BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,472,437	0	T	C	20.8%	295.4 / 76.0	159	N33D (AAT→GAT)	pyrL	pyrBI operon leader peptide
Reads supporting (aligned to +/- strand): ref base T (66/60); new base C (28/5); total (94/65)
Fisher's exact test for biased strand distribution p-value = 6.39e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 163 total aligned reads displayed.

ATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATAC  >  NC_000913/4472339‑4472535

aTCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGAtt                                                                                                    >  1:413815/1‑99 (MQ=255)

  cgcgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGa                                                                                                  >  1:2378222/1‑99 (MQ=255)

   gcgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAg                                                                                                 <  1:64706/99‑1 (MQ=255)

   gcgACTAAGGTCGTTTATGGAAATCATATGTTTCTGATATAGCGCATTAGCCATCTTTTATATCCTTACACCTGTGCAAAAAAAAGCCCCTCGATTgtg                                                                                                 <  2:248166/99‑3 (MQ=255)

     gACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAggg                                                                                               >  1:2101439/1‑99 (MQ=255)

        tAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCt                                                                                            >  2:1487752/1‑99 (MQ=255)

        tAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCt                                                                                            >  2:3031438/1‑99 (MQ=255)

        tAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑AAAAAAAGCCCCTCAATCGAGGGGCtt                                                                                           <  2:494528/99‑2 (MQ=255)

         aaGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTg                                                                                           <  1:1120622/99‑1 (MQ=255)

          aGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTgg                                                                                          <  1:1475839/99‑1 (MQ=255)

          aGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTgg                                                                                          >  1:834941/1‑99 (MQ=255)

          aGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCttt                                                                                          <  1:840289/99‑3 (MQ=255)

          aGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCttt                                                                                          <  2:2243355/99‑3 (MQ=255)

          aGGGCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTgg                                                                                          <  2:1898446/99‑1 (MQ=255)

            gTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGa                                                                                        >  1:3139353/1‑99 (MQ=255)

             tCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGaa                                                                                       <  2:168379/99‑1 (MQ=255)

               gTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATg                                                                                     >  2:393778/1‑99 (MQ=255)

                tttATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgg                                                                                    <  1:3063442/99‑1 (MQ=255)

                tttATGGAAATGATAGGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgg                                                                                    >  2:1682793/1‑99 (MQ=255)

                  tATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGt                                                                                  <  1:1934535/99‑1 (MQ=255)

                    tGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGa                                                                                <  1:2333527/99‑1 (MQ=255)

                    aggAAATGATATGTATCTAATATAGCGGATTAGCCACCTTTTTTCTCTTGAAAAAAAAAAAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGa                                                                                <  2:586447/98‑1 (MQ=255)

                     ggAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAt                                                                               >  1:3539428/1‑99 (MQ=255)

                      gAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATc                                                                              <  1:3663882/99‑1 (MQ=255)

                       aaaTGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCa                                                                             >  1:2155951/1‑99 (MQ=255)

                       aaaTGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCa                                                                             >  2:2166211/1‑99 (MQ=255)

                         aTGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAAc                                                                           <  1:1691051/99‑1 (MQ=255)

                          tGATATGTTTCTGTAATAAAGGATTATGAATATTTTATGTCCTGACGCCTGGAAAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACg                                                                          <  2:855096/99‑1 (MQ=255)

                           gATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACgg                                                                         <  1:2531440/99‑1 (MQ=255)

                             tatGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAACAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGga                                                                       >  2:330833/1‑99 (MQ=255)

                             tatGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGga                                                                       <  1:2912033/99‑1 (MQ=255)

                                gTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaaga                                                                    <  1:2581090/99‑1 (MQ=255)

                                   tCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaa                                                                 >  1:415575/1‑99 (MQ=255)

                                   tCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaa                                                                 >  2:317580/1‑99 (MQ=255)

                                    atGATATAGCGGATTAGCCAGCGTTTATCTCATGAAAAAAAAAAAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaaa                                                                <  2:265511/98‑1 (MQ=255)

                                         ataGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCa                                                           <  1:2961461/99‑1 (MQ=255)

                                         ataGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCa                                                           <  1:3555547/99‑1 (MQ=255)

                                          taGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAg                                                          <  1:1894741/99‑1 (MQ=255)

                                              ggATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCa                                                      >  2:3796278/1‑99 (MQ=255)

                                              ggATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCa                                                      <  1:2365608/99‑1 (MQ=255)

                                               gATTAGCCATCTTTTATCTCCTGACGCCGGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAg                                                     <  2:1876631/99‑1 (MQ=255)

                                                    gacATCTTTTATCTCCTGACGCCTTGGCCAAAAAAAGCCCCACGATTAAGGGGCTTGGTAAGGATCATCAACGGGAGGAAAAACGGCAGGCCAGCGTCt                                                >  2:215983/3‑99 (MQ=255)

                                                    gCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCt                                                >  2:187951/1‑99 (MQ=255)

                                                    gCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCt                                                >  1:506660/1‑99 (MQ=255)

                                                      cATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCttt                                              >  1:2656558/1‑99 (MQ=255)

                                                       aTCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCtttt                                             <  1:2870210/99‑1 (MQ=255)

                                                         cTTTTAACTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAACAAAGAAAAAAAAAACGGCAGGCCAGCGTCtttttt                                           <  2:1347187/99‑1 (MQ=255)

                                                          ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCGACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc                                          >  1:1811182/1‑99 (MQ=255)

                                                          ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc                                          <  2:2634634/99‑1 (MQ=255)

                                                          ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc                                          >  1:378571/1‑99 (MQ=255)

                                                            ttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAg                                        >  2:663382/1‑99 (MQ=255)

                                                            ttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAg                                        >  2:10458/1‑99 (MQ=255)

                                                                 tcCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGAcgcg                                   >  1:2941949/1‑99 (MQ=255)

                                                                   cTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGt                                 >  1:2873051/1‑99 (MQ=255)

                                                                   cTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGt                                 <  1:1828780/99‑1 (MQ=255)

                                                                    tGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTa                                >  1:134423/1‑99 (MQ=255)

                                                                        gCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCGGGCCAGCGTCTTTTTTCAGACGCGGTAAGAc                            >  1:3904569/1‑99 (MQ=255)

                                                                        gCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGAc                            >  1:676196/1‑99 (MQ=255)

                                                                           tGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACaaa                         >  2:349296/1‑99 (MQ=255)

                                                                           tGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACaaa                         >  2:397125/1‑99 (MQ=255)

                                                                                aaaaaaaaGCCCCTCGATTGATGGGGTTGGTATTGGTGATCAACTGGTAGAAAAGCTGCAGGCCAGCGTCGTTGGTGATACTCGGTAAGACaaaagggt                    >  2:3087492/1‑95 (MQ=255)

                                                                                aaaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc                    >  2:1769644/1‑99 (MQ=255)

                                                                                aaaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc                    >  1:1581551/1‑99 (MQ=255)

                                                                                aaaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc                    <  1:2436073/99‑1 (MQ=255)

                                                                                aaaaaaaaGCCCCGCGAGTGAGGGGCTGGGAATGGGGGATGAACGGGAAGAAAAACGGCAGGCCAGGGTCTTTTTTCCGACGCGGTAAGACAAGATGTc                    >  2:3655404/1‑99 (MQ=255)

                                                                                 aaaaaaaGCCCCTGGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg                   <  2:2884477/99‑1 (MQ=255)

                                                                                 aaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAAGGGCAGGCCAGCATCTTTTTTCAGACGCGGTAAGACAAAATGTCg                   >  1:3631917/1‑99 (MQ=255)

                                                                                 aaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg                   <  1:3525553/99‑1 (MQ=255)

                                                                                 aaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg                   >  2:380418/1‑99 (MQ=255)

                                                                                  aaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGa                  >  2:2046392/1‑99 (MQ=255)

                                                                                   aaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa                 >  2:298812/1‑99 (MQ=255)

                                                                                    cccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGCCGCGGTAAGACAAAATGGCGCac                >  1:3216215/4‑99 (MQ=255)

                                                                                    cccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAac                >  1:2255851/4‑99 (MQ=255)

                                                                                     ccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca               >  1:1074142/3‑99 (MQ=255)

                                                                                     ccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca               >  1:374007/3‑99 (MQ=255)

                                                                                     ccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAAGGTCGAaca               >  2:1444733/3‑99 (MQ=255)

                                                                                      caGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacac              >  1:2459973/2‑99 (MQ=255)

                                                                                      caGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacac              >  2:2667787/2‑99 (MQ=255)

                                                                                      caGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacac              >  1:69296/2‑99 (MQ=255)

                                                                                      caGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacac              >  2:3504023/2‑99 (MQ=255)

                                                                                      caGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacac              >  1:1655709/2‑99 (MQ=255)

                                                                                       aGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACTCGTTAAAACAAAATGTCGAacaca             >  2:2262898/1‑99 (MQ=255)

                                                                                       aGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca             >  1:1945989/1‑99 (MQ=255)

                                                                                       aGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca             >  1:2012510/1‑99 (MQ=255)

                                                                                       aGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca             >  1:3666494/1‑99 (MQ=255)

                                                                                       aGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca             >  1:1767017/1‑99 (MQ=255)

                                                                                       aGCCCCTCAATCGAGGGGCTGGGAATGGGAGATCAACGGGAAGAAACACGGCAGGCCAGCGTATTTTCTCAGACGCGGTAAGACACAACGTCGAacaca             >  1:334220/1‑99 (MQ=255)

                                                                                        gCCCCTCTATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacacac            <  2:2709665/99‑1 (MQ=255)

                                                                                        gCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacacac            >  1:2642960/1‑99 (MQ=255)

                                                                                            cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGaa        >  1:1733684/1‑99 (MQ=255)

                                                                                            cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TCTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAAc       <  1:3429038/99‑1 (MQ=255)

                                                                                            cTCGATTGAGGGGCTGGGAAGGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGaa        <  2:1216517/99‑1 (MQ=255)

                                                                                            cTCGATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGCCGAACACCCTgca        >  2:791060/1‑97 (MQ=255)

                                                                                               gATTGAGCGGCTGGGAATGGGTGATCAACGGGAAGAAACACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATCTCGAACACACTGAACCa     <  2:772616/99‑1 (MQ=255)

                                                                                                aTTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCAt    >  1:2275129/1‑99 (MQ=255)

                                                                                                aTTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCAt    <  2:1750384/99‑1 (MQ=255)

                                                                                                aTTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCAt    <  2:1561470/99‑1 (MQ=255)

                                                                                                aTTGAGGGGCTGGGAATGGGCGATCAACGGGAAGAAAAACGGCAGGCCAGAGTC‑TTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa   <  2:810088/99‑1 (MQ=255)

                                                                                                  tGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATAc  <  2:2808295/99‑1 (MQ=255)

                                                                                                  tGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATAc  <  1:1247810/99‑1 (MQ=255)

ATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATAC  >  NC_000913/4472339‑4472535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`