Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,413	T→A	S359R (AGT→AGA)	thrA →	Bifunctional aspartokinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,413	0	T	A	100.0%	377.4 / NA	96	S359R (AGT→AGA)	thrA	Bifunctional aspartokinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (55/41);  total (55/41)

AATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACC  >  NC_000913/1267‑1560                                                                                                                                                   |                                                                                                                                                    aaTCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCt                                                                                                                                                 >  2:1350929/1‑151 (MQ=255)    cTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCATTCATCTTCCGAATACAGCATCAGATTCTGCg                                                                                                                                              >  2:545041/1‑151 (MQ=255)      gAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGc                                                                                                                                               <  2:928340/148‑1 (MQ=255)       aaTAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTc                                                                                                                                           >  2:1250619/1‑151 (MQ=255)       aaTAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTc                                                                                                                                           >  1:269884/1‑151 (MQ=255)          aaCATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCca                                                                                                                                         <  1:784554/150‑1 (MQ=255)           aCATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCcac                                                                                                                                        >  2:1142238/1‑150 (MQ=255)                   aTGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACt                                                                                                                               <  1:96338/151‑1 (MQ=255)                   aTGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACt                                                                                                                               >  1:744005/1‑151 (MQ=255)                      ttCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCACGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACtgtg                                                                                                                            >  2:1430028/1‑151 (MQ=255)                        cAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACtgtgt                                                                                                                           <  1:794284/150‑1 (MQ=255)                          gCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCg                                                                                                                        >  1:311668/1‑151 (MQ=255)                           cGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCg                                                                                                                        >  2:393593/1‑150 (MQ=255)                            gTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCg                                                                                                                        >  2:1137609/1‑149 (MQ=255)                            gTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCg                                                                                                                        >  1:880512/1‑149 (MQ=255)                            gTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCg                                                                                                                        >  1:1184166/1‑149 (MQ=255)                              ttCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCt                                                                                                                    >  2:17171/1‑151 (MQ=255)                               tCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTg                                                                                                                   >  1:600924/1‑151 (MQ=255)                                  ggTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAAc                                                                                                                <  2:1443562/151‑1 (MQ=255)                                  ggTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAAc                                                                                                                >  1:395372/1‑151 (MQ=255)                                   gTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACg                                                                                                               >  2:211279/1‑151 (MQ=255)                                     ccGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACggg                                                                                                             <  2:343220/151‑1 (MQ=255)                                     ccGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACgg                                                                                                              <  1:818597/150‑1 (MQ=255)                                        gggATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCaa                                                                                                          <  2:13954/151‑1 (MQ=255)                                          gATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATg                                                                                                        >  2:1487840/1‑151 (MQ=255)                                           aTGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc                                                                                                       >  2:723180/1‑151 (MQ=255)                                            tGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc                                                                                                       >  1:63676/1‑150 (MQ=255)                                             gAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc                                                                                                       >  1:1238051/1‑149 (MQ=255)                                             gAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCa                                                                                                      <  2:1242807/150‑1 (MQ=255)                                             gAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAg                                                                                                     <  1:177374/151‑1 (MQ=255)                                              aaaGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg                                                                                                    >  2:1287416/1‑151 (MQ=255)                                               aaGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg                                                                                                    >  2:1278179/1‑150 (MQ=255)                                                 gggATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAag                                                                                                 <  2:807987/151‑1 (MQ=255)                                                    aTGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGACTACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCCATGCAGGAACAGt                                                                                              >  1:534850/1‑151 (MQ=255)                                                    aTGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGt                                                                                              <  2:743984/151‑1 (MQ=255)                                                    aTGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGt                                                                                              >  1:429593/1‑151 (MQ=255)                                                      ggTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTc                                                                                            >  2:302740/1‑151 (MQ=255)                                                      ggTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTc                                                                                            >  2:856280/1‑151 (MQ=255)                                                       gTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCt                                                                                           <  2:425594/151‑1 (MQ=255)                                                         cGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTAc                                                                                         >  2:668586/1‑151 (MQ=255)                                                            cATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTg                                                                                      <  1:1239820/151‑1 (MQ=255)                                                               ggcggcGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGa                                                                                    <  2:39079/150‑1 (MQ=255)                                                                  ggcgCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTg                                                                                >  2:191239/1‑151 (MQ=255)                                                                     gcgcgTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGaaa                                                                             <  1:1065850/151‑1 (MQ=255)                                                                      cgcgTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGaa                                                                              >  2:1612882/1‑149 (MQ=255)                                                                        cgTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAaagaa                                                                          <  1:8034/151‑1 (MQ=255)                                                                            tttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCt                                                                      >  2:991195/1‑151 (MQ=255)                                                                            tttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCt                                                                      >  1:557598/1‑151 (MQ=255)                                                                               gCAGCGATGTCACGCGCTCGTATGGCAGAAGTGAGAATGAAGCAATCATCGTCCGAATACAGAAGAGCAATCAGAGATCCACAAAGCAAAAGATGGCGAAAGGAAGGGGAAAGGGAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTa                                                                    <  1:1175791/150‑1 (MQ=255)                                                                                cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACt                                                                  <  1:1051282/151‑1 (MQ=255)                                                                                 aGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTg                                                                 >  1:1152673/1‑151 (MQ=255)                                                                                   cGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGa                                                               <  1:130446/151‑1 (MQ=255)                                                                                    gATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTgg                                                                >  1:273491/1‑149 (MQ=255)                                                                                            gcgcCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGc                                                      >  2:1388737/1‑151 (MQ=255)                                                                                                ccGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTg                                                  >  2:855654/1‑151 (MQ=255)                                                                                                 cGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGa                                                 <  2:63676/151‑1 (MQ=255)                                                                                                   tATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACg                                               >  1:1217214/1‑151 (MQ=255)                                                                                                    aTTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACgg                                              >  2:252157/1‑151 (MQ=255)                                                                                                    aTTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACg                                               <  1:1260724/150‑1 (MQ=255)                                                                                                      ttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGa                                             >  1:31507/1‑150 (MQ=255)                                                                                                      ttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGa                                             <  1:1519634/150‑1 (MQ=255)                                                                                                        ccGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAAc                                           <  2:142603/150‑1 (MQ=255)                                                                                                        ccGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAAc                                           <  1:478924/150‑1 (MQ=255)                                                                                                        ccGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACg                                          <  2:1183067/151‑1 (MQ=255)                                                                                                        ccGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACg                                          >  1:1485122/1‑151 (MQ=255)                                                                                                         cGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACgg                                         <  2:1055351/151‑1 (MQ=255)                                                                                                         cGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACgg                                         <  1:1266546/151‑1 (MQ=255)                                                                                                             gtgCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTgg                                     >  1:193089/1‑151 (MQ=255)                                                                                                               gCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGcc                                   >  1:775486/1‑151 (MQ=255)                                                                                                                cTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGcc                                   >  1:1338171/1‑150 (MQ=255)                                                                                                                  gATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCAtt                                >  1:993701/1‑151 (MQ=255)                                                                                                                     tACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTAtc                             >  2:860737/1‑151 (MQ=255)                                                                                                                     tACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTAtc                             >  1:1082729/1‑151 (MQ=255)                                                                                                                       cGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTAtctc                           <  2:1531290/151‑1 (MQ=255)                                                                                                                         cAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCgg                         <  1:1142077/151‑1 (MQ=255)                                                                                                                           atcatcTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCggtg                       <  2:964775/151‑1 (MQ=255)                                                                                                                             catcTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCggtgg                      <  1:1322579/150‑1 (MQ=255)                                                                                                                                cTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAgg                  >  2:401064/1‑151 (MQ=255)                                                                                                                                  tCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAgg                  <  1:124117/149‑1 (MQ=255)                                                                                                                                  tCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGt                 <  2:28954/150‑1 (MQ=255)                                                                                                                                  tCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGt                 <  2:667665/150‑1 (MQ=255)                                                                                                                                  tCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTg                >  2:441166/1‑151 (MQ=255)                                                                                                                                   ccGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTg                <  2:269900/150‑1 (MQ=255)                                                                                                                                   ccGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTg                <  1:891184/150‑1 (MQ=255)                                                                                                                                   ccGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTg                >  2:1090370/1‑150 (MQ=255)                                                                                                                                      aaTACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATgg            >  1:638416/1‑151 (MQ=255)                                                                                                                                       aTACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGt           >  1:721018/1‑151 (MQ=255)                                                                                                                                         aCAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTAt         <  2:492836/151‑1 (MQ=255)                                                                                                                                         aCAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTAt         >  2:1361308/1‑151 (MQ=255)                                                                                                                                          cAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATg        <  2:460023/151‑1 (MQ=255)                                                                                                                                           aGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATgc       >  2:143365/1‑151 (MQ=255)                                                                                                                                            gCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATgcg      <  2:1246032/151‑1 (MQ=255)                                                                                                                                            gCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATgcg      >  1:415569/1‑151 (MQ=255)                                                                                                                                             cATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATgcgc     >  1:913290/1‑151 (MQ=255)                                                                                                                                             cATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATgcgc     >  2:1154464/1‑151 (MQ=255)                                                                                                                                                cAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCAcc  <  1:18743/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    AATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACC  >  NC_000913/1267‑1560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑