Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,222,5960GA45.1% 113.8 / 59.8 102F13F (TTC→TTTymgDuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (45/11);  new base A (0/46);  total (45/57)
Fisher's exact test for biased strand distribution p-value = 7.74e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

AACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCACC  >  NC_000913/1222449‑1222745
                                                                                                                                                      |                                                                                                                                                     
aaCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGaaa                                                                                                                                                    <  2:1590551/151‑1 (MQ=255)
   gCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaaca                                                                                                                                                 <  2:1354149/151‑1 (MQ=255)
   gCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaac                                                                                                                                                  >  2:634750/1‑150 (MQ=255)
     tGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaacaa                                                                                                                                                >  2:1385125/1‑150 (MQ=255)
     tGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaacaa                                                                                                                                                >  1:121165/1‑150 (MQ=255)
      ggCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaacaaac                                                                                                                                              <  2:1086006/151‑1 (MQ=255)
      ggCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaacaaa                                                                                                                                               <  1:269603/150‑1 (MQ=255)
       gCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaacaaaaa                                                                                                                                             <  2:132298/151‑3 (MQ=255)
       gCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAaacaaaca                                                                                                                                             <  2:109382/151‑1 (MQ=255)
         attacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAgg                                                                                                                                           >  1:886429/1‑151 (MQ=255)
           tacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGc                                                                                                                                          <  1:438530/150‑1 (MQ=255)
            acccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGccc                                                                                                                                        <  1:907159/151‑1 (MQ=255)
                attaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGcc                                                                                                                                     >  1:1513272/1‑150 (MQ=255)
                   accGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGt                                                                                                                                  >  2:1351172/1‑150 (MQ=255)
                       caacatTTTCTGCACCCTGTGCGCTGTCATTTTTTGCCATTTCGTTGGTTTCTGCAAAAGTAATATAGTTTTCTGACGGAAAACCTTTTTTAATATTGTAGTCCTGCCCCCAGGTCATTGGTGAAAAAACAAACAGGCCCGCCAGTAAAGc                                                                                                                             <  1:166208/151‑1 (MQ=255)
                        aacaaTTTCTGCACCCTGTGCGCTGTCAGTTTTTGCCTTTTCGTTGGTTTCTGCGCAAGTAATATAGGTTTCTGCCGAAAAACCTTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCa                                                                                                                            <  1:502401/151‑1 (MQ=255)
                         acaaTTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCa                                                                                                                            >  2:1303807/1‑150 (MQ=255)
                             tttCTGCACCCTGTGCGCTGTCTTTTTTTGCCATTTCGTTGGTTTCTGCGCAATTAATATAGGTTTCTGACGGCAACCCTTTTTAAATATTGTAGTCCTGCACCCAGGTCATGGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAAtt                                                                                                                        <  1:824358/150‑1 (MQ=255)
                                  gCACCCTGTGCGCTGTCATTTTTTGCCATTTCGTTGGTTTCTGCGAAAGTAATATAGTTTTCTGACGGAAAACCTTTTTAAATTTTGTAGCCCTGCCCCCAGTTCTTGGGTGAAAAAAAAAACGGCCCCGCCAGTAAAGCAAAttttt                                                                                                                     <  1:508317/148‑1 (MQ=255)
                                  gCACCCTGGGCGCTGTCATTTTTTCCCTTTTCTTGGGTTTCTGCGCAAGTAATATAGTTTTCTGACGGAAACCTGTTTTTAATATTGTAGCCCTGCCCCCAGGTCTTTGGTGAAAAAACAAACAGGCCCGCCAGTAAAGCAAAtttttt                                                                                                                    <  2:1135684/149‑1 (MQ=255)
                                   cacccTGTGCGCTGTCATTTTTTGCCATTTCGTTGGTTTCTGCGCAAGTAATATAGTTTTCTGACGGCAACCCTTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTtcat                                                                                                                 <  2:1400495/148‑1 (MQ=255)
                                    acccTGTGCGCTGTCAGTTTTTGCCTTTTCTTGGGTTTCTGCGAAATTAATATAGTTTTCTGCCGAAAACCCTTTTTTAATATTGTACCCCTGCCCCCAGTTCTTTGGTGAAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTtcatc                                                                                                                <  2:1181576/149‑1 (MQ=255)
                                    aaCCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTtcatc                                                                                                                >  1:16118/1‑151 (MQ=255)
                                       cTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTtcatcatc                                                                                                             >  2:846255/1‑151 (MQ=255)
                                         gtgCGCTGTCATTTTTTCCCTTTTCGTTGTTTTCTGCGAAAGTAATATGGGTTTCTGACGACAACCTTTTTTTAATATTGTATTCCTGCGCCCAGTTCTTTGGTGCAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTtcatcatc                                                                                                             <  1:1151586/149‑1 (MQ=255)
                                           gcgcTGTCATTTTTTGCTTTTTCTTTGTTTTCTGCACAAGAAATATAGGTTTCTGACGGCAACCTTTTTTTAATTTTGTAGCCCGGCCCCCAGTTTTTGGGGCCAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCAtt                                                                                                          <  1:679156/150‑1 (MQ=255)
                                            cgcTGTCAGTTTTTGCCTTTTCGTTGGTTTCTGCGAAAGTAATATAGTTTTCTGACGGCAACCCTTTTTTAATATTGTAGCCCTGCGCCAAGGTCATTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTcc                                                                                                        <  2:873056/151‑1 (MQ=255)
                                             gcTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCt                                                                                                       >  2:1336190/1‑151 (MQ=255)
                                               tGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCtt                                                                                                      >  2:830564/1‑150 (MQ=255)
                                                gtaattttttGCCTTTTCGTTGGTTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTa                                                                                                     <  2:375316/145‑1 (MQ=255)
                                                gTCAGTTTTTGCTTTTTCGTGGTCTTCTGCGAAAGTAATATAGTTTTCTGACGGCAACCCTTTTTAAATATTGTAGTCCTGCGCCCAGGTTTTGGGTGCAAAAAAAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAt                                                                                                    <  1:634129/151‑1 (MQ=255)
                                                gTCAGTTTTTGCCTTTTCGTTGGCTTCTGCGCAAGTAATATAGTTTTCTGACGGAAACCCTTTTTTAATATTGTAGCCCTGCCCCCAGGTCATTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTa                                                                                                     <  2:686635/150‑1 (MQ=255)
                                                 tCATTTTTTGCCATTTCTTTGGCTTCTGCGAAAGTAATATAGTTTTCTGACGGCAAACCTTTTTTAATATTGTATTCCTGCGCCCAGGTCATTGGTGCAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAt                                                                                                    <  1:794407/150‑1 (MQ=255)
                                                  cAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAttt                                                                                                  >  1:1041157/1‑151 (MQ=255)
                                                   attttttGCCATTTCGTTGGTTTCTGCGCAAGAAATATAGGTTTCTGACGGAAACCCTTTTTAAATATTGTAGTCCTGCCCCCAGTTCTTTGGTGAAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAttt                                                                                                  <  1:299286/148‑1 (MQ=255)
                                                    ttttttCCCTTTTCGTTGGTTTCTGCGCAAGAAATATGGGTTTCGGACGAAAACCCTTTTTTAATTTTGTATTCCGGCCCCCATGTCATTGTTGAGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAttt                                                                                                  <  1:1599489/148‑1 (MQ=255)
                                                     tttttGCTTTTTTGTGGGCTTCTGCGAAAAAAATATAGGTTTCTGACGGAAACCTGTTTTAAATTTTGTAGCCCTGCCCCCAGTTCTTTGGTGAAAAAACAAAAAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCa                                                                                                <  1:1239038/150‑1 (MQ=255)
                                                     tttttGCCTTTTCTTGGGTTTCTGCACAATTAATATAGGTTTCTGCCGGCAACCCTTTTTAAATATTGTACTCCTGCCCCCAGGTCTTTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCa                                                                                                <  2:907416/150‑1 (MQ=255)
                                                      ttttGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAtt                                                                                              >  1:362065/1‑151 (MQ=255)
                                                      ttttGCCATTTCGTGGGTTTCTGCGAAAGAAATATAGTTTTCTGACGGCAACCCTTTTTAAATATTGTAGCCCTGCCCCCAGGTAATTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAt                                                                                               <  2:918369/150‑1 (MQ=255)
                                                       tttGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAttt                                                                                             >  1:1242925/1‑151 (MQ=255)
                                                       tttGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAttt                                                                                             >  1:146663/1‑151 (MQ=255)
                                                        ttGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAtttt                                                                                            >  2:347490/1‑151 (MQ=255)
                                                         tGCCTTTTCGTTGGTTTCTGCGAAAGTAATATAGGTTTCTGACGGCAAACCTTTTTTAATATTGTAGCCCTGCGCCCAGGTCATTGGTGCAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAtttt                                                                                            <  2:137080/150‑1 (MQ=255)
                                                          gCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAc                                                                                          >  2:1150198/1‑151 (MQ=255)
                                                              tttCTTTGGTTTCTGCGAAAGTAATATAGTTTTCTGACGGCAAACTTTTTTAAATATTGTAGTCCTGCCCCAAGTTCTTTGGTGAAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCa                                                                                       <  1:1065326/150‑1 (MQ=255)
                                                              tttCGTTGGTTTCTGCGCAAGTAATATAGGTTTCTGACGGCAACCCTTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCa                                                                                       <  2:530436/150‑1 (MQ=255)
                                                                 cGTTGGTTTCTGCGCAAGTAATATAGTTTTCTGACGGAAAACCTTTTTAAATATTGTAGTCCTGCCCCCAGGTCTTTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                                   <  2:413272/151‑1 (MQ=255)
                                                                 cGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                                   >  1:173953/1‑151 (MQ=255)
                                                                  gTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAtt                                                                                  >  2:343695/1‑151 (MQ=255)
                                                                     ggCTTTTGCGAAATAAATATAGGTTTCTGACGGAAACCTTTTTTAAATTTTGTAGCCCTGCCCCCAGTTCTTTGGTGCAAAAAAAAACAGTCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGc                                                                                <  1:397281/150‑1 (MQ=255)
                                                                     ggCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGc                                                                                >  2:285107/1‑150 (MQ=255)
                                                                        ttCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcacc                                                                             >  1:1181201/1‑150 (MQ=255)
                                                                        ttCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcacc                                                                             >  1:1585745/1‑150 (MQ=255)
                                                                        ttCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcacc                                                                             >  1:698332/1‑150 (MQ=255)
                                                                         tCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcaccac                                                                           >  1:620905/1‑151 (MQ=255)
                                                                            gcgaAAGTAATATAGTTTTCTGACGGAAACCTTTTTTAAATATTGTAGCCCTGCGCCCAGGTCTTGGGAGAAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCg                                                                        <  2:904796/151‑1 (MQ=255)
                                                                             cgcAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGt                                                                       >  2:539207/1‑151 (MQ=255)
                                                                             cgaaaGTAATATAGTTTTCTGACGGAAATCCTTTTTAAATTTTGTAGTCCTGCGCCAAGTTATTTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGt                                                                       <  1:220505/148‑1 (MQ=255)
                                                                              gcAAGTAATATAGGTTTCTGACGGCAACCCTTTTTTAATATTGTAGCCCTGCGCCCAGGTCTTTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTa                                                                      <  1:729876/151‑1 (MQ=255)
                                                                                aattAATATAGTTTTCTGACGACAAACCTTTTTAAATTTTGTAGCCCTGCCCCCAGTTCATGGGTGAAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTAcc                                                                    <  1:561785/148‑1 (MQ=255)
                                                                                 agaaaTATAGTTTTCTGCCGCCAACCCTTTTTAAATTTTGTAGTCCGGCCCCCAGGTCATTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCg                                                                   <  2:362083/148‑1 (MQ=255)
                                                                                 aGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCg                                                                   >  2:433081/1‑151 (MQ=255)
                                                                                  gTAATATAGGTTTCTGACGGCAACCCTTTTTTAATATTGTAGCCCTGCGCCCAGTTCTTTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGc                                                                  <  2:375420/151‑1 (MQ=255)
                                                                                    aaTATAGTTTTCTGACGGCAAACCTTTTTTAATATTGTAGCCCTGCGCCCAGTTCTTTGGGGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCa                                                                <  2:178676/151‑1 (MQ=255)
                                                                                        taGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATc                                                             >  1:196167/1‑150 (MQ=255)
                                                                                         ggttttCTGCCGACAACCTTTTTTTAATTTTTTAGCCCTCCCCCCAGTTCTTTGGTGAAAAAAAAAACGGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCa                                                            <  1:1354522/147‑1 (MQ=255)
                                                                                          gtttccTGCCGGAAAACTTTTTTAAATATTGTGGTCCTGCGCCCAGGTTATGGGTGAAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCGCCCGTACCGCCAATCAc                                                           <  2:884703/145‑1 (MQ=255)
                                                                                          ggTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACg                                                          >  2:880494/1‑151 (MQ=255)
                                                                                               cTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCAcc                                                     >  2:1176505/1‑151 (MQ=255)
                                                                                                tGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCt                                                    >  1:1298615/1‑151 (MQ=255)
                                                                                                tGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAAGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCt                                                    >  1:1469306/1‑151 (MQ=255)
                                                                                                   aggCAAACCTTTTTTTAATATTGTCGCCCTCCCCCCAGTTCTTTGGGGCAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCttt                                                  <  1:640838/150‑1 (MQ=255)
                                                                                                     gCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAAt                                               >  2:180896/1‑151 (MQ=255)
                                                                                                       aaaCCTTTTTTAAATTTGGTGGTCCGCCCCCAGGGTCTTTGGTGAAAAAAAAACCGGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATc                                              <  1:1479846/151‑1 (MQ=255)
                                                                                                       aaaCCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCg                                             >  2:940437/1‑151 (MQ=255)
                                                                                                       aaaCCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCg                                             >  1:737278/1‑151 (MQ=255)
                                                                                                         aCCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGcc                                           >  2:1289480/1‑151 (MQ=255)
                                                                                                          ccctttttaAATTTTGTAGTCCTGCGCCCAGGTCTTTGGTGCAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGcc                                           <  2:614313/147‑1 (MQ=255)
                                                                                                              ttttaAATTTTGTAGCCCTGCCCCCGGGTCATTGGTGCAAAAAAAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCt                                        <  2:1337236/150‑1 (MQ=255)
                                                                                                                 ttAATATTGTAGCCCTGCCCCCAGTTCATTGGTGCAAAAAAAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCg                                     <  2:390166/150‑1 (MQ=255)
                                                                                                                  tAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCg                                     >  2:232065/1‑149 (MQ=255)
                                                                                                                   aaaTTTTGTAGCCCTGCCCCCAGTTCATTGGTAAAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAg                                   <  1:1350921/150‑1 (MQ=255)
                                                                                                                   aaaTATTGTAGCCCTGCCCCCAGTTCTTTGGTGCAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAg                                   <  2:567672/150‑1 (MQ=255)
                                                                                                                        ttGTACCCCTGCCCCCAGTTCATTGGTGAAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCa                               <  2:692899/150‑1 (MQ=255)
                                                                                                                          gTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTg                            >  2:1373312/1‑151 (MQ=255)
                                                                                                                          gTAGTCCTGCGCCCAGGTCATTGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCAtt                             <  2:389642/150‑1 (MQ=255)
                                                                                                                            agcccggcccccAGGTCTTTGGTGAAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGc                           <  2:609086/141‑1 (MQ=255)
                                                                                                                               ccTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGt                       >  2:1002349/1‑151 (MQ=255)
                                                                                                                                    gcCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGa                   >  2:711043/1‑150 (MQ=255)
                                                                                                                                    gcCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGa                   >  1:1377452/1‑150 (MQ=255)
                                                                                                                                    gcCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGa                   >  2:487450/1‑150 (MQ=255)
                                                                                                                                     ccccggttatttGGTGAAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGaa                  <  1:1158802/141‑1 (MQ=255)
                                                                                                                                     cccAGTTCATTGGTGCAAAAAAAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAAc                 <  1:653966/151‑1 (MQ=255)
                                                                                                                                      ccAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACg                >  1:78433/1‑151 (MQ=255)
                                                                                                                                          gttttttGGTGAAAAAACAACCAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCa             <  2:1054655/146‑1 (MQ=255)
                                                                                                                                           gTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGcc              >  2:1236918/1‑149 (MQ=255)
                                                                                                                                            tCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGt           >  1:605174/1‑151 (MQ=255)
                                                                                                                                                     cGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCAcc  >  1:429403/1‑151 (MQ=255)
                                                                                                                                                      gAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCAcc  >  2:1423259/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGACAGCACC  >  NC_000913/1222449‑1222745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: