Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,9040AC53.8% 29.7 / 35.5 26G47G (GGT→GGG) ssuBaliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (8/4);  new base C (0/14);  total (8/18)
Fisher's exact test for biased strand distribution p-value = 3.17e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-04

CCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAC  >  NC_000913/993756‑994046
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ccGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCcaccac                                                                                                                                               >  1:267935/1‑150 (MQ=255)
            gTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCcca                                                                                                                                   >  2:183286/1‑150 (MQ=255)
            gTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCcca                                                                                                                                   >  1:440385/1‑150 (MQ=255)
                   aCGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTggctgaaattcaggaa                                                                                                                           <  1:571181/151‑16 (MQ=255)
                          tCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACaaa                                                                                                                      >  1:58028/1‑149 (MQ=255)
                                         cgcgTGTCTTCCTGAATTTCAGCCGGCGGTGGGGGGCCCGCTAACACACCGCCGGCGGTTGGCTTTCCCAGCCCGGCCAGCAGGCGCAGCAGGGTACTTTTGCCCCCCCCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAatat                                                                                                     <  1:232257/151‑1 (MQ=255)
                                                ctcccTAAATTCCACCCGGCGGTGGGGCCCCCGCAACCACCCCCCCGGGGGTGGGCGTTCCCAGCCCTCCCAGCAGCCGCAGCGGGTTACTTTTGCCACCCCGGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATc                                                                                              <  2:155031/148‑1 (MQ=255)
                                                 ttCCGGATTTCCAGCCAGCGGTGGGGCCCCCGCTAACACCCCGCCTGGGGTTGGCTTTCCCAGCCCCGCCAGCAGGCCCAGAAGGTTCCTTTCCCCACCCCCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATcc                                                                                             <  2:456322/151‑1 (MQ=255)
                                                      gAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTg                                                                                        >  1:72105/1‑151 (MQ=255)
                                                                  gcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGat                                                                            >  1:520614/1‑151 (MQ=255)
                                                                          gtgCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcg                                                                    >  1:193395/1‑151 (MQ=255)
                                                                                        cccGCCTGCGGTTGGCTTTCCCAGCCCCGCCAGCAGGCGCAGAAGGGTATTTTTCCCCCCCCCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCtt                                                      <  2:507483/149‑1 (MQ=255)
                                                                                        cccGCCGGCGTTGGGCGTTCCCAGCCCTGCCAGCAGGCGCAGCAGGTTACTTTTGCCACCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCtt                                                      <  2:102187/149‑1 (MQ=255)
                                                                                        aTCGCCGGCGGTTGGCGTTCCCAGCCCTGCCAGCAGGCGCAGCAGGGTACTTTTCCCCCCACCGCGGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCtt                                                      <  1:552067/151‑1 (MQ=255)
                                                                                                         ttcccaccccccccAGCAGGCGCAGCAGGGTACTTTCGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaacaa                                     <  2:391590/139‑1 (MQ=255)
                                                                                                          ttCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAAt                                    >  1:524206/1‑151 (MQ=255)
                                                                                                                cccTGCCAGCAGGCGCAGCAGGTTATTTTTGCCCCCCCCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCg                                <  1:24131/148‑1 (MQ=255)
                                                                                                                  cccccAGCGGGCGCAGCGGGTTCCTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGcc                            <  2:609973/148‑1 (MQ=255)
                                                                                                                          agGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCa                    >  2:151487/1‑151 (MQ=255)
                                                                                                                             cgcAGCGGGGTATTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGAc                 <  1:465049/151‑1 (MQ=255)
                                                                                                                             cgcAGCAGGTTACTTTTCCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGa                  <  2:440376/150‑1 (MQ=255)
                                                                                                                             cccagcagGGTTTTTTTCCCCCCCCCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGAc                 <  1:540046/149‑1 (MQ=255)
                                                                                                                                        ttttttCCCCCCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTc      <  1:626000/149‑1 (MQ=255)
                                                                                                                                        cctttcccccccaccGCTCCCGCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTc      <  2:243530/141‑1 (MQ=255)
                                                                                                                                          tttgGCCCCCCCCGCTGCGGCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCAt    <  2:105504/151‑1 (MQ=255)
                                                                                                                                            ttGCCCCCCCCGCGGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc  <  1:549449/151‑1 (MQ=255)
                                                                                                                                            ttGCCACCACCGCTGCGGCCCACCACCACCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc  <  2:24544/151‑1 (MQ=255)
                                                                                                                                            tcgCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc  <  2:522075/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAC  >  NC_000913/993756‑994046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑