99 (MQ=255)
                                                            aGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaa                                        >  1:1629391/1‑99 (MQ=255)
                                                             ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaac                                       >  2:1403189/1‑99 (MQ=255)
                                                              gTAAGCTGGTAACTTCTCTCTCTATGACAGCCGTTAAAACCACTCTCATAGATTTTACTAATAGCCCAATATAAATCACCCCCAAAAAAGTCaccaccc                                      >  2:784959/1‑99 (MQ=255)
                                                                aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                    >  2:3750463/1‑99 (MQ=255)
                                                                aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                    >  1:1154409/1‑99 (MQ=255)
                                                                   cTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaaa                                 >  1:3101828/1‑99 (MQ=255)
                                                                    tGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                >  1:1837665/1‑99 (MQ=255)
                                                                     ggTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAcc                               <  1:3527784/99‑1 (MQ=255)
                                                                      gTAACTTCTGTGTCTATGAGAGCCGGTAAAACTACTCTCATAGATTTTACTAATAGCAAAATATAATCCGTCCCCAAAAAAGCCACCAACCACAAACCa                              <  2:2517749/99‑1 (MQ=255)
                                                                        aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                            <  1:1600688/99‑1 (MQ=255)
                                                                         aCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                           >  1:1541174/1‑99 (MQ=255)
                                                                          cTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACa                          >  1:3240300/1‑99 (MQ=255)
                                                                               ctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                     <  1:904389/99‑1 (MQ=255)
                                                                               ctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                     >  1:3604002/1‑99 (MQ=255)
                                                                                tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAcc                    <  1:1955211/99‑1 (MQ=255)
                                                                                tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAcc                    >  1:1366496/1‑99 (MQ=255)
                                                                                 atCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                   >  1:1074743/2‑99 (MQ=37)
                                                                                  tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga                  >  1:1697286/1‑99 (MQ=255)
                                                                                  tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga                  >  2:1159084/1‑99 (MQ=255)
                                                                                  tCTATGAGAGCCGTTAAAACGACGCTGACAGATTTTACTAATAGCAAGATATAAACAGACAAAAAAAAAGCCACCAACCACAAACCAGACAAATACcga                  <  2:123043/99‑1 (MQ=255)
                                                                                   cTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                 <  1:1296069/99‑1 (MQ=255)
                                                                                   cTATAAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGCCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcggg                 <  2:374369/99‑3 (MQ=255)
                                                                                    tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                >  1:1719459/1‑99 (MQ=38)
                                                                                    tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                >  1:2268728/1‑99 (MQ=38)
                                                                                    tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                >  2:3273535/1‑99 (MQ=38)
                                                                                    tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                >  1:262340/1‑99 (MQ=38)
                                                                                    tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                >  2:1869756/1‑99 (MQ=255)
                                                                                    tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                >  1:500372/1‑99 (MQ=255)
                                                                                      tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAGACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAGAAATACtgagcga              >  1:3568178/1‑99 (MQ=37)
                                                                                      tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga              >  1:2087645/1‑99 (MQ=38)
                                                                                      tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga              >  1:946313/1‑99 (MQ=38)
                                                                                       gagaGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCGAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag             >  2:1083701/1‑99 (MQ=38)
                                                                                       gagaGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag             >  2:1815188/1‑99 (MQ=255)
                                                                                        agagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga            <  2:895074/99‑1 (MQ=255)
                                                                                         gagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa           >  1:860475/1‑99 (MQ=255)
                                                                                          agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt          <  1:601440/99‑1 (MQ=255)
                                                                                          agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt          >  1:2764231/1‑99 (MQ=255)
                                                                                            ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGCCAAGTACCGAGCGAGAATAc        >  1:3260222/1‑99 (MQ=255)
                                                                                              gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGGGCGAGAATACat      <  1:3121455/99‑1 (MQ=255)
                                                                                              gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACat      <  1:1191152/99‑1 (MQ=255)
                                                                                              gTTAAAACGACTCGCATAGATTATACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGTCACATACCGTGCGAGAATACat      <  2:924981/99‑1 (MQ=255)
                                                                                               ttAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata     >  2:824546/1‑99 (MQ=255)
                                                                                                 aaaaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACAtata   >  2:1523827/1‑99 (MQ=255)
                                                                                                  aaaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAc  >  2:3339712/1‑99 (MQ=255)
                                                                                                  |                                                                                                  
CCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAC  >  NC_000913/3171760‑3171956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: