Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,248,304	C→T	Q112* (CAG→TAG)	lamB →	maltose outer membrane porin (maltoporin)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,248,304	0	C	T	100.0%	136.5 / NA	37	Q112* (CAG→TAG)	lamB	maltose outer membrane porin (maltoporin)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/19);  total (18/19)

TGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACAT  >  NC_000913/4248159‑4248440                                                                                                                                                  |                                                                                                                                         tgAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGt                                                                                                                                     <  2:368552/151‑1 (MQ=255)    aaCTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTaaa                                                                                                                                  >  1:815192/1‑151 (MQ=255)      cTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTaaaaa                                                                                                                                >  2:359745/1‑151 (MQ=255)         aTGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAAc                                                                                                                               >  1:657545/1‑149 (MQ=255)              gAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCg                                                                                                                        >  1:327514/1‑151 (MQ=255)              gAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCg                                                                                                                        >  1:705509/1‑151 (MQ=255)                    aaaTTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATgg                                                                                                                   >  2:625396/1‑150 (MQ=255)                         gggTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCa                                                                                                             >  1:619268/1‑151 (MQ=255)                            tCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGc                                                                                                          <  2:436397/151‑1 (MQ=255)                                   gtgtGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTccacca                                                                                                   >  2:738138/1‑151 (MQ=255)                                    tgtgGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTccacca                                                                                                   <  1:805381/150‑1 (MQ=255)                                      tgGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCt                                                                                                <  2:476495/151‑1 (MQ=255)                                              gggCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGt                                                                                        >  2:361179/1‑151 (MQ=255)                                                    tAAGCGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGAGCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAgcgc                                                                                  <  1:275159/151‑1 (MQ=255)                                                     aaGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCt                                                                                 <  1:241532/151‑1 (MQ=255)                                                       gagCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCtt                                                                                >  2:96360/1‑150 (MQ=255)                                                        agCTTCTATTTCGACACTAACGTGGCCTATTCCGTTGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTc                                                                               <  2:34575/150‑1 (MQ=255)                                                           ttCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTAcc                                                                           <  2:6142/151‑1 (MQ=255)                                                              tATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAAc                                                                        <  1:214872/151‑1 (MQ=255)                                                                     acacTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGa                                                                 >  1:477846/1‑151 (MQ=255)                                                                       acTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACg                                                               <  1:688694/151‑1 (MQ=255)                                                                          aaCGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGtt                                                             >  2:641888/1‑150 (MQ=255)                                                                               ggCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATg                                                       >  2:515018/1‑151 (MQ=255)                                                                                 ccTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGAt                                                     <  1:746375/151‑1 (MQ=255)                                                                                 ccTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGAt                                                     <  2:170669/151‑1 (MQ=255)                                                                                                 acaGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTgg                                     >  1:41748/1‑151 (MQ=255)                                                                                                         aCTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTc                             <  2:537589/151‑1 (MQ=255)                                                                                                         aCTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTc                             <  2:53974/151‑1 (MQ=255)                                                                                                         aCTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTc                             <  2:627678/151‑1 (MQ=255)                                                                                                          cTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATAttt                              >  1:335936/1‑149 (MQ=255)                                                                                                             ggAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGt                         >  2:546157/1‑151 (MQ=255)                                                                                                              gAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGt                         <  1:375475/150‑1 (MQ=255)                                                                                                                     ccGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGc                 <  1:564636/151‑1 (MQ=255)                                                                                                                      cGATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGcc                <  2:717767/151‑1 (MQ=255)                                                                                                                       gATCCGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCg               >  1:783856/1‑151 (MQ=255)                                                                                                                          ccGGCCTTCCGTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTc            <  2:839162/151‑1 (MQ=255)                                                                                                                                    gTGAAGCAAACGTGTAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACAt  >  2:769359/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                         TGAAACTTATGCTGAATTAAAATTGGGTCAGGAAGTGTGGAAAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACAT  >  NC_000913/4248159‑4248440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑