Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,818,103 A→T V133E (GTA→GAA)  chbF ← phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,818,1030AT100.0% 179.3 / NA 48V133E (GTA→GAA) chbFphospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/23);  total (25/23)

GATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGT  >  NC_000913/1817973‑1818248
                                                                                                                                  |                                                                                                                                                 
gATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGa                                                                                                                               >  1:289978/1‑151 (MQ=255)
     gAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGt                                                                                                                          >  1:237884/1‑151 (MQ=255)
     gAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGt                                                                                                                          >  2:506385/1‑151 (MQ=255)
     gAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGt                                                                                                                          >  1:434571/1‑151 (MQ=255)
     aaaTATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATgg                                                                                                                           <  2:528077/149‑1 (MQ=255)
       atatTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTa                                                                                                                         >  2:81842/1‑150 (MQ=255)
       atatTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTa                                                                                                                         >  1:693384/1‑150 (MQ=255)
       atatTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTa                                                                                                                         >  2:699456/1‑150 (MQ=255)
       atatTACACACGCCGATAAAGCGTTAAAATCCGGTAAGAGGATAAACGGATTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTGTTCGATGTCAAAAATCACCGGAATGGTAc                                                                                                                        <  1:528409/151‑1 (MQ=255)
         atTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGc                                                                                                                      <  2:379450/151‑1 (MQ=255)
            acacacGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGa                                                                                                                   >  1:597464/1‑151 (MQ=255)
             cacacGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGAc                                                                                                                  <  1:448709/151‑1 (MQ=255)
                   ccGATAAAGCGTTTAAATCCGGTATGACGGTAAACGGCTTCAGTGAACATTCCCGCAGAGTTAGTAAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTAGATGTCAAGAATCACCGGAATAGGACGCAGACCTTTaa                                                                                                            >  1:222504/1‑151 (MQ=255)
                      aTAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTaaac                                                                                                          <  2:429547/150‑1 (MQ=255)
                            cGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAAccg                                                                                                    <  1:503874/150‑1 (MQ=255)
                                    tCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCg                                                                                           <  2:265583/151‑1 (MQ=255)
                                    tCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCg                                                                                           <  2:666072/151‑1 (MQ=255)
                                       ggTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTg                                                                                        >  1:665431/1‑151 (MQ=255)
                                        gTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTgg                                                                                       >  1:847181/1‑151 (MQ=255)
                                        gTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTgg                                                                                       >  2:531949/1‑151 (MQ=255)
                                             aCGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTc                                                                                   >  2:862369/1‑150 (MQ=255)
                                                        cTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGAt                                                                        <  1:288335/150‑1 (MQ=255)
                                                          tCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATaa                                                                      >  2:441549/1‑150 (MQ=255)
                                                           cAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAAc                                                                     >  1:765065/1‑150 (MQ=255)
                                                             gTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCAt                                                                  >  2:557275/1‑151 (MQ=255)
                                                              tGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAAcc                                                                    <  2:613882/148‑1 (MQ=255)
                                                                aCCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGAc                                                               <  2:56346/151‑1 (MQ=255)
                                                                    ttCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTaa                                                           >  2:165140/1‑151 (MQ=255)
                                                                             ggTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATAc                                                   <  2:680380/150‑1 (MQ=255)
                                                                              gTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACg                                                  <  1:769018/150‑1 (MQ=255)
                                                                                     aaGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATcc                                           <  2:334323/150‑1 (MQ=255)
                                                                                     aaGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATcc                                           <  2:619977/150‑1 (MQ=255)
                                                                                     aaGGTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCa                                          >  2:407979/1‑151 (MQ=255)
                                                                                       gTTAATCACCCACGCATTCGCACAAAGTTCTTCGAAATCTTTTTCGATGTCCAAAAGTACCGCAATGGTACGCAGACCTTTAAACGAACCGCCTGCGCCGTTGGTTTCATGACCAAGACACCCATGAATTAATGGAATACGCTCATCCAGt                                        >  1:823933/1‑151 (MQ=255)
                                                                                        ttAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGtt                                       <  2:748722/151‑1 (MQ=255)
                                                                                        ttAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGtt                                       <  2:714981/151‑1 (MQ=255)
                                                                                          aaTCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCa                                     >  1:386516/1‑151 (MQ=255)
                                                                                               cccATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCAcgcg                                 >  2:556074/1‑150 (MQ=255)
                                                                                                     cATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGTGCCGGTaa                          <  1:166044/151‑1 (MQ=255)
                                                                                                     cATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTaa                          <  2:668941/151‑1 (MQ=255)
                                                                                                          ggACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTgg                      >  1:136072/1‑150 (MQ=255)
                                                                                                                 gTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAAcgcg              >  1:252197/1‑151 (MQ=255)
                                                                                                                          cATCTTTTTCGATGTCAAAAATCCCCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgt     <  2:213342/151‑1 (MQ=255)
                                                                                                                           aTCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgta    >  2:814250/1‑151 (MQ=255)
                                                                                                                            tCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtag   <  1:399994/151‑1 (MQ=255)
                                                                                                                            tCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtag   >  1:479859/1‑151 (MQ=255)
                                                                                                                            tCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtag   <  1:654336/151‑1 (MQ=255)
                                                                                                                             cTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtagt  <  2:515143/151‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                                 
GATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGT  >  NC_000913/1817973‑1818248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑