Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,578,2870TC54.4% 37.0 / 57.8 47E147G (GAA→GGA) gntRd‑gluconate inducible gluconate regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (14/7);  new base C (0/25);  total (14/33)
Fisher's exact test for biased strand distribution p-value = 4.85e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.21e-08

TGATAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAG  >  NC_000913/3578141‑3578427
                                                                                                                                                    |                                                                                                                                            
tgatAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTcca                                                                                                                                           >  2:173207/1‑150 (MQ=255)
  atAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTccacca                                                                                                                                        <  2:492482/151‑1 (MQ=255)
      tACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACggg                                                                                                                                    <  1:432189/151‑1 (MQ=255)
           tcgtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATAc                                                                                                                               <  1:390250/151‑1 (MQ=255)
           tcgtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATAc                                                                                                                               <  2:107534/151‑1 (MQ=255)
                     gTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACtt                                                                                                                      >  2:455655/1‑150 (MQ=255)
                        cgcCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAat                                                                                                                  >  2:252392/1‑151 (MQ=255)
                            gagaTAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAatcat                                                                                                               >  1:632371/1‑150 (MQ=255)
                               aTAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCttt                                                                                                           >  1:479335/1‑151 (MQ=255)
                                    caagggggCGACGCCCGCGAGAAATAAGGGCAGTGGTCATCTGGCGTGCTGCTTAAAAGTAACAAAAACCGACGGCAATATCAAGGCATGGCGACTTGCTGTCCATCAGTCCCCCCACGGGAATACCCGCCACTTCAATCATCTTTAAgg                                                                                                       <  1:190024/144‑1 (MQ=255)
                                    cAATGTGGCGATGCCCGCGAGCAATAAGGGCGGTGGTCATCGGGCGTGCTGCTTAAAATTTATAAAAACCGCCGGCGATATCAAGGCATGGCGACTGGCTGTCCATCAGTCCCACCACGGAAATACCCGCCACTTCAATCATCTTTAAgg                                                                                                       <  1:12664/150‑1 (MQ=255)
                                    cAATGGGGCGATGCCCGCGAGAAATAAGGGAAGTGGTCATCTGGCGTGCTGCTTAAAATTTATAAAACCCGACGGCGATATCAAGGCAGGGCGACTTGCTGTCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAgg                                                                                                       <  1:27183/150‑1 (MQ=255)
                                    aattgtgGCGACGCCCGCGAAAAATAAGGGCAGTGGTCATCGGGCGTGCTGTTTAAAAGTTATCAAACCCGCCGGCGATATAAAGGCAGGGCGTCTTGCTGTCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAgg                                                                                                       <  2:357139/147‑1 (MQ=255)
                                    aatgggggggATGCCCGGGAGAAAAAAGGGCGGGGGTCATCTGGCGTGCTGCTAAAAATTTAAAAAACCCGACGGCGATATCAAGGCAGGGCGTCTTGCTGTCCATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAgg                                                                                                       <  2:62102/141‑1 (MQ=255)
                                    aaaTGTGGCGAGGCCCGCGAGCAATAATGGCGGTGGTCATCGGGGGTGCTGCTTAAAAGTTACAAAACCCGCCGGCGATATAAAGGCAGGGCGCCTTGCTGCCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAgg                                                                                                       <  1:428389/149‑1 (MQ=255)
                                     aaTGGGGCGACGCCCGCGAGAAATAATGGCAGTGGTCTTCTGGCGTGCTGCTTCAAATTTACCAAAACCGACGGCAATATAAAGGCATGGCGACTTGCTTTCCATCAGTCCCCCCCGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTg                                                                                                     <  2:394185/151‑1 (MQ=255)
                                      aTGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTg                                                                                                     >  1:588891/1‑150 (MQ=255)
                                                     cgAGAAATAATGGCGGTGCTCATCTGGCGCGTTTTTACAAAGTCACAAAACCAGACGCCGATAGCAAGGGTGGGGGCTTCGCTTTCTTGCGTTGCCGCCGGGGAAAAACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTa                                                                                      <  1:269352/150‑1 (MQ=255)
                                                         cAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGtt                                                                                  >  2:311843/1‑150 (MQ=255)
                                                         cAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGCGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGtt                                                                                  >  1:465563/1‑150 (MQ=255)
                                                         aaaaaatGGAGGTGTTCATCGGGCGTGCTCCTTAAAATTTATAAAACCCGCCGGCGATATCAGGGCAGGGCGATTTGCTGCCCATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTc                                                                                 <  1:37704/147‑1 (MQ=255)
                                                            taatGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGt                                                                              >  1:377511/1‑151 (MQ=255)
                                                                  cggTGGTCATCTGGCGTGCTGCTTAAAATTTAACAAACCCGAGGGCGATATCAAGGCATGGCGACTTGCTGCCCATCAGTCCCCCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGa                                                                         <  1:84159/148‑1 (MQ=255)
                                                                  aaGTGGTCTTCGGGCGTGCTGCTTCAAATTTATAAAACCCGCCGGCAATATAAAGGCTTGGCGTCTTGCTGTCCATCATTCCCCCCAGGGAAAACCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGa                                                                         <  1:119031/149‑1 (MQ=255)
                                                                       ttattcgggcggcctgctTCAAATTTAAAAAACCCGCCGGCGATACAAGGGCAGGGCGATTTGCTGTCCATCATTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGc                                                                   <  2:259673/138‑1 (MQ=255)
                                                                        tCATCGGGCGTCCTCTTAAAAAGTTACCAAACCCGCCGGCAATAACAAGCCATGGCGTCTTGCTCCCCATCATTCCCCCCCCGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGcc                                                                  <  1:637708/151‑1 (MQ=255)
                                                                         cATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCg                                                                 >  1:39222/1‑151 (MQ=255)
                                                                               gggTGCTGCTTAAAAGTTATCAAACCCGACGGCGATATCAAGGCAGGGCGACTTGCTGTCCATCAGTCCCCCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat                                                            <  1:538113/148‑1 (MQ=255)
                                                                                    ctgttaaaaaTTTATCAACCCCGCGGGGAATATCAGGGAGTGGCGTTTTTTTGCCCATCAGTCCCCCCGGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTcc                                                       <  2:347213/143‑1 (MQ=255)
                                                                                      gctTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAgg                                                    >  1:269301/1‑151 (MQ=255)
                                                                                       cttaaaattaaaaaaacCCGACGGCAATATCAAGGCATGGCGCTTTGCTTTCCATCAGTTCCCCCGGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAgg                                                    <  2:422272/137‑1 (MQ=255)
                                                                                       ctTCAAATTTATCAAAACCGACGGCTATATCAAGGCATGGCGCCTTGCTGCCCATCATTCCCACCACGGGAATCCCCGCCACTTCAGTCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAgg                                                    <  1:216630/150‑1 (MQ=255)
                                                                                        ttaaaattaaacAAACCCGCCGGCGATATAAAGGCATGGCGACTTCCTGCCCATCATTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag                                                  <  2:335659/140‑1 (MQ=255)
                                                                                        ttaaaaGTTATAAAACCCGACGGCGATATCAAGGCAGGGCGACTTGCTGTCCATCAGTCCCCCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag                                                  <  1:152877/148‑1 (MQ=255)
                                                                                        tccAAAGTAACCAAAACCGACGAAAATATAAAGGAATGCCACGTTGCTGTCCTTCTGTCCCGCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag                                                  <  1:71417/149‑1 (MQ=255)
                                                                                        tacAAAGTTAACAAAACCGACGGCGATACCAAGCCATGGCGCCTTGCTGTCCATCTGTCCCCCCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag                                                  <  2:43970/149‑1 (MQ=255)
                                                                                        taaaaatttaaaaaacCCGCCGGCGATATCAAGGCAGGGCGTCTTGCTGTCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag                                                  <  2:611118/139‑1 (MQ=255)
                                                                                                       cccGACGGCGATATCAAGGCATGGCGCCTTGCTGCCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAgg                                   <  1:173210/150‑1 (MQ=255)
                                                                                                                   tatCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCt                         >  1:402241/1‑150 (MQ=255)
                                                                                                                   tatCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCt                         >  1:130840/1‑150 (MQ=255)
                                                                                                                   tatCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCt                         >  2:425368/1‑150 (MQ=255)
                                                                                                                                cgttttGCTGTCCATCATTTCCCCCACGGGAACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGttt            <  1:74510/146‑1 (MQ=255)
                                                                                                                                 ctattggCTGCCCATCAGTTCCACCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGttt            <  1:162935/144‑1 (MQ=255)
                                                                                                                                    ttGCTGTCCATCATTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAc       <  1:153930/151‑1 (MQ=255)
                                                                                                                                     tggcgctCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAc       <  1:154311/145‑1 (MQ=255)
                                                                                                                                        cTGCCCATCATTCCCCCCAGGGAAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCg     <  1:58036/150‑1 (MQ=255)
                                                                                                                                           tCCATCGGTCCCACCACGGAAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAg  <  1:538100/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
TGATAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAG  >  NC_000913/3578141‑3578427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: