Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,803	C→T	P520L (CCG→CTG)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,803	0	C	T	100.0%	179.3 / NA	47	P520L (CCG→CTG)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/24);  total (23/24)

CGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGG  >  NC_000913/4182665‑4182941                                                                                                                                           |                                                                                                                                           cGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGTCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGAt                                                                                                                                <  2:395848/151‑1 (MQ=255) cGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGAt                                                                                                                                <  2:474594/151‑1 (MQ=255) cGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGAt                                                                                                                                <  1:386010/151‑1 (MQ=255) cGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGAt                                                                                                                                <  1:636441/151‑1 (MQ=255)   gTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTa                                                                                                                              >  2:473963/1‑151 (MQ=255)      aaaGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGc                                                                                                                           <  1:148981/151‑1 (MQ=255)              tctgtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAAcgt                                                                                                                   <  1:197889/151‑1 (MQ=255)                 gtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAAcgtcgt                                                                                                                >  1:343705/1‑151 (MQ=255)                   ctctGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTAt                                                                                                              <  2:189934/151‑1 (MQ=255)                           gATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCa                                                                                                       >  1:233608/1‑150 (MQ=255)                                ggATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCgg                                                                                                  >  2:579860/1‑150 (MQ=255)                                ggATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCgg                                                                                                  >  1:632102/1‑150 (MQ=255)                                 gATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGc                                                                                                 >  2:229661/1‑150 (MQ=255)                                  aTACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGcc                                                                                                <  1:502806/150‑1 (MQ=255)                                     cccTGATGCCACAGGATATGATCGACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAgg                                                                                            >  2:340493/1‑151 (MQ=255)                                     cccTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAgg                                                                                            >  2:489842/1‑151 (MQ=255)                                     cccTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAgg                                                                                            >  2:359625/1‑151 (MQ=255)                                      ccTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGc                                                                                           <  2:412598/151‑1 (MQ=255)                                            gCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGt                                                                                        >  2:269569/1‑148 (MQ=255)                                                  ggATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt                                                                               >  1:506032/1‑151 (MQ=255)                                                  ggATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt                                                                               >  2:43792/1‑151 (MQ=255)                                                  ggATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt                                                                               >  1:476418/1‑151 (MQ=255)                                                  ggATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt                                                                               >  2:379547/1‑151 (MQ=255)                                                  ggATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt                                                                               >  2:271900/1‑151 (MQ=255)                                                   gATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAcc                                                                                  <  1:607746/147‑1 (MQ=255)                                                   gATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAcc                                                                                  <  2:83661/147‑1 (MQ=255)                                                            aaCGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAg                                                                     <  2:193186/151‑1 (MQ=255)                                                                             cGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGAc                                                    <  2:125916/151‑1 (MQ=255)                                                                                    gtaaaaGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGATCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGAACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTacac                                              >  2:599591/4‑150 (MQ=255)                                                                                    gTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTacac                                              >  2:618228/1‑150 (MQ=255)                                                                                    gTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTacac                                              >  1:476930/1‑150 (MQ=255)                                                                                    gTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTacac                                              >  1:4770/1‑150 (MQ=255)                                                                                     tGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACAcc                                             <  2:475736/150‑1 (MQ=255)                                                                                           agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACt                                        <  2:599017/149‑1 (MQ=255)                                                                                               cttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTa                                   >  2:454846/1‑150 (MQ=255)                                                                                               cttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTa                                   >  2:488119/1‑150 (MQ=255)                                                                                               cttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTAc                                  <  2:557926/151‑1 (MQ=255)                                                                                                  cGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGt                               <  1:434932/151‑1 (MQ=255)                                                                                                           ccagcTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTAt                        <  1:29856/149‑1 (MQ=255)                                                                                                               cTGTCTCAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTcc                    <  2:530799/149‑1 (MQ=255)                                                                                                                  tctcAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCg               <  1:394334/151‑1 (MQ=255)                                                                                                                     cAGTTTATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaaa            >  1:356610/1‑151 (MQ=255)                                                                                                                        tttATGGACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAcc          <  1:8902/150‑1 (MQ=255)                                                                                                                              gACCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg   >  2:84694/1‑151 (MQ=255)                                                                                                                               aCCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAgg  <  1:308543/151‑1 (MQ=255)                                                                                                                               aCCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg   <  2:31365/150‑1 (MQ=255)                                                                                                                               aCCAGAACAACCTGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg   <  2:151361/150‑1 (MQ=255)                                                                                                                                           |                                                                                                                                           CGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGG  >  NC_000913/4182665‑4182941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑