Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,364	A→T	L193Q (CTG→CAG)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,364	0	A	T	100.0%	148.3 / NA	40	L193Q (CTG→CAG)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/22);  total (18/22)

GTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAG  >  NC_000913/2907217‑2907510                                                                                                                                                    |                                                                                                                                                   gTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGt                                                                                                                                                 >  1:445283/1‑151 (MQ=255)      gTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATgg                                                                                                                                            >  1:388444/1‑151 (MQ=255)       tCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGa                                                                                                                                           <  2:484945/151‑1 (MQ=255)        cTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGaa                                                                                                                                          <  1:209982/151‑1 (MQ=255)                 gTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGa                                                                                                                                 >  1:322570/1‑151 (MQ=255)                   tCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAAc                                                                                                                               <  2:283833/151‑1 (MQ=255)                    cATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAAcc                                                                                                                              <  2:422864/151‑1 (MQ=255)                    cATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAAcc                                                                                                                              <  2:207524/151‑1 (MQ=255)                        aCCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATg                                                                                                                          <  1:610669/151‑1 (MQ=255)                               gcTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATgg                                                                                                                   >  1:548054/1‑151 (MQ=255)                                cTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATgg                                                                                                                   >  1:316894/1‑150 (MQ=255)                                   tAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGcttc                                                                                                               <  2:522866/151‑1 (MQ=255)                                         cTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTc                                                                                                           <  1:266639/149‑1 (MQ=255)                                           tCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAg                                                                                                       <  2:156116/151‑1 (MQ=255)                                                 aTAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTcg                                                                                                 >  1:318074/1‑151 (MQ=255)                                                       gCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCaa                                                                                           >  2:367809/1‑151 (MQ=255)                                                             gCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCgg                                                                                       >  1:491320/1‑149 (MQ=255)                                                              cTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCgg                                                                                       >  1:291838/1‑148 (MQ=255)                                                                    cGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAAt                                                                                 <  2:543781/148‑1 (MQ=255)                                                                      ttGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGa                                                                            >  1:579222/1‑151 (MQ=255)                                                                                 gTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGat                                                                  <  1:244161/150‑1 (MQ=255)                                                                                            aGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACgttgtt                                                      >  1:434299/1‑151 (MQ=255)                                                                                              ccACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGttgttg                                                     <  2:613092/150‑1 (MQ=255)                                                                                                         aCCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTca                                         >  1:478421/1‑151 (MQ=255)                                                                                                         aCCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTca                                         >  2:231214/1‑151 (MQ=255)                                                                                                           cAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTcac                                        >  1:440160/1‑150 (MQ=255)                                                                                                           cAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTcac                                        >  1:36794/1‑150 (MQ=255)                                                                                                                 cagTGTTTATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTcaccac                                     <  2:348333/147‑1 (MQ=255)                                                                                                                 cagTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTcaccac                                     <  2:350843/147‑1 (MQ=255)                                                                                                                 cagTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTcaccac                                     <  2:296036/147‑1 (MQ=255)                                                                                                                 cagTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTcaccac                                     <  1:8355/147‑1 (MQ=255)                                                                                                                 cagTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTcaccac                                     <  1:104872/147‑1 (MQ=255)                                                                                                                 cagTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGt                                  <  1:22108/150‑1 (MQ=255)                                                                                                                     gtTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTtgatg                             >  1:460118/1‑151 (MQ=255)                                                                                                                     gtTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTtgatg                             >  2:77293/1‑151 (MQ=255)                                                                                                                                            cTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCAt      <  2:453896/151‑1 (MQ=255)                                                                                                                                              tttGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATag    >  1:272832/1‑151 (MQ=255)                                                                                                                                              tttGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATag    <  1:123935/151‑1 (MQ=255)                                                                                                                                                tGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATagag  <  2:343953/151‑1 (MQ=255)                                                                                                                                                tGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATagag  <  1:406145/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAG  >  NC_000913/2907217‑2907510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑