Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,307,616 C→G D334H (GAT→CAT)  yojI ← microcin J25 efflux ABC transporter permease/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,307,6160CG97.9% 384.4 / 0.4 94D334H (GAT→CAT) yojImicrocin J25 efflux ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base G (44/48);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

TCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGTCGGCAATG  >  NC_000913/2307468‑2307762
                                                                                                                                                    |                                                                                                                                                  
tCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCt                                                                                                                                                  >  2:1291944/1‑151 (MQ=255)
 ccAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCt                                                                                                                                                  <  2:468894/150‑1 (MQ=255)
 ccAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCt                                                                                                                                                  <  1:486818/150‑1 (MQ=255)
     cAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATaa                                                                                                                                             <  2:1320112/151‑1 (MQ=255)
      aaGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATa                                                                                                                                              >  1:903091/1‑149 (MQ=255)
       aGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGc                                                                                                                                           <  1:1054208/151‑1 (MQ=255)
       aGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGc                                                                                                                                           <  1:970304/151‑1 (MQ=255)
         aTTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCaa                                                                                                                                         <  2:978389/151‑1 (MQ=255)
         aTTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCaa                                                                                                                                         <  2:1034601/151‑1 (MQ=255)
         aTTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCa                                                                                                                                          >  2:699480/1‑150 (MQ=255)
            tCGCCGCTTTGTGGCTGGTACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAAc                                                                                                                                       <  2:100686/150‑1 (MQ=255)
             cgccgcTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACg                                                                                                                                      <  2:225699/150‑1 (MQ=255)
             cgccgcTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACg                                                                                                                                      <  1:655524/150‑1 (MQ=255)
             cgccgcTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGt                                                                                                                                     <  2:537964/151‑1 (MQ=255)
                 gcTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACg                                                                                                                                 <  1:147632/151‑1 (MQ=255)
                   tttGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGtt                                                                                                                               >  1:1613589/1‑151 (MQ=255)
                       tgGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGc                                                                                                                           >  2:1472051/1‑151 (MQ=255)
                         gCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGc                                                                                                                           >  1:1388764/1‑149 (MQ=255)
                         gCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGc                                                                                                                           >  1:585772/1‑149 (MQ=255)
                            gATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCt                                                                                                                       <  2:952363/150‑1 (MQ=255)
                            gATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTc                                                                                                                      <  2:892443/151‑1 (MQ=255)
                               aCAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTcc                                                                                                                     >  2:1040003/1‑149 (MQ=255)
                               aCAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCa                                                                                                                    <  2:879548/150‑1 (MQ=255)
                                 aaGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCg                                                                                                                 >  1:1152425/1‑151 (MQ=255)
                                   gCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGtt                                                                                                               <  1:1492923/151‑1 (MQ=255)
                                    cccGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGttt                                                                                                              <  1:767450/151‑1 (MQ=255)
                                     ccGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTg                                                                                                             <  1:1605429/151‑1 (MQ=255)
                                      cGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGc                                                                                                            >  2:276518/1‑151 (MQ=255)
                                       gTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGcc                                                                                                           <  1:668638/151‑1 (MQ=255)
                                          aacaacATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGt                                                                                                        >  2:197470/1‑151 (MQ=255)
                                               caTCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGa                                                                                                   <  1:1348460/151‑1 (MQ=255)
                                                  cGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAAc                                                                                                >  1:715455/1‑151 (MQ=255)
                                                  cGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAAc                                                                                                <  2:234005/151‑1 (MQ=255)
                                                   gCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACg                                                                                               <  1:1350440/151‑1 (MQ=255)
                                                    ccAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGc                                                                                              <  2:1485412/151‑1 (MQ=255)
                                                           cGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCggg                                                                                       <  2:460224/151‑1 (MQ=255)
                                                            gATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGgc                                                                                      <  1:305069/151‑1 (MQ=255)
                                                            gATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGgc                                                                                      >  1:253364/1‑151 (MQ=255)
                                                             aTTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGgcg                                                                                     >  2:755882/1‑151 (MQ=255)
                                                             aTTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGgcg                                                                                     <  2:1256287/151‑1 (MQ=255)
                                                               tttCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGgcgc                                                                                    <  2:914265/150‑1 (MQ=255)
                                                                    gCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACTAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAAc                                                                              <  1:1050264/151‑1 (MQ=255)
                                                                       aCCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAActct                                                                           >  1:236536/1‑151 (MQ=255)
                                                                        ccGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTg                                                                          >  1:623548/1‑151 (MQ=255)
                                                                        ccGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTg                                                                          <  1:803533/151‑1 (MQ=255)
                                                                              ccgccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTg                                                                     >  2:653307/1‑150 (MQ=255)
                                                                              ccgccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGa                                                                    <  1:242394/151‑1 (MQ=255)
                                                                                 ccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGaa                                                                   >  2:936113/1‑149 (MQ=255)
                                                                                  cAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAgg                                                                >  1:1438044/1‑151 (MQ=255)
                                                                                  cAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAgg                                                                >  1:800448/1‑151 (MQ=255)
                                                                                   aaTCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGgc                                                               <  2:251197/151‑1 (MQ=255)
                                                                                        gAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGgcgcg                                                            >  1:430896/1‑149 (MQ=255)
                                                                                         aaaCAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGgcgcgagc                                                         >  1:367487/1‑151 (MQ=255)
                                                                                              gcagcTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGaa                                                    <  2:1051158/151‑1 (MQ=255)
                                                                                               cagcTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAAt                                                   >  2:965518/1‑151 (MQ=255)
                                                                                               cagcTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAAt                                                   >  1:809436/1‑151 (MQ=255)
                                                                                                agcTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAAtt                                                  >  1:684929/1‑151 (MQ=255)
                                                                                                  cTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTg                                                >  2:1097706/1‑151 (MQ=255)
                                                                                                      ccACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTc                                             >  1:1580695/1‑150 (MQ=255)
                                                                                                        aCGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGc                                          >  2:1199163/1‑151 (MQ=255)
                                                                                                         cGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCt                                         >  1:80560/1‑151 (MQ=255)
                                                                                                          gTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCtt                                        >  2:104689/1‑151 (MQ=255)
                                                                                                          gTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCtt                                        >  1:506970/1‑151 (MQ=255)
                                                                                                          gTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCtt                                        <  1:226223/151‑1 (MQ=255)
                                                                                                            ttGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTg                                       <  1:938212/150‑1 (MQ=255)
                                                                                                              gATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGtt                                     >  1:1057294/1‑150 (MQ=255)
                                                                                                              gATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGtt                                     <  2:400477/150‑1 (MQ=255)
                                                                                                                 ggTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTaaa                                  <  2:1591315/150‑1 (MQ=255)
                                                                                                                 ggTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAAc                                 <  1:637373/151‑1 (MQ=255)
                                                                                                                 ggTGAGATTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAAc                                 <  2:41422/151‑1 (MQ=255)
                                                                                                                     agaTTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGcc                              >  2:260234/1‑150 (MQ=255)
                                                                                                                     agaTTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGcc                              >  2:1390600/1‑150 (MQ=255)
                                                                                                                     agaTTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCa                             <  2:715485/151‑1 (MQ=255)
                                                                                                                      gaTTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACCCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCAc                            >  1:289190/1‑151 (MQ=255)
                                                                                                                       aTTAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCAcc                           >  1:296334/1‑151 (MQ=255)
                                                                                                                         tAATCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCt                          <  2:1042334/150‑1 (MQ=255)
                                                                                                                          aaTCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTgc                        <  2:468021/151‑1 (MQ=255)
                                                                                                                          aaTCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTgc                        >  1:1615087/1‑151 (MQ=255)
                                                                                                                          aaTCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTg                         <  2:937382/150‑1 (MQ=255)
                                                                                                                           aTCGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTgcg                       >  1:1473949/1‑151 (MQ=255)
                                                                                                                             cGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGcc                     >  1:287426/1‑151 (MQ=255)
                                                                                                                             cGGACCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGcc                     <  1:982044/151‑1 (MQ=255)
                                                                                                                                aCCAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTc                  <  1:1010873/151‑1 (MQ=255)
                                                                                                                                  cAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTc                  >  1:240025/1‑149 (MQ=255)
                                                                                                                                  cAACGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTc                  >  1:926490/1‑149 (MQ=255)
                                                                                                                                   aaCGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTcagc               <  2:903131/151‑1 (MQ=255)
                                                                                                                                    aCGGAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTcagca              <  2:272353/151‑1 (MQ=255)
                                                                                                                                       gAAAACGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCg           >  1:483779/1‑151 (MQ=255)
                                                                                                                                        aaaaCGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGt          >  1:600906/1‑151 (MQ=255)
                                                                                                                                         aaaCGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGTc         >  2:579653/1‑151 (MQ=255)
                                                                                                                                           aCGCGTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGTCgg       <  1:1098153/151‑1 (MQ=255)
                                                                                                                                            cgcgTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGTCGGc      >  2:470093/1‑151 (MQ=255)
                                                                                                                                              cgTTATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGTCGGCaa    <  2:924512/151‑1 (MQ=255)
                                                                                                                                                 tATGCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGTCGGCAATg  <  2:1560212/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTGCTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGTCAGCAGCGTCGGCAATG  >  NC_000913/2307468‑2307762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: