Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913501,5750AC54.3% 33.3 / 43.7 35V555G (GTG→GGG) ybaLinner membrane putative NAD(P)‑binding transporter
Reads supporting (aligned to +/- strand):  ref base A (12/4);  new base C (0/19);  total (12/23)
Fisher's exact test for biased strand distribution p-value = 2.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-05

TTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATT  >  NC_000913/501426‑501723
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ttAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCAGATTTTACATCCGGCAACCACCGTTTACCCCGTcaccac                                                                                                                                                     <  1:789931/151‑1 (MQ=255)
           tcgTTTCGTAGGCCAGAAAAGGCGTTCACGCCGCATCTGGCTTTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTCCATCCGGAAACCCCCGTTTACCCCGTCCCCCCCTCACccgccg                                                                                                                                          <  1:536279/151‑1 (MQ=37)
           tcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccgcc                                                                                                                                           <  1:173858/150‑1 (MQ=255)
                     cgCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATCCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTTACCCGCCGGTGGCGttt                                                                                                                                 >  1:211254/2‑150 (MQ=255)
                           aTAGGGCTTTCCCGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCTTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCACCCCCCGTTTCCCCCGTCACCACCTCCCCCGCCGGTGGCGTTTCcagcag                                                                                                                          <  2:634663/151‑1 (MQ=255)
                                  tttCCCGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCGTCTTATCATCCCTACATATTTTTCATTTTTTACATCCGGCAACCCCCGTTTCCCCCTTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGc                                                                                                                   <  1:749421/150‑1 (MQ=255)
                                  gTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAg                                                                                                                    >  1:386002/1‑150 (MQ=255)
                                      ccgccgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACACCCGGCACCCACCGTTTCCCCCGTCACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATgg                                                                                                               <  2:613848/150‑1 (MQ=255)
                                                       tGGCTCTCGATGCCTGATGCGCCGCTGGCGCGTCTTATCGTGCCTACATTTTTTTCATTTTTTCCATCCGGCAACCACCTTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                                              <  2:409765/151‑1 (MQ=255)
                                                       tGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTCCATCCGGCACCCCCCGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                                              <  2:448934/151‑1 (MQ=39)
                                                            ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc                                                                                          >  2:185273/1‑150 (MQ=255)
                                                                 tGCCTGATGCGCCGCGGGCGCGTTTTATCATGCCTACATATTTTTCATATTTTACACCCGGCACCCACCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCa                                                                                    <  1:509150/151‑1 (MQ=255)
                                                                   acTGATGCGCCGCTGGCGCGTTTTATGATGCCTACATATTTTTCAGTTTTTACATCGGGCAACCACCTTTTCCCCCTTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCAc                                                                                   <  1:404909/149‑1 (MQ=255)
                                                                     tGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                                                                <  2:308551/151‑1 (MQ=255)
                                                                      gATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                                                                >  2:135067/1‑150 (MQ=255)
                                                                       aTGCGACGCTGGCGCGTCTTATCATGCCTACATTTTTCTCATATTTTACATCCGGCAACCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc                                                                               <  1:554287/150‑1 (MQ=255)
                                                                        tGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                                                                >  1:386636/1‑148 (MQ=255)
                                                                                                 ccTACATATTTTTCATATTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATAc                                                    <  1:191428/151‑1 (MQ=255)
                                                                                                         tttttCATATTTTACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTc                                            <  1:66018/151‑1 (MQ=255)
                                                                                                              cATATTTTACATCCGGCACCCTCCGTTTCCCCCGTCCCCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGt                                       <  2:233773/151‑1 (MQ=255)
                                                                                                                 atTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAt                                    >  2:276933/1‑151 (MQ=255)
                                                                                                                  ttttACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATa                                   >  1:444861/1‑151 (MQ=255)
                                                                                                                     tccacccGGCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATg                                <  1:430933/146‑1 (MQ=255)
                                                                                                                     tACATCCGGCAACCACCGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATg                                <  2:863002/151‑1 (MQ=255)
                                                                                                                              caccccccGTTTCCCCCGCCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGc                       <  1:518029/145‑1 (MQ=255)
                                                                                                                                 ccaccGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaat                    >  1:204320/1‑151 (MQ=255)
                                                                                                                                    ccGTTTACCCCGTCACCCCCCCTCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaataat                 <  1:408135/151‑1 (MQ=255)
                                                                                                                                    ccGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATCATGGGCGCGGGCaataat                 >  1:500085/1‑151 (MQ=255)
                                                                                                                                    ccGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaataat                 >  1:811230/1‑151 (MQ=255)
                                                                                                                                    ccGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaataat                 >  1:209594/1‑151 (MQ=255)
                                                                                                                                     cttttCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtc                <  1:678505/149‑1 (MQ=255)
                                                                                                                                     cttttCCCCCGTCACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtc                <  1:202875/149‑1 (MQ=255)
                                                                                                                                     cGTTTCCCCCGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtc                <  2:468021/151‑1 (MQ=255)
                                                                                                                                      ttttCCCCCGTCCCCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAtct               <  2:576406/150‑1 (MQ=255)
                                                                                                                                           ccccGTTACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAata          >  1:206988/1‑151 (MQ=255)
                                                                                                                                              cGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATcc       <  2:329497/151‑1 (MQ=255)
                                                                                                                                                   ccaccTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGAtt  >  2:159352/1‑151 (MQ=255)
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TTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATT  >  NC_000913/501426‑501723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑