Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,025,007	A→C	S8R (AGC→CGC)	yodD →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,025,007	0	A	C	100.0%	107.6 / NA	31	S8R (AGC→CGC)	yodD	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/15);  total (16/15)

GCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACAGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTG  >  NC_000913/2024877‑2025151                                                                                                                                   |                                                                                                                                                 gcgcCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAg                                                                                                                              >  1:474934/1‑151 (MQ=255)               cgAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCgtcgatg                                                                                                                 <  2:447004/150‑1 (MQ=255)                       gATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGcc                                                                                                         >  1:713556/1‑150 (MQ=255)                                 aCGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTggcggc                                                                                              <  2:842248/151‑1 (MQ=255)                                  cGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGgcggcg                                                                                             >  2:586138/1‑151 (MQ=255)                                   ggTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGa                                                                                            >  2:806628/1‑151 (MQ=255)                                   ggTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGa                                                                                            >  1:826441/1‑151 (MQ=255)                                    gTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGAt                                                                                           >  2:771841/1‑151 (MQ=255)                                    gTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGAt                                                                                           >  2:108741/1‑151 (MQ=255)                                    gTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGAt                                                                                           >  2:808967/1‑151 (MQ=255)                                      cTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCa                                                                                         >  1:355003/1‑151 (MQ=255)                                        ataCTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAAt                                                                                       >  2:491975/1‑151 (MQ=255)                                        ataCTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAAt                                                                                       >  2:128258/1‑151 (MQ=255)                                             tAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAAt                                                                                  >  2:495022/1‑151 (MQ=255)                                             tAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAAt                                                                                  <  1:348470/151‑1 (MQ=255)                                             tAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAAt                                                                                  <  2:431882/151‑1 (MQ=255)                                                                 tAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATc                                                              <  1:201406/151‑1 (MQ=255)                                                                                 caacaGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGAtt                                               >  2:521150/1‑150 (MQ=255)                                                                                 caacaGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGAtt                                               >  1:444188/1‑150 (MQ=255)                                                                                 caacaGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTc                                              <  1:670785/151‑1 (MQ=255)                                                                                 caacaGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTc                                              <  1:315309/151‑1 (MQ=255)                                                                                      gagTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGaa                                          >  2:106274/1‑150 (MQ=255)                                                                                      gagTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGaa                                          >  2:661820/1‑150 (MQ=255)                                                                                                tGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGt                               <  2:726204/151‑1 (MQ=255)                                                                                                                   aaCCGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAGAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCAt            <  2:305555/151‑1 (MQ=255)                                                                                                                     ccGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATAc          <  2:252442/151‑1 (MQ=255)                                                                                                                     ccGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATAc          <  1:338214/151‑1 (MQ=255)                                                                                                                     ccGCAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATAc          <  2:581468/151‑1 (MQ=255)                                                                                                                        cAAAAGAGTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACAt        <  1:558012/150‑1 (MQ=255)                                                                                                                        cAAAAGAGTAACGCGATGCCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACAt        <  1:168709/150‑1 (MQ=255)                                                                                                                              agTACCGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTg  <  2:174565/150‑1 (MQ=255)                                                                                                                                   |                                                                                                                                                 GCGCCAGGCGCGCGCGAGTGCTGATTTTCTCGACGGTCTATACTTAAGAGATGCCAGGCGGACTTAACGACTGGCGGCAACAACAGAGTAACGGTTGCGAGGAAAGATGATGAAAACCGCAAAAGAGTACAGCGATACCGCAAAACGTGAGGTCAGCGTCGATGTCGATGCCCTGCTGGCGGCGATCAATGAAATTAGCGAAAGCGAAGTTCATCGCAGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTG  >  NC_000913/2024877‑2025151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑