Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,621,1180AC43.9% 201.1 / 22.1 123V7G (GTG→GGG) eamAcysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base A (44/25);  new base C (0/54);  total (44/79)
Fisher's exact test for biased strand distribution p-value = 1.37e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

CTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAT  >  NC_000913/1620972‑1621265
                                                                                                                                                   |                                                                                                                                                   
cTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACAcccc                                                                                                                                                 >  2:835753/1‑151 (MQ=255)
cTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACAcccc                                                                                                                                                 >  1:592588/1‑151 (MQ=255)
  ttCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCa                                                                                                                                                <  1:660333/150‑1 (MQ=255)
      gTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCttt                                                                                                                                           <  1:1512640/151‑1 (MQ=255)
        cGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTc                                                                                                                                         <  2:1584073/151‑1 (MQ=255)
          tGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTcgc                                                                                                                                       >  2:58813/1‑151 (MQ=255)
             gACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAc                                                                                                                                    <  1:1251630/151‑1 (MQ=255)
               cAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACAt                                                                                                                                  <  2:739589/151‑1 (MQ=255)
                  aaaaGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCt                                                                                                                               >  1:1310922/1‑151 (MQ=255)
                    aaGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTAc                                                                                                                             <  1:791542/151‑1 (MQ=255)
                     aGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTAcc                                                                                                                            <  2:432546/151‑1 (MQ=255)
                       aTAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGc                                                                                                                          >  2:1309326/1‑151 (MQ=255)
                         aGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGcc                                                                                                                         <  2:117576/150‑1 (MQ=255)
                         aGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCct                                                                                                                        <  2:769608/151‑1 (MQ=255)
                          gCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCctc                                                                                                                       <  1:686982/151‑1 (MQ=255)
                           ccGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCctct                                                                                                                      >  2:267991/1‑151 (MQ=255)
                            cGGAAAAGCGACCAGCATAAAGCGCAACCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTg                                                                                                                     <  1:29704/151‑1 (MQ=255)
                              gAAAAGCGACCAGCATAAAGCGCAACCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCAAAAAATTTA‑GCCCCCATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGaa                                                                                                                   <  2:581264/151‑1 (MQ=255)
                              gAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGa                                                                                                                    >  1:863023/1‑150 (MQ=255)
                              gAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGa                                                                                                                    >  2:1251761/1‑150 (MQ=255)
                                 aaGCCACCAGCAAAAAGCGCAACCCGGCCAGCAACAGCGGTGGCATGTTATGAACCCCCACTTTGATGACCACAAAATTTA‑CCCCCCATACGCCCCCTCCCAGTAGCGCAAACCCCCCATCTTTTCCCGACATTCTACCGCCTCTGAAttt                                                                                                                <  1:1445428/151‑1 (MQ=255)
                                   gCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTc                                                                                                               >  1:730608/1‑150 (MQ=255)
                                    cGACCAGCATAAAGCGCAACCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGCCCAAAAAATTTA‑GCCCCCATACGACCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCAt                                                                                                             <  2:741925/151‑1 (MQ=255)
                                     gACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATc                                                                                                            >  2:713763/1‑151 (MQ=255)
                                     gACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATc                                                                                                            >  2:1077762/1‑151 (MQ=255)
                                       ccAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCtt                                                                                                          >  1:1170864/1‑151 (MQ=255)
                                        cAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCttt                                                                                                         >  2:1598914/1‑151 (MQ=255)
                                            aaaaaGCGCAACCCGCCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGCCCACAAAATTTA‑CCCCCCATACGACCACTACCAGTAGCCCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTa                                                                                                     <  1:940192/149‑1 (MQ=255)
                                             tAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTa                                                                                                     >  1:1488363/1‑150 (MQ=255)
                                              aaaGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGCCCACAAAATTTA‑GCCCCCATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAg                                                                                                   <  1:1434833/151‑1 (MQ=255)
                                              aaaGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTa                                                                                                     >  1:359803/1‑149 (MQ=255)
                                              aaaGCCCAAACCAGCCACCAGAAGCGGTGGCTTGTTATAACCCCCCACTTTGATGCCCA‑AAAATTTAGGCCCCCAACCTACCCCTACCATTAGCCCAAACCCCCCATCTTTTCGCGTCTTTCTACCGCCTCTGAATTTCATCTTTTGTAAg                                                                                                   <  1:352754/151‑1 (MQ=255)
                                                      aaCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAAc                                                                                           >  1:1435301/1‑151 (MQ=255)
                                                        ccGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACtt                                                                                         <  1:376807/151‑1 (MQ=255)
                                                        ccGGCCAGAATCAGGGGTGGCATGTTATGAAGCCCCACTTTGATGACCAAAAAATTTA‑GCCCCCATCCGCCCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACtt                                                                                         <  2:177145/151‑1 (MQ=255)
                                                            ccAGCATCAGCGGTGGCATGTTATGAACCCCCACTTTGATAACCAAAAAATTTA‑CCCCCCATACGCCCCCTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCt                                                                                     <  1:62264/151‑1 (MQ=255)
                                                             cAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTg                                                                                    >  1:1175383/1‑151 (MQ=255)
                                                                cATCAGGGGTGGCATTTTAGGACGCCCCACTTTGATGCCCACAAATTTTC‑GCCCCCATACGCCCCCTCCCAATAGCCCCAACCCCCCATCTTTCCGCGACATTCTGCCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAt                                                                                 <  1:314366/151‑1 (MQ=255)
                                                                 aTCAGCGGTGGCATTTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATCCGCCCCCTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAtt                                                                                <  1:482143/151‑1 (MQ=255)
                                                                   cAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAttt                                                                               >  1:1544234/1‑150 (MQ=255)
                                                                    gggggTGGCATGTTATGAAGCCCCATTTTGATGCCCAAAAAATTTA‑CCCCCCATCCGCCCCCTCCCAGTAGCCCCACCCCCCCATTTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTAc                                                                             <  1:910184/148‑1 (MQ=255)
                                                                       ggTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATCCGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACttt                                                                          <  1:1005545/151‑1 (MQ=255)
                                                                       ggTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACttt                                                                          <  2:1552995/151‑1 (MQ=255)
                                                                        gTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACtttt                                                                         >  2:894729/1‑151 (MQ=255)
                                                                          ggCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATCCGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCt                                                                       <  2:1588999/151‑1 (MQ=255)
                                                                              tGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTa                                                                    >  2:127099/1‑150 (MQ=255)
                                                                              tGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTa                                                                    >  2:775723/1‑150 (MQ=255)
                                                                               gTTATGAAGCCCCCCTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTCCCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTaa                                                                   <  2:693993/150‑1 (MQ=255)
                                                                               gTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTaa                                                                   <  2:1415752/150‑1 (MQ=255)
                                                                               gTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGAAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAAc                                                                  >  2:188798/1‑151 (MQ=255)
                                                                                 tATGAAGCCCCCCTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACa                                                                 <  2:1078774/150‑1 (MQ=255)
                                                                                  aTGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGt                                                               >  1:796363/1‑151 (MQ=255)
                                                                                    gAAGCCCCACTTTGATGACCACAAATTTTA‑CCCCCCATACGACCACTACCAGTAGCCCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGtt                                                              <  1:1398042/150‑1 (MQ=255)
                                                                                    gAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGCCCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTg                                                             <  2:107202/151‑1 (MQ=255)
                                                                                    gAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGtt                                                              <  2:1467126/150‑1 (MQ=255)
                                                                                    gAAGCCCCACTTTGATGACCAAAAAATTTA‑GCCCCCATACGCCCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGtt                                                              <  1:197398/150‑1 (MQ=255)
                                                                                     aaGCCCCACTTTGATGCCCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGa                                                            <  2:1278480/151‑1 (MQ=255)
                                                                                     aaGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTg                                                             >  2:50087/1‑150 (MQ=255)
                                                                                      aGCCCCCCTTGGATGACCAAAAAATTTA‑CCCCCCATACGCCCCCTCCCAGTAGCGCAAACCCCCCATCTTTCCGCGACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGAt                                                           <  1:1546465/151‑1 (MQ=255)
                                                                                              tttGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGt                                                   >  1:1006439/1‑151 (MQ=255)
                                                                                               ttGATGACCAAAAAATTTA‑CCCCCCATACGACCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGt                                                   <  2:1291100/150‑1 (MQ=255)
                                                                                                tgatgaCCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCACCACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTc                                                  <  1:609295/150‑1 (MQ=255)
                                                                                                tgatgaCCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCg                                                 >  1:429810/1‑151 (MQ=255)
                                                                                                 gatgaCCACAAAATTTA‑GCCCCCATACGCCCACTACCATTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGc                                                <  1:690307/151‑1 (MQ=255)
                                                                                                  atgaCCAAAAAATTTA‑GCCCCCATACGACCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGcc                                               <  2:732183/151‑1 (MQ=255)
                                                                                                      ccACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGtt                                           >  1:595300/1‑151 (MQ=255)
                                                                                                      ccACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCCGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGtt                                           >  1:856757/1‑151 (MQ=255)
                                                                                                      ccACAAAATTTA‑CCCCCCATACGACCCCTCCAAGTAGCGCCACCCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGtt                                           <  1:632307/151‑1 (MQ=255)
                                                                                                       cacaAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACTCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGtt                                           >  1:1126123/1‑150 (MQ=255)
                                                                                                       caaaaaaTTTA‑GCCCCCATACGCCCACTACCAGTAGCCCCAACCCCCCATTTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTa                                          <  1:101649/148‑1 (MQ=255)
                                                                                                         aaaaaTTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTacg                                        <  2:822201/150‑1 (MQ=255)
                                                                                                             aTTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACgg                                    <  2:74512/151‑1 (MQ=255)
                                                                                                               atA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGc                                   >  1:108368/2‑150 (MQ=255)
                                                                                                                  accccccATACGCCCCCTCCCAGTAGCGCCAACCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCAtt                                <  1:553798/149‑1 (MQ=255)
                                                                                                                   gCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCAtt                                >  1:260818/1‑150 (MQ=255)
                                                                                                                   ccccccATACGACCACCCCCAGTAGCGCCAACCCCCCATTTTTTCGCCACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTa                               <  1:855680/150‑1 (MQ=255)
                                                                                                                    cccccATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTa                               >  2:1362993/1‑150 (MQ=255)
                                                                                                                     ccccATCCGACCCCTCCCAGTAGCCCCAACCCCCCATCTTTTCGCGACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAAt                             <  1:1493590/151‑1 (MQ=255)
                                                                                                                      cccATCCGACCACTCCCAGTAGCCCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATg                            <  2:1466073/151‑1 (MQ=255)
                                                                                                                      cccATACGCCCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATg                            <  2:727665/151‑1 (MQ=255)
                                                                                                                       ccATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgc                           <  1:665575/151‑1 (MQ=255)
                                                                                                                          tACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgcgc                         >  2:1497019/1‑150 (MQ=255)
                                                                                                                             gccccctcccAGTAGCGCAACCCCCCCATCTTTTCGCGACTTTCTGCCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAAt                     <  1:355553/143‑1 (MQ=255)
                                                                                                                               cccctcccAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCTGGTTACGACGGCATTAATGCGCAAATaa                   <  2:964645/145‑1 (MQ=255)
                                                                                                                                 cctcccAGTAGCGCCACCCCCCCATTTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGt                 <  2:103569/147‑1 (MQ=255)
                                                                                                                                    aCCAGTAGCCCCAACCCCCCATCTTTTCGCGACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTc                <  1:57529/149‑1 (MQ=255)
                                                                                                                                          cgcgcCACCCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACt        <  1:560450/150‑1 (MQ=255)
                                                                                                                                          aGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATAc         <  2:329403/150‑1 (MQ=255)
                                                                                                                                           gcgcCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACtt       <  2:1294035/151‑1 (MQ=255)
                                                                                                                                           gcccaaaCACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACtt       <  1:382488/146‑1 (MQ=255)
                                                                                                                                            cgcCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTc      >  2:956654/1‑151 (MQ=255)
                                                                                                                                            cgcCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTc      >  1:720157/1‑151 (MQ=255)
                                                                                                                                             cccAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCg     <  2:223793/150‑1 (MQ=255)
                                                                                                                                               cAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGa   <  1:1478525/151‑1 (MQ=255)
                                                                                                                                               cAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCgg    >  2:1294911/1‑150 (MQ=255)
                                                                                                                                                aaCCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAt  <  1:1105866/151‑1 (MQ=255)
                                                                                                                                                aaCCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAt  <  1:195172/151‑1 (MQ=255)
                                                                                                                                                aaCACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAt  <  2:172504/151‑1 (MQ=255)
                                                                                                                                                aaCACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAt  >  2:1544225/1‑151 (MQ=255)
                                                                                                                                                aaCACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAt  >  2:926037/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTA‑GCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAT  >  NC_000913/1620972‑1621265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑