Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,809,761 G→T E49* (GAA→TAA)  ygaZ → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,7610GT98.0% 488.6 / ‑0.8 147E49* (GAA→TAA) ygaZputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (57/87);  total (59/88)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 155 total aligned reads displayed.

GAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCA  >  NC_000913/2809665‑2809856
                                                                                                |                                                                                               
gttggATGCAAAGACCGTTCCCCGATTGTGAGTAGTGATATGCCGGCGGCCTTTGCGTTCTGTCTGCATGCGACCCGTCTGGGATTCTCTCCTCTCTaa                                                                                               <  2:3621006/96‑1 (MQ=255)
gAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTaa                                                                                               <  2:1412082/99‑1 (MQ=255)
 attgATGCAAACACAGTTTACCGATTTTTATTAGTTATATTCCGGTTTCCTTTGCGTTCGGTCTTAATGCGACCCGTCTGTGATTCTCTCCTCTCTaaa                                                                                              <  2:1625805/96‑1 (MQ=255)
 aaGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTaaa                                                                                              <  2:1515388/99‑1 (MQ=255)
 aaGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTaaa                                                                                              <  1:4074699/99‑1 (MQ=255)
 aaGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTaaa                                                                                              >  1:1575079/1‑99 (MQ=255)
 aaGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTaaa                                                                                              <  1:2425426/99‑1 (MQ=255)
 aaGGATGCAAAGACAGTTTAACGATTGGTATTAGTTATACTCCGGTGGCCTTTGCGTTCGGACTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTaaa                                                                                              <  2:755295/99‑1 (MQ=255)
   ggATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGc                                                                                            <  1:1415811/99‑1 (MQ=255)
      tGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGtt                                                                                         >  2:2108820/1‑99 (MQ=255)
       gCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttt                                                                                        <  2:2521988/99‑1 (MQ=255)
       gCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttt                                                                                        >  2:4022870/1‑99 (MQ=255)
       gCAAAGACAGTTTACCGATTGTTAGTAGGTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttt                                                                                        >  2:570306/1‑99 (MQ=255)
        cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGtttt                                                                                       >  1:2229274/1‑99 (MQ=255)
         aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttttt                                                                                      <  1:1176506/99‑1 (MQ=255)
           aGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttttttt                                                                                    >  2:3847193/1‑99 (MQ=255)
           aGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGttttttt                                                                                    <  1:2554194/99‑1 (MQ=255)
            gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTtc                                                                                   <  1:632588/99‑1 (MQ=255)
            gACAGTTTACCGATTGTTATTAGTTATACTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTtc                                                                                   <  1:3752804/99‑1 (MQ=255)
             aCAGTTTACCGATTGTTATTAGTTATATTTCGGTGGCCTTTGCGTTCGGTCTGCATGCGACCCGTCTGGGATTATCTCCTCTCTAAAGCGGTTTTTtct                                                                                  >  2:702116/1‑99 (MQ=255)
             aCAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTtct                                                                                  <  1:2206232/99‑1 (MQ=255)
              cAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTtctc                                                                                 <  1:2239298/99‑1 (MQ=255)
              cAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTtcgc                                                                                 <  1:1936345/99‑3 (MQ=255)
               aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTcc                                                                                <  1:955411/99‑1 (MQ=255)
                gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCt                                                                               >  2:147858/1‑99 (MQ=255)
                gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTCGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCt                                                                               <  1:835149/99‑1 (MQ=255)
                  ttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGc                                                                             <  2:2803603/99‑1 (MQ=255)
                  ttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGc                                                                             <  2:2196901/99‑1 (MQ=255)
                    aCCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGcat                                                                           >  2:3419458/1‑99 (MQ=255)
                      cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGcatca                                                                         >  2:3459173/1‑99 (MQ=255)
                      cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGcatca                                                                         >  1:3175065/1‑99 (MQ=255)
                      cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGcatca                                                                         >  1:565987/1‑99 (MQ=255)
                      cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGcatca                                                                         >  1:3434553/1‑99 (MQ=255)
                        aTTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCttt                                                                       <  1:3184457/99‑1 (MQ=255)
                        aTTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCAtt                                                                       <  1:2589147/99‑1 (MQ=255)
                        aTTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCAtt                                                                       <  1:1807552/99‑1 (MQ=255)
                        aTTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCAtt                                                                       <  2:1553935/99‑1 (MQ=255)
                         atcttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCAttt                                                                      <  1:1847731/96‑1 (MQ=255)
                          tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTa                                                                     >  1:4066420/1‑99 (MQ=255)
                           gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTAt                                                                    <  1:3101146/99‑1 (MQ=255)
                           gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTAt                                                                    <  1:2034052/99‑1 (MQ=255)
                            ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATg                                                                   <  1:411280/99‑1 (MQ=255)
                             tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGc                                                                  <  2:2954911/99‑1 (MQ=255)
                             tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGc                                                                  <  2:516172/99‑1 (MQ=255)
                             tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGc                                                                  >  2:327235/1‑99 (MQ=255)
                               ttaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAg                                                                <  1:2368640/99‑1 (MQ=255)
                               ttaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATCTATGCAg                                                                >  2:2792432/1‑99 (MQ=255)
                                taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATAAATGATGCAgg                                                               <  1:159206/99‑1 (MQ=255)
                                 aGTTATGTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGTTTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATTCTGgc                                                              <  2:3817961/99‑1 (MQ=255)
                                 aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGgc                                                              <  2:2005796/99‑1 (MQ=255)
                                 aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATAAATGATGCAgga                                                              <  2:2946567/99‑2 (MQ=255)
                                 aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATAAATGATGCAgga                                                              <  1:589268/99‑2 (MQ=255)
                                  gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATAAATGATGCAggag                                                             <  2:2043060/99‑3 (MQ=255)
                                  gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATAAATGATGCAggag                                                             <  1:2990249/99‑3 (MQ=255)
                                   ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGgcgc                                                            >  1:1665918/1‑99 (MQ=255)
                                    tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGcgcg                                                           <  1:2206132/99‑1 (MQ=255)
                                     atatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGa                                                          <  1:2632242/99‑1 (MQ=255)
                                     atatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGa                                                          <  1:3292889/99‑1 (MQ=255)
                                     atatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGa                                                          >  1:1350789/1‑99 (MQ=255)
                                      tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAg                                                         <  2:3991385/99‑1 (MQ=255)
                                      tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAg                                                         <  2:2864130/99‑1 (MQ=255)
                                         tCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCa                                                      >  1:417167/1‑99 (MQ=255)
                                         tCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCa                                                      <  2:108246/99‑1 (MQ=255)
                                           cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                    >  1:3968917/1‑99 (MQ=255)
                                             gTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                  >  2:2520129/1‑99 (MQ=255)
                                             gTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                  <  1:1731238/99‑1 (MQ=255)
                                             gTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTc                                                  <  1:816040/99‑1 (MQ=255)
                                              tGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                 <  1:3424035/99‑1 (MQ=255)
                                               ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGt                                                <  1:2898317/99‑1 (MQ=255)
                                                gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCGCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                               <  1:449695/99‑1 (MQ=255)
                                                 ccTTTGCGTTCGGTCTGAATGCGACACGTCTTGGATTCTCTCCTCCCTACAGCGTTTTTTTCTCTTGGATCATTTATGCAGGCGCGAGCAAGTTCGTCa                                              >  2:63543/1‑99 (MQ=255)
                                                  cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                             >  1:3170511/1‑99 (MQ=255)
                                                  cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                             >  1:2879945/1‑99 (MQ=255)
                                                  cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                             >  1:2447410/1‑99 (MQ=255)
                                                  cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                             <  1:11605/99‑1 (MQ=255)
                                                   tttGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt                                            >  2:123756/1‑99 (MQ=255)
                                                          tCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGa                                     <  1:3013369/99‑1 (MQ=255)
                                                              tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCCTTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                 >  1:353086/1‑99 (MQ=255)
                                                              tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                 >  2:3765677/1‑99 (MQ=255)
                                                              tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                 >  2:4040655/1‑99 (MQ=255)
                                                                 gAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                              >  1:3530794/1‑99 (MQ=255)
                                                                 gAATGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                              >  1:3263453/1‑99 (MQ=255)
                                                                  aaTGCGACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTTTCTTTTTTCATTTATGCAGGCGCGAGCCAGTTCGTCATTCCCGCGCTGCTggta                             >  2:1860998/1‑97 (MQ=255)
                                                                       gACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGTCGCGAGCCAGTTCGTCATTACCGCGCTGCTAGCAGCCgg                        >  2:3308967/1‑99 (MQ=255)
                                                                       gACCCGTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                        <  1:2079254/99‑1 (MQ=255)
                                                                            gTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTTTTGCATTATTTATGCAAGCGCGAACCAGTTCTTCTTTACCACGATGCTAGGAGGCTGGagaa                   >  2:610452/1‑97 (MQ=255)
                                                                            gTCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTGATTACGGCGATGCTGGCAGCCGGGagta                   >  1:3757103/1‑99 (MQ=255)
                                                                             tCTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtag                  >  1:697783/1‑99 (MQ=255)
                                                                              cTGGGATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtagt                 <  2:1597896/99‑1 (MQ=255)
                                                                                gggATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt               <  1:4117854/99‑1 (MQ=255)
                                                                                gggATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt               <  1:849346/99‑1 (MQ=255)
                                                                                  gATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt             <  1:459060/99‑1 (MQ=255)
                                                                                  gATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCAAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt             <  1:3203876/99‑1 (MQ=255)
                                                                                  gATTCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTACGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt             <  1:104079/99‑1 (MQ=255)
                                                                                    ttCTCTCCTCTCTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg           <  1:1039226/99‑1 (MQ=255)
                                                                                        ctcctctcTAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtg       <  1:3411993/99‑1 (MQ=255)
                                                                                           ctctctAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg    <  2:1238846/99‑1 (MQ=255)
                                                                                           ctctctAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg    >  1:2666953/1‑99 (MQ=255)
                                                                                           ctctctAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg    <  2:901338/99‑1 (MQ=255)
                                                                                             ctctAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCa  >  1:3807908/1‑99 (MQ=255)
                                                                                                |                                                                                               
GAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCA  >  NC_000913/2809665‑2809856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: