New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4558975 =40 (0.980)6 (0.160) 6/280 2.6 12.3% coding (552/1173 nt) iadA isoaspartyl dipeptidase
?NC_000913 4559021 = 48 (1.250)coding (506/1173 nt) iadA isoaspartyl dipeptidase
Rejected: Frequency below cutoff threshold.

TGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAA  >  NC_000913/4558827‑4559119
                                                                                                                                                    |                                                                                                                                                
cccgtgcgctcaatgaagaaggcatcagcgcctggatgctgaccggcgcttatcatgtcccttcccgcaccattacgggttccgtggaaaaagacgtggcgattatcgatcgtgtgattggcgtgaaatgcgccatctctgatcaccgtt                                                                                                                                                 <  2:187876‑M1/2‑1 (MQ=255)
     ttAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTgccg                                                                                                                                           <  2:500501/151‑1 (MQ=255)
                cGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                <  2:48322/151‑1 (MQ=255)
                        gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATa                                                                                                                        >  1:572101/1‑151 (MQ=255)
                          ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATaaa                                                                                                                      <  2:146938/151‑1 (MQ=255)
                          ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACGGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATaaa                                                                                                                      <  2:82635/151‑1 (MQ=255)
                               gATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTc                                                                                                                  >  1:602813/1‑150 (MQ=255)
                                 tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCACGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCgg                                                                                                               >  1:214869/1‑151 (MQ=255)
                                    gCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTg                                                                                                             >  2:587165/1‑150 (MQ=255)
                                         tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAg                                                                                                       >  2:216234/1‑151 (MQ=255)
                                                 ttCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGa                                                                                               >  1:390674/1‑151 (MQ=255)
                                                        gtcccttcccgcaccattacgggttccgtggaaaaagacgtggcgattatcgatcgtgtgattggcgtgaaaagcgccatctctgatcaccgTTCTGCCGCCATATTGGCCAGGTGATAAACGTCCCGTGCGGCAGAACGGTGATCAgaga                                                                                        <  2:330042‑M1/59‑1 (MQ=255)
                                                         ggTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGAt                                                                                       >  1:383282/1‑151 (MQ=255)
                                                         ggTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGAt                                                                                       >  1:565253/1‑151 (MQ=255)
                                                          gTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCagag                                                                                         <  1:246883/148‑1 (MQ=255)
                                                          gTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAgaga                                                                                        <  2:496824/149‑1 (MQ=255)
                                                          gTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAgaga                                                                                        <  1:589211/149‑1 (MQ=255)
                                                          gTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATg                                                                                      >  2:64421/1‑151 (MQ=255)
                                                          gTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATg                                                                                      >  1:458380/1‑151 (MQ=255)
                                                          gTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATg                                                                                      >  2:42693/1‑151 (MQ=255)
                                                              tAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGgcg                                                                                   >  1:423989/1‑150 (MQ=255)
                                                                     ccattacgggttccgtggaaaaagacgtggcgattatcgatcgtgtgattggcgtgaaatgcgccatctctgatcaccgTTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCa                                                                            <  1:118762‑M1/71‑1 (MQ=255)
                                                                         tAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGcc                                                                        <  2:124559/150‑1 (MQ=255)
                                                                              ccTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATca                                                                   >  2:161683/1‑150 (MQ=255)
                                                                              ccTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATca                                                                   >  1:559645/1‑150 (MQ=255)
                                                                                         tCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATa                                                        <  1:17044/150‑1 (MQ=255)
                                                                                                    gAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGt                                              >  1:452765/1‑149 (MQ=255)
                                                                                                         tcgatcgtgtgattggcgtgaaatgcgccatctctgatcaccgTTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCttttt                                        <  1:1532‑M1/107‑1 (MQ=255)
                                                                                                              aCGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACgg                                  >  2:251567/1‑151 (MQ=255)
                                                                                                               cGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGa                                 >  2:227606/1‑151 (MQ=255)
                                                                                                               cGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGa                                 >  2:328856/1‑151 (MQ=255)
                                                                                                                 ccAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAAc                               <  1:175839/151‑1 (MQ=255)
                                                                                                                      tttACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTa                          <  2:619829/151‑1 (MQ=255)
                                                                                                                       ttACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTaa                         <  2:595219/151‑1 (MQ=255)
                                                                                                                       ttACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTaa                         <  2:441866/151‑1 (MQ=255)
                                                                                                                        gcgtaaaatgcgccatctctgatcaccgTTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAAt                        <  1:99427‑M1/123‑1 (MQ=255)
                                                                                                                          ccgccgAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATgg                      <  1:62334/151‑1 (MQ=255)
                                                                                                                             ccgAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTg                    <  2:499460/150‑1 (MQ=255)
                                                                                                                                      ccGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGa          >  2:350315/1‑151 (MQ=255)
                                                                                                                                       cGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGAc         <  1:330729/151‑1 (MQ=255)
                                                                                                                                       cGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGAc         <  2:423980/151‑1 (MQ=255)
                                                                                                                                       cGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGAc         <  2:345899/151‑1 (MQ=255)
                                                                                                                                         tctgatcaccgTTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACAt       <  2:124369‑M1/140‑1 (MQ=255)
                                                                                                                                            gatcaccgTTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGAt    <  2:389892‑M1/143‑1 (MQ=255)
                                                                                                                                            aCGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGAt    <  1:25016/151‑1 (MQ=255)
                                                                                                                                             cgcgGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATa   <  1:3977/151‑1 (MQ=255)
                                                                                                                                              gcgGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATaa  >  2:57537/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAA  >  NC_000913/4558827‑4559119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.