Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,025,007	A→C	S8R (AGC→CGC)	yodD →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,025,007	0	A	C	100.0%	130.2 / NA	37	S8R (AGC→CGC)	yodD	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/13);  total (24/13)

GGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACAGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAA  >  NC_000913/2024873‑2025153                                                                                                                                       |                                                                                                                                                   ggCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACg                                                                                                                                    >  2:611025/1‑151 (MQ=255)      cgcCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAgg                                                                                                                               >  2:403761/1‑151 (MQ=255)                     aGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCgtcgatgtcg                                                                                                                >  2:29837/1‑151 (MQ=255)                              tttCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCctg                                                                                                        >  1:448005/1‑150 (MQ=255)                                tctcGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCctg                                                                                                        >  2:123486/1‑148 (MQ=255)                                     aCGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTggcggc                                                                                                <  1:573075/151‑1 (MQ=255)                                        gTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGAt                                                                                             >  2:454356/1‑151 (MQ=255)                                            ataCTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAAt                                                                                         >  2:88766/1‑151 (MQ=255)                                            ataCTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAAt                                                                                         >  1:303793/1‑151 (MQ=255)                                            ataCTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAAt                                                                                         >  2:368867/1‑151 (MQ=255)                                            ataCTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAAt                                                                                         >  2:333298/1‑151 (MQ=255)                                            ataCTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAAt                                                                                         >  1:529859/1‑151 (MQ=255)                                              aCTTAAAAGATGCCAGGCGGACTTAACGACTGGTGGCAACAACAGAGTAACGGATGCGAGGAAAGATGTTGAAAACCGCAGAAAAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGGTGCGGATGCCCTGCTGGCGGCGATCAATgt                                                                                       >  2:106756/1‑150 (MQ=255)                                                   agagATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTa                                                                                  >  1:63957/1‑151 (MQ=255)                                                       aTGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTagcg                                                                               <  2:563392/150‑1 (MQ=255)                                                                                 gcAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACg                                                    >  2:128135/1‑151 (MQ=255)                                                                                  caacaaCAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGa                                                   >  2:117705/1‑151 (MQ=255)                                                                                       acaGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGAtt                                                 >  1:128756/1‑148 (MQ=255)                                                                                          gagTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGaa                                            >  2:356640/1‑150 (MQ=255)                                                                                          gagTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGaa                                            >  2:209599/1‑150 (MQ=255)                                                                                           agTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAaca                                          >  2:16834/1‑151 (MQ=255)                                                                                            gTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAaca                                          >  1:258961/1‑150 (MQ=255)                                                                                                             aGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACg                        >  1:519065/1‑151 (MQ=255)                                                                                                              gatgatGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGt                       >  1:15208/1‑151 (MQ=255)                                                                                                                  atgaAAACCGCGAAAGAATACCGCTATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCATCCACAACGATTCTCAACACGTTAGCGTCGATGGACGTGAat                   <  2:403874/151‑1 (MQ=255)                                                                                                                     aaaaCCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAatat                 <  2:447996/150‑1 (MQ=255)                                                                                                                     aaaaCCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAatat                 <  1:527646/150‑1 (MQ=255)                                                                                                                         ccGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATAc            <  2:463911/151‑1 (MQ=255)                                                                                                                         ccGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATAc            <  2:378217/151‑1 (MQ=255)                                                                                                                         ccGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATAc            <  1:364112/151‑1 (MQ=255)                                                                                                                         ccGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATAc            <  1:435491/151‑1 (MQ=255)                                                                                                                         ccGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATAc            <  2:187941/151‑1 (MQ=255)                                                                                                                            cAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACAt          <  1:325521/150‑1 (MQ=255)                                                                                                                            cAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACAt          <  2:188365/150‑1 (MQ=255)                                                                                                                            cAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACAt          <  1:102251/150‑1 (MQ=255)                                                                                                                              aaaGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATgg        >  2:142116/1‑150 (MQ=255)                                                                                                                                   gTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGaa  >  1:82067/1‑151 (MQ=255)                                                                                                                                       |                                                                                                                                                   GGCAGCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACAGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAA  >  NC_000913/2024873‑2025153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑