Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,815,907	G→A	A178V (GCG→GTG)	gshA ←	glutamate‑cysteine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,815,907	0	G	A	100.0%	142.5 / NA	39	A178V (GCG→GTG)	gshA	glutamate‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/13);  total (26/13)

GTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCGCAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGC  >  NC_000913/2815759‑2816053                                                                                                                                                     |                                                                                                                                                   gTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCcaca                                                                                                                                                  >  2:484282/1‑151 (MQ=255) gTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCcaca                                                                                                                                                  >  2:395191/1‑151 (MQ=255) gTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCcaca                                                                                                                                                  >  2:288359/1‑151 (MQ=255) gTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCcaca                                                                                                                                                  >  2:787496/1‑151 (MQ=255) gTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCcaca                                                                                                                                                  >  1:476480/1‑151 (MQ=255)   aaTACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGATTTCCTTGCAGGAAAGAAGGACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAAGGCGGAAAGAGCCCACAGa                                                                                                                                                >  1:736166/1‑151 (MQ=255)    atacatagCTCACTCGGATTTCTCAAAAGGCCTCGACGTTGGTTTTCCTTGAAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAAAAGATAAGGACTGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATACCCCACAGaa                                                                                                                                               >  1:388275/1‑151 (MQ=255)     tacatacCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGaaa                                                                                                                                              >  1:152048/1‑151 (MQ=255)                  cGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTgg                                                                                                                                  >  2:255378/1‑150 (MQ=255)                     ttttCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCAt                                                                                                                               <  1:164114/150‑1 (MQ=255)                               cGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCgag                                                                                                                    >  2:604098/1‑151 (MQ=255)                                     cGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGata                                                                                                                 <  2:673696/148‑1 (MQ=255)                                       aCGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCAc                                                                                                             <  2:233440/150‑1 (MQ=255)                                          ttGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCa                                                                                                         >  1:315538/1‑151 (MQ=255)                                           tGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCAc                                                                                                        <  1:198406/151‑1 (MQ=255)                                                              gaagaaCAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCa                                                                                     >  2:527210/1‑151 (MQ=255)                                                                         cGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAagag                                                                          <  2:260064/151‑1 (MQ=255)                                                                                 aTGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGt                                                                   <  2:683021/150‑1 (MQ=255)                                                                                    cACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTg                                                               >  2:740088/1‑151 (MQ=255)                                                                                     aCCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGc                                                              <  1:844364/151‑1 (MQ=255)                                                                                      ccAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCa                                                             >  1:650788/1‑151 (MQ=255)                                                                                      ccAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCa                                                             >  1:285405/1‑151 (MQ=255)                                                                                           cAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCg                                                        >  2:441170/1‑151 (MQ=255)                                                                                             gATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGa                                                      >  1:743335/1‑151 (MQ=255)                                                                                             gATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGa                                                      >  1:75906/1‑151 (MQ=255)                                                                                             gATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGa                                                      >  1:191466/1‑151 (MQ=255)                                                                                               tAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGaaa                                                    <  1:456229/151‑1 (MQ=255)                                                                                                  ggAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATgg                                                 >  2:505801/1‑151 (MQ=255)                                                                                                  ggAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATgg                                                 >  2:229158/1‑151 (MQ=255)                                                                                                  ggAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATgg                                                 >  1:330572/1‑151 (MQ=255)                                                                                                  ggAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAG‑AATTGTAGTGCACGCCGGAAATGGt                                                >  1:856829/1‑151 (MQ=255)                                                                                                   gAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATg                                                  <  1:290051/149‑1 (MQ=255)                                                                                                   gAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGt                                                >  1:197248/1‑151 (MQ=255)                                                                                                        aCCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCa                                           >  2:125005/1‑151 (MQ=255)                                                                                                        aCCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCa                                           >  1:677416/1‑151 (MQ=255)                                                                                                         cccAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCAt                                          >  1:626911/1‑151 (MQ=255)                                                                                                                       tAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAg                             >  2:772360/1‑150 (MQ=255)                                                                                                                       tAGTAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGCAg                             >  1:648366/1‑150 (MQ=255)                                                                                                                          tAATTGCGGATAACGCGGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGAt                         <  2:544059/151‑1 (MQ=255)                                                                                                                                       cgcgGAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCtt             >  2:481784/1‑150 (MQ=255)                                                                                                                                           gAAATAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGa        >  2:613286/1‑151 (MQ=255)                                                                                                                                            aaaTAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGAt       <  2:711450/151‑1 (MQ=255)                                                                                                                                            aaaTAGCCCACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGAt       <  1:344035/151‑1 (MQ=255)                                                                                                                                                  cccACAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGc  <  2:845537/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GTAATACATACCGCACTCGGTTTTCTCAAACGGCAGCGACGTTGGTTTTCCTTGCAGGAAAGAAGAACAAATCGCCGGAGATGCACCAAACAGATAAGGAATGACCCAACCGAAACGATAGTAATTGCGGATAACGCGGAAATAGCCCGCAGAAATTTTCTCTTTGGCATCAGCGCCCGAGATATCACCGCACTTCGCTTGCCAGAATGCCATTGGCAAAGAGAAATTGTAGTGCACGCCGGAAATGGTTTGCATCAGCGCGCCGTAGCGATTTTTCAGCCCTTCACGATACAGC  >  NC_000913/2815759‑2816053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑