Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,777,977 A→T intergenic (+195/+169) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,777,9770AT98.6% 461.5 / ‑1.9 139intergenic (+195/+169)ypjF/ypjACP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (62/75);  total (63/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.67e-01

Only 100 of 139 total aligned reads displayed.

TTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGAC  >  NC_000913/2777881‑2778071
                                                                                                |                                                                                              
ttttCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGc                                                                                              <  1:1621432/99‑1 (MQ=255)
ttttCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGc                                                                                              <  2:2039220/99‑1 (MQ=255)
 tttCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGcc                                                                                             <  1:2229721/99‑1 (MQ=255)
   tCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCcca                                                                                           <  2:2207882/99‑1 (MQ=255)
     ttttAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCccacc                                                                                         >  1:3916536/1‑99 (MQ=255)
       ttAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCaa                                                                                       >  2:169741/1‑99 (MQ=255)
       ttAGGACCAACAATGGGACCAAAATGAAAATTGAACTAAAGATTACAAGCAGATTAACAACAAGTTACACAACCAATTCAGACTCCGCCTACCCACCaa                                                                                       >  2:1737617/1‑99 (MQ=255)
        tAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAAt                                                                                      >  1:1150675/1‑99 (MQ=255)
         aGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATc                                                                                     <  2:2577796/99‑1 (MQ=255)
          ggACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCa                                                                                    >  2:1262264/1‑99 (MQ=255)
           gaacaacaaTGAGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCAt                                                                                   <  2:2406724/96‑1 (MQ=255)
           gACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCAt                                                                                   <  2:3669942/99‑1 (MQ=255)
           gACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCAt                                                                                   >  2:215043/1‑99 (MQ=255)
           gACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCAt                                                                                   <  1:84977/99‑1 (MQ=255)
             ccAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGa                                                                                 >  1:2563766/1‑99 (MQ=255)
             ccAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGa                                                                                 <  2:3800927/99‑1 (MQ=255)
              caacaaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGAt                                                                                <  1:2048839/99‑1 (MQ=255)
               aacaaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGAtt                                                                               <  1:3044541/99‑1 (MQ=255)
               aacaaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGAtt                                                                               >  1:2171387/1‑99 (MQ=255)
                acaaTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTg                                                                              >  2:3056256/1‑99 (MQ=255)
                acaaTGGGACCAAAAGGAAAATTGAACTGAAGATTATAATCAAATTAAAAAAAACTTAAACAACCAATTCAGACTCCGCCTGCCCACCAATCACAATTg                                                                              >  2:114916/1‑99 (MQ=255)
                   aTGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGAc                                                                           <  2:3205056/99‑1 (MQ=255)
                    tGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACg                                                                          <  1:2666389/99‑1 (MQ=255)
                    tGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACg                                                                          <  1:3898803/99‑1 (MQ=255)
                        aCCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGgtgt                                                                      >  2:1629202/1‑99 (MQ=255)
                          cAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTaa                                                                    <  2:2399417/99‑1 (MQ=255)
                          cAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTaa                                                                    <  1:231661/99‑1 (MQ=255)
                          cAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTaa                                                                    <  1:1801617/99‑1 (MQ=255)
                           aaaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAg                                                                   <  1:2754728/99‑1 (MQ=255)
                           aaaaTGAAAATTGAACTGAAGAGCACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTACGTCTGCCCACCGATCATGATTGGATGGTTTaaa                                                                   >  2:2307515/1‑98 (MQ=255)
                            aaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAgg                                                                  >  2:1032267/1‑99 (MQ=255)
                                gagaaTTGGACTGAAGATTACAATCCATTCAACAACAAGTTACACAACCGATTCAGATTCCCCATGCCCACTAATTATGATGCGACGGAGTAAGTacaa                                                              <  2:2599239/96‑1 (MQ=255)
                                gAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGacaa                                                              >  2:723870/1‑99 (MQ=255)
                                gAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGacaa                                                              >  2:2411911/1‑99 (MQ=255)
                                gAAAATTGAACTGAAGATTACAAGCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGacaa                                                              <  2:2713575/99‑1 (MQ=255)
                                 aaaaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGacaac                                                             <  1:2468959/99‑1 (MQ=255)
                                 aaaaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGacaac                                                             <  1:1603057/99‑1 (MQ=255)
                                   aaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAacac                                                           <  1:3558185/99‑1 (MQ=255)
                                   aaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAacac                                                           <  1:3558210/99‑1 (MQ=255)
                                      tGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACcaa                                                        <  1:1238600/99‑1 (MQ=255)
                                       gAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACcaac                                                       <  1:123392/99‑1 (MQ=255)
                                       gAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACcaac                                                       <  1:3704823/99‑1 (MQ=255)
                                             aaGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAAc                                                 <  2:1962873/99‑1 (MQ=255)
                                             aaGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAAc                                                 <  1:3904630/99‑1 (MQ=255)
                                             aaGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAAc                                                 >  2:2894169/1‑99 (MQ=255)
                                              aaaTTACAATAAATCTAACCACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACa                                                <  2:1926808/97‑1 (MQ=255)
                                                 ttACAATCAATTTAACAACAAGTTACACAACCAATTCAGAGTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAATCAGGa                                             >  1:3758990/1‑99 (MQ=255)
                                                  tACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGaa                                            <  2:2982857/99‑1 (MQ=255)
                                                    caatcaatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGt                                          <  1:1740781/99‑1 (MQ=255)
                                                     aatcaatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGtt                                         >  2:1438748/1‑99 (MQ=255)
                                                     aatcaatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGtt                                         >  1:2053951/1‑99 (MQ=255)
                                                      atcaatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTa                                        <  2:1034044/99‑1 (MQ=255)
                                                      atcaatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTa                                        >  1:558223/1‑99 (MQ=255)
                                                      atcaatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTa                                        >  2:3430054/1‑99 (MQ=255)
                                                       tcaaaTAAACAACAAGAAACACAACCAATACAGACTCCGCCTGCCCACCAATCATGAGTGGACGGTCTAAGAAAAAAAAAAAAAAAAACAGGAAGTTAg                                       <  2:3186176/99‑1 (MQ=255)
                                                        caatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGa                                      >  1:306395/1‑99 (MQ=255)
                                                         aatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTAGACGGTGTAAGGACAACACCACCAAAAACAGGAAGTTAGaa                                     >  2:3177983/1‑99 (MQ=255)
                                                          atTTAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAg                                    >  1:2066962/1‑99 (MQ=255)
                                                           tttAACAACAAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGt                                   >  2:2657557/1‑99 (MQ=255)
                                                               acaacaAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCa                               <  1:3060134/99‑1 (MQ=255)
                                                               acaacaAGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCa                               <  1:3553166/99‑1 (MQ=255)
                                                                caacaaGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAg                              <  1:3182024/99‑1 (MQ=255)
                                                                 aacaaGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGc                             >  1:825947/1‑99 (MQ=255)
                                                                 aacaaGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGc                             >  2:1447148/1‑99 (MQ=255)
                                                                  acaaGTTACACAACCAATTCAGACTCCGCCTGCCAACAAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTGTCAGCa                            >  1:852499/1‑99 (MQ=255)
                                                                    aaGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCaaa                          >  1:2135411/1‑99 (MQ=255)
                                                                    aaGTTACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGAAAAAAAAAAAAAAAACAGGAAGTTAGAAGTCTCAGCaaa                          <  2:1420763/99‑1 (MQ=255)
                                                                        tACACAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAacac                      <  1:2772959/99‑1 (MQ=255)
                                                                         acacAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACAcc                     >  1:466314/1‑99 (MQ=255)
                                                                         acacAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACAcc                     >  2:3132588/1‑99 (MQ=255)
                                                                         acacAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACAcc                     >  1:759500/1‑99 (MQ=255)
                                                                         acacAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACAcc                     <  1:1417578/99‑1 (MQ=255)
                                                                         acacAACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACAcc                     >  2:3130858/1‑99 (MQ=255)
                                                                          cacaACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCg                    <  2:2599161/99‑1 (MQ=255)
                                                                          cacaACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCg                    <  1:1943893/99‑1 (MQ=255)
                                                                           acaACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGa                   <  1:1392124/99‑1 (MQ=255)
                                                                           acaACCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGa                   >  1:1121179/1‑99 (MQ=255)
                                                                             aaCCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGAcc                 >  1:1049970/1‑99 (MQ=255)
                                                                             aaCCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGAcc                 >  1:63107/1‑99 (MQ=255)
                                                                             aaCCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGAcc                 >  1:1026934/1‑99 (MQ=255)
                                                                              aCCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCa                <  1:2846795/99‑1 (MQ=255)
                                                                              aCCAATTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCa                >  1:3265271/1‑99 (MQ=255)
                                                                                 aaTTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGAc             >  2:274631/1‑99 (MQ=255)
                                                                                  aTTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACg            <  1:1359249/99‑1 (MQ=255)
                                                                                  aTTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACg            <  2:520507/99‑1 (MQ=255)
                                                                                  aTTCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACg            <  1:866289/99‑1 (MQ=255)
                                                                                   ttCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACAACAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACgg           >  2:1734567/1‑99 (MQ=255)
                                                                                    tCAGACTCCGCCTGCCCACCAGTCATGGTTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGt          <  1:3960281/99‑1 (MQ=255)
                                                                                    tCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGt          <  2:58426/99‑1 (MQ=255)
                                                                                    tCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGt          <  1:2289702/99‑1 (MQ=255)
                                                                                    tCAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGt          <  2:3296956/99‑1 (MQ=255)
                                                                                     cAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTg         >  1:3421564/1‑99 (MQ=255)
                                                                                     cAGACTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTg         >  1:1890440/1‑99 (MQ=255)
                                                                                      aGACTCCGCCTGCCCACCACTCAAGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTga        <  2:1183839/99‑1 (MQ=255)
                                                                                        aCTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTgagg      <  2:1567076/99‑1 (MQ=255)
                                                                                         cTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTgagga     <  1:282305/99‑1 (MQ=255)
                                                                                         cTCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTgagga     <  1:589734/99‑1 (MQ=255)
                                                                                          tCCGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTgaggag    >  1:1430254/1‑99 (MQ=255)
                                                                                            cGCCTGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGAc  <  1:1394645/99‑1 (MQ=255)
                                                                                            cGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGAc  <  1:174093/99‑1 (MQ=255)
                                                                                                |                                                                                              
TTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGAC  >  NC_000913/2777881‑2778071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: