Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,469 T→G intergenic (+49/‑38) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,4690TG99.2% 456.3 / ‑3.4 131intergenic (+49/‑38)ilvL/ilvXilvG operon leader peptide/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (64/66);  minor base . (1/0);  total (65/66)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

TTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAA  >  NC_000913/3950374‑3950564
                                                                                               |                                                                                               
 tattatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGgtttt                                                                                             <  1:2273685/99‑1 (MQ=255)
   ttatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGgtttttt                                                                                           <  1:3001547/99‑1 (MQ=255)
   ttatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGgtttttt                                                                                           <  1:2013731/99‑1 (MQ=255)
    tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGgttttttt                                                                                          <  1:1073361/99‑1 (MQ=255)
    tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGgttttttt                                                                                          <  1:661252/99‑1 (MQ=255)
    tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGgttttttt                                                                                          <  1:3390093/99‑1 (MQ=255)
      tCCGACCGTGCGGAGCTGCACTTGGACGTTGAAAAGCTTAGAGATCAAGCCTTAGCGAACTTTGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGa                                                                                        <  2:892922/99‑1 (MQ=255)
      tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGa                                                                                        <  1:1460494/99‑1 (MQ=255)
       cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGAc                                                                                       >  1:2824923/1‑99 (MQ=255)
         cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCt                                                                                     >  1:1449352/1‑99 (MQ=255)
           ccGTGCCGGGCTGCACGTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTa                                                                                   <  2:2034992/99‑1 (MQ=255)
             gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTaaa                                                                                 >  1:2330689/1‑99 (MQ=255)
             gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTaaa                                                                                 <  1:1370537/99‑1 (MQ=255)
              tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACNCCCGNACCGAAANGTCCGGGGGGTTTTTTTGACCTTaaaa                                                                                <  1:2197787/99‑1 (MQ=255)
              tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTaaaa                                                                                <  1:2022645/99‑1 (MQ=255)
               gCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTaaaaa                                                                               <  2:2521294/99‑1 (MQ=255)
               gCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTaaaaa                                                                               <  1:2524393/99‑1 (MQ=255)
               gCGGGGCTGCACTTGGACGAGCAAACGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTaaaaa                                                                               <  2:3645148/99‑1 (MQ=255)
                cGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAAc                                                                              <  1:519374/99‑1 (MQ=255)
                  gggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACAt                                                                            >  1:1267725/1‑99 (MQ=255)
                   ggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGGTTTTTTGACCTTAAAAACATa                                                                           >  1:3906127/1‑99 (MQ=255)
                    gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATaa                                                                          <  1:1187336/99‑1 (MQ=255)
                    gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATaa                                                                          >  2:655654/1‑99 (MQ=255)
                     cTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAAc                                                                         >  1:730270/1‑99 (MQ=255)
                      tGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAAcc                                                                        >  1:3412680/1‑99 (MQ=255)
                         aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCgag                                                                     >  1:1071256/1‑99 (MQ=255)
                         aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCgag                                                                     <  1:1490033/99‑1 (MQ=255)
                         aCTTGGACGAGGAAAGGCTTAGAGAGCAAGCCTTAACGAACTAAGACCGCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCgag                                                                     <  2:3484394/99‑1 (MQ=255)
                         aCTTGAACGAGCAAAGGCTTAGAGAGCAAGCCTTAACGAACTAAGACCCCCACACCGACAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCgtg                                                                     >  2:599339/1‑97 (MQ=255)
                          cTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCgagg                                                                    <  1:2061030/99‑1 (MQ=255)
                          cTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCgagg                                                                    <  1:3390451/99‑1 (MQ=255)
                           ttGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCgagga                                                                   <  1:1681168/99‑1 (MQ=255)
                           ttGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCgagga                                                                   >  1:3491452/1‑99 (MQ=255)
                            tGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCgaggag                                                                  <  1:689799/99‑1 (MQ=255)
                            tGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCgaggag                                                                  <  1:689715/99‑1 (MQ=255)
                              gACGAGGAAAGGCTTAGAGCTCAAGCCTTAACGAACTAAGACCCCCGCACCGACAGGTCCTGGGGGGCGTATTGACCTTCACGACATAACCGAGGTGcc                                                                >  2:2315632/1‑98 (MQ=255)
                               aCGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg                                                               <  2:1350026/99‑1 (MQ=255)
                                cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                              <  1:3860858/99‑1 (MQ=255)
                                   ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACaa                                                           >  1:1285668/1‑99 (MQ=255)
                                   ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACaa                                                           >  2:328139/1‑99 (MQ=255)
                                    gAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                                          <  2:1057873/99‑1 (MQ=255)
                                    gAAAGGCTAAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCGTAAAAACATAACCGAGGAGCAGACAAt                                                          >  1:2735505/1‑99 (MQ=255)
                                      aaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGa                                                        >  1:2592420/1‑99 (MQ=255)
                                       aGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGaa                                                       <  2:1157514/99‑1 (MQ=255)
                                        ggCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATACCCGAGGAGCAGACAATGAAt                                                      <  2:734718/99‑1 (MQ=255)
                                        ggCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAAt                                                      <  1:1327908/99‑1 (MQ=255)
                                         gCTTAGAGATCAAGCCTTAACGAAGTAAGACCCCCGCACCGAAAGGCCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATa                                                     >  1:3543131/1‑99 (MQ=255)
                                         gCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATa                                                     >  1:843344/1‑99 (MQ=255)
                                         gCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATa                                                     >  1:1439594/1‑99 (MQ=255)
                                           ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                                   >  2:1639273/1‑99 (MQ=255)
                                           ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                                   >  1:1148174/1‑99 (MQ=255)
                                           ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                                   <  2:1741322/99‑1 (MQ=255)
                                           ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc                                                   >  2:2888979/1‑99 (MQ=255)
                                             agagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAg                                                 >  2:2663522/1‑99 (MQ=255)
                                             agagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAg                                                 >  2:3112013/1‑99 (MQ=255)
                                              gagaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc                                                <  1:2661903/99‑1 (MQ=255)
                                               agaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGTTCTTATAAACATAACCGAGGAGCAGACAATGAATAACAGca                                               >  2:241289/1‑99 (MQ=255)
                                               agaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAAGATACCCGAGGAGCAGACATGTAATAACAGca                                               >  2:3020020/1‑99 (MQ=255)
                                                 aTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGGAGACAATGAATAACAGcaca                                             >  2:879340/1‑99 (MQ=255)
                                                    aaGCCTTAACGAACTAAGAGCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGATCTTAAAATCATAATCGAGGAGCAGACAATGAATAACAGCACaaaa                                          <  1:3520482/99‑1 (MQ=255)
                                                     aGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt                                         >  1:12976/1‑99 (MQ=255)
                                                       ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc                                       >  1:3767672/1‑99 (MQ=255)
                                                       ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGCGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc                                       <  1:3786275/99‑1 (MQ=255)
                                                        cTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt                                      >  2:849943/1‑99 (MQ=255)
                                                         ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg                                     <  1:3650015/99‑1 (MQ=255)
                                                          tAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt                                    <  1:942725/99‑1 (MQ=255)
                                                          tAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt                                    >  2:499278/1‑99 (MQ=255)
                                                          tAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt                                    <  1:2964017/99‑1 (MQ=255)
                                                           aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGtt                                   >  2:255960/1‑99 (MQ=255)
                                                           aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGAAGACAATGAATAACAGCACAAAATTCTGtt                                   >  2:731000/1‑99 (MQ=255)
                                                             cGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                                 >  2:1575911/1‑99 (MQ=255)
                                                             cGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                                 <  2:2119830/99‑1 (MQ=255)
                                                              gAACTAATACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct                                <  2:2764201/99‑1 (MQ=255)
                                                              gAACTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct                                <  2:1823036/99‑1 (MQ=255)
                                                                aCTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                              >  1:770020/1‑99 (MQ=255)
                                                                 cTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATATCAGCACAAAATTCTGTTTCTCaa                             <  1:1197661/99‑1 (MQ=255)
                                                                 cTAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCaa                             >  1:556945/1‑99 (MQ=255)
                                                                  tAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAg                            >  2:1691389/1‑99 (MQ=255)
                                                                  tAAGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAg                            >  2:429380/1‑99 (MQ=255)
                                                                   aaGACCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGa                           <  1:1874160/99‑1 (MQ=255)
                                                                      aCCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc                        >  2:791956/1‑99 (MQ=255)
                                                                      aCCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc                        <  2:3615554/99‑1 (MQ=255)
                                                                      aCCCCCGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc                        <  1:792229/99‑1 (MQ=255)
                                                                        ccccGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAGTTCTGTTTCTCAAGATGCAg                      <  2:3580824/99‑1 (MQ=255)
                                                                        ccccGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg                      <  1:2686725/99‑1 (MQ=255)
                                                                        ccccGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCCCAAGATTCAg                      >  1:307735/1‑99 (MQ=255)
                                                                           cGCACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGAc                   <  2:3417959/99‑1 (MQ=255)
                                                                             cACCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgg                 <  1:1502576/99‑1 (MQ=255)
                                                                              aCCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGATAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTGAGGACggg                >  1:2201959/1‑99 (MQ=255)
                                                                              aCCGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACggg                <  1:2332308/99‑1 (MQ=255)
                                                                               ccGAAAGGTCCGGGGGGTTTTTTGGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAGTCTGTTTCTCAAGATTCAGGACgggg               <  2:2858293/99‑1 (MQ=255)
                                                                                cGAAAGGTCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGa              >  1:865716/1‑99 (MQ=255)
                                                                                      gTCCGGGGGGCTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaac        >  1:1978581/1‑99 (MQ=255)
                                                                                       tCCGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaact       >  1:3294269/1‑99 (MQ=255)
                                                                                         cGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTAt     <  2:708678/99‑1 (MQ=255)
                                                                                         cGGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTAt     <  1:2123070/99‑1 (MQ=255)
                                                                                         cGGGGGGATTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCCAGATTCAGGACGGGGAACTAACTAt     >  2:3511187/1‑99 (MQ=255)
                                                                                           ggggGTTTTTTTGACCTTAAAAACATATCCGAGGTGCAGACAATGAATAACAGCACAAAATTCTGTTTCTTAAGATTCAGGACGGGGAACTAACTATGa   <  2:2003717/99‑1 (MQ=255)
                                                                                           ggggGTTTTTTTGACCTTAAAAACATAAACGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTGCTAAAGATTCAGGACGGGAAACTAACTATGa   <  2:2751824/99‑1 (MQ=255)
                                                                                            ggggTTTTATTGACCTTAAAAACATAACCGAGGAGTAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGATGGGGAACTAACTATGaa  <  2:3242424/99‑1 (MQ=255)
                                                                                               |                                                                                               
TTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAA  >  NC_000913/3950374‑3950564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: