| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,159,089 | 0 | T | A | 26.2% | 91.0 / 13.2 | 42 | L235Q (CTA→CAA) | mdtC | multidrug efflux system, subunit C |
| Reads supporting (aligned to +/- strand): ref base T (25/6); new base A (0/11); total (25/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.89e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCG > NC_000913/2158856‑2159324 | cGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGACCCAGACCAATGATGAGCTaaaa > 1:1415254/1‑238 (MQ=255) aGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTCTTCACTACAACAACGGCGGCGCGGTACggcctggcg > 2:331646/1‑231 (MQ=255) cTCGCTCCCGGCCTTGCGCGTCGGGCGGACCCCGCGGCCGCTTTTAAACCAGGGCGTGCCGGGGGCCGACG‑TACCCACCGCCGGCAGAAATGCCAA‑CGGGCGAAACCCGCGGGGC‑GCG‑GTGG‑AAGATGGCCCTCACCGCGGGCAGATCCAGACAAAGGAGGAGCAAAAAACCGCCGCTGAATATCAGCCGTTGattatt < 2:1192337/199‑1 (MQ=255) cggcccggccgTACGGGGCGGGAGGACGCCGAAGGCGCGTTTTAACCCGGGTGTGTCGTGGGACGAGG‑GCCGCCCCGCCGTAAGACAGGCCAC‑GGGGCGAAAACCGCAGGG‑‑GCG‑GTGGAAAGTGGGCCCTCCCCCGGGGCAGATCCAGACAAATAAGGAGCAAAAACCCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGa < 2:290780/232‑1 (MQ=255) cccGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCCGGGCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTggtgtacgt > 2:382245/1‑232 (MQ=255) gccgTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTacaaca > 1:1192337/1‑199 (MQ=255) gccgTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCCCGGTTCGCCTGGGCGATGTGGCGACGGTgg > 1:668311/1‑237 (MQ=255) gggCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCa > 1:412449/1‑239 (MQ=255) gtGAATCCGCGGGCGTTTTTTAATGGGGGCGTGGCGCTGGGCGGGG‑CACGCACCGCCGGCAGCAA‑GCCAAGCGGGCTAAACCCGAAGGGC‑GCG‑GTGG‑AAGGTGGCCCTCCCCGGTGGAAGACCCAGACCACGGATGCGCAAAAAGCCGCCGCGGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAgg < 2:822484/237‑1 (MQ=255) cgcTGTTTAATCAGGGCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGACCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGGTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTg > 1:388030/1‑221 (MQ=255) cTGTTTAATCAGGGCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGGGACCGACTCAGTGCAggg > 1:356677/1‑223 (MQ=255) aggcggggCGTGTCGCTGG‑CGCCGATCCGCGCGCCCGCCAGCAATCCCAG‑CGTGAGT‑AACCGCCGGGG‑CCGCCTGA‑AGGAGGGCCCTCCCCGCTGGCAGATCCAACCCAATGAGGAGATAAAAGCCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGAc < 2:970544/234‑1 (MQ=255) aatgggggCGTGCCGCTGGCCGACT‑TCCGCCCCGCCGCCAGCAATGCAAA‑CGGGCATAAACCGCAGGGC‑GCG‑CGGG‑AAGAGGGCCCTCCCCGCTGGCAGATCCAGACCAATGATGAGCAAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTggg < 2:1045178/180‑1 (MQ=255) gggCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGGGGCGACGGGGACCGa > 1:1108147/1‑201 (MQ=255) gCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAAACGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGGGACCGACTAAGTGCAGGATGTGCGCAAACCCGGGATGACCAACGcc > 1:145212/1‑239 (MQ=255) tgtCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCg > 1:1045178/1‑185 (MQ=255) ggtCGCGGGACGGCG‑TACGCCCCGCCGGCAGCAATGCCAA‑CGGGCGTAAACCGGAGGGC‑GCG‑GTGG‑AAGATGGCACCCACCGCTGGCAGACCCAGACAAATGATGAACAAAAAACCGCCGCTGAATATCAGCCGTTGattatt < 2:503002/142‑1 (MQ=255) ggACGACG‑TACGCACCGCCGCCAGAAATGCCAA‑CGTGCGTAACCCGAAGGGC‑GCG‑CTGG‑AAGAGGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCAAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTa < 1:382245/239‑1 (MQ=255) cgacg‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTacaaca > 1:503002/1‑143 (MQ=255) cg‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGAt > 1:848401/1‑196 (MQ=255) tACGCACCGCCGTCGGCAATGCCAA‑CGTGCGCAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTAtttt > 1:1229061/1‑235 (MQ=255) cacaaaTGCCAA‑CGTGCGTAACCCGCGGGGC‑GCG‑CTGG‑AAAATGGCCCTCCCCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAAc < 1:988581/235‑1 (MQ=255) tGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGAGGACCAACGCCAAACCGGCCATTTTACTGATGATCCGCAAACTGCCgg > 1:504649/1‑239 (MQ=255) acggtgctacaCCGGAGGGCGGCG‑TTGG‑AAGAGGACACTAACCGATGGCAGTCCCTGACCAAGGATGGGAAAAAAGCCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGc < 2:668311/229‑1 (MQ=255) tGCGTAAACCGATGGGC‑GCT‑CGGA‑AAGAGTGCCCTCCCCGCTAGCAGATCCATACCACGTATTAGCTAAAACCCGCCGCTGAATATCGGCCTTTGATTATTCACTTCACCAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTTATGATCCGCAAACTGCCGGAAGCCAat < 2:406794/239‑1 (MQ=255) gCGTAACCCGCGGGGC‑GGT‑TTGA‑AAGATGGCCCCCCCCGCTGGCAGCTCCACACCAATGATGAGCAAAAAACCGCCGCTGAATATCAGCCGTTGattatt < 2:471256/100‑1 (MQ=255) tAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAAtatta > 1:94233/1‑239 (MQ=255) gaggggC‑CCG‑CTGG‑AAGATGCCCCTCCCCGCGGGCAACTCCACACCAATGATGGGCAAAAACCCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGa < 1:1301358/236‑1 (MQ=255) cgggggg‑GCG‑CGGG‑AGGAGGGCCCCCCCCGCTGGCAGATCCAGACCAATGATAAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGa < 1:1177271/236‑1 (MQ=255) cAGGGC‑GCG‑CTGG‑AAGATGGCACTCACAGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCAcaacaaaa > 1:471256/1‑98 (MQ=255) ggCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTc < 1:280433/128‑1 (MQ=255) ggCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTc > 2:280433/1‑128 (MQ=255) tCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAg > 1:326292/1‑239 (MQ=255) tCCAGACCAATGAGGGGCAAAAACCCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAg < 1:1324332/239‑1 (MQ=255) acacagagtggtgaGCAAAAACCCGGCGCGTAATATCGGCCGGTGATTATTCACTACAACAGCGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTGTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGt < 2:847755/221‑1 (MQ=255) atgatgAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTggg > 2:100860/1‑76 (MQ=255) atgatgAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTggg < 1:100860/76‑1 (MQ=255) atgatgAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGCAGCCAATATTATCCAGACGGATGACAGCACCCGGGCAAAAATACCGGAGCTGCAGGAAACCAt > 1:252080/1‑238 (MQ=255) atgatgAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGGCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCggg > 1:247421/1‑210 (MQ=255) tgatgaGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGAAAGCATCCGGGCACAATTACCGGGGTTGCAGGAAAc > 2:697308/1‑234 (MQ=255) aGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCACTATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCcggcg > 1:417246/1‑239 (MQ=255) aGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCcggcg > 2:351368/1‑239 (MQ=255) | CGCCTCGACGCAGCTGGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACG‑TACGCACCGCCGTCAGCAATGCCAA‑CGTGCGTAAACCGCAGGGC‑GCG‑CTGG‑AAGATGGCACTCACCGCTGGCAGATCCAGACCAATGATGAGCTAAAAACCGCCGCTGAATATCAGCCGTTGATTATTCACTACAACAACGGCGGCGCGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCG > NC_000913/2158856‑2159324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |