Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,492,323	IS1 (–) +9 bp	coding (924‑932/1251 nt)	frc ←	formyl‑CoA transferase, NAD(P)‑binding

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	46 (1.050)	36/298	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 2492331	0 (0.000)					coding (924/1251 nt)	frc	formyl‑CoA transferase, NAD(P)‑binding
*	?	NC_000913	= 1979270	NA (NA)	45 (1.030)	31/298	0.2	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	2492323 =	0 (0.000)					coding (932/1251 nt)	frc	formyl‑CoA transferase, NAD(P)‑binding

AGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1979124‑1979270 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCGTTAGG  >  NC_000913/2492323‑2492465                                                                                                                                                                                                                                                                                                    AGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAG                                                                                                                                             >  1:400615/1‑151   ATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAATAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGC                                                                                                                                            <  2:468177/150‑1                        GCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGG                                                                                                                       >  1:582852/1‑150                                       CACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGC                                                                                                        <  2:110846/150‑1                                       CACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGC                                                                                                        <  1:54153/150‑1                                           ACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTAC                                                                                                   <  1:176714/151‑1                                           ACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTAC                                                                                                   <  2:672259/151‑1                                           ACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTAC                                                                                                   <  1:182534/151‑1                                           ACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTAC                                                                                                   <  2:66595/151‑1                                           ACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTAC                                                                                                   <  2:598431/151‑1                                           ACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTAC                                                                                                   <  1:53963/151‑1                                            CCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACT                                                                                                  <  2:454020/151‑1                                                GTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTAT                                                                                              >  1:609599/1‑151                                                 TCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCCCCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTAT                                                                                              <  2:332535/150‑1                                                 TCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTAT                                                                                              <  1:306476/150‑1                                                 TCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTAT                                                                                              <  2:584094/150‑1                                                     TAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGG                                                                                         >  2:455914/1‑151                                                     TAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCC                                                                                           <  1:469390/149‑1                                                     TAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCC                                                                                           <  1:215714/149‑1                                                        CTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGAT                                                                                       <  2:108384/150‑1                                                        CTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGA                                                                                        <  2:508400/149‑1                                                        CTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGA                                                                                        <  2:560567/149‑1                                                                 AGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCC                                                                              <  1:206465/150‑1                                                                   GGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCAT                                                                            <  2:385791/150‑1                                                                   GGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTG                                                                                                 >  1:74986/1‑129                                                                   GGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTG                                                                                                 <  2:74985/129‑1                                                                         CTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGG                                                                       <  1:414861/149‑1                                                                                       AGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTA                                                       >  2:330967/1‑151                                                                                         CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACA                                                     >  2:543446/1‑151                                                                                          CACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAG                                                    <  1:617071/151‑1                                                                                          CACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAG                                                    <  1:43025/151‑1                                                                                            CTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGT                                                  >  2:566793/1‑151                                                                                             TGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTG                                                 <  2:400616/151‑1                                                                                               GAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGT                                                <  2:261338/150‑1                                                                                                    CCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCC                                          <  1:501259/151‑1                                                                                                    CCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCC                                           >  2:422272/1‑150                                                                                                    CCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCC                                           >  2:178334/1‑150                                                                                                             CACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTG                                   >  2:433243/1‑149                                                                                                                 ATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTC                                >  2:379806/1‑148                                                                                                                 ATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTC                                >  1:282894/1‑148                                                                                                                              TCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAA                <  1:112951/151‑1                                                                                                                                  TAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATAT            <  2:184729/151‑1                                                                                                                                       TGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCG       <  1:275849/151‑1                                                                                                                                       TGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCG       <  1:180338/151‑1                                                                                                                                            GCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCGTTAGG  >  2:474620/1‑151                                                                                                                                                                                                                                                                                                    AGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1979124‑1979270 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCGTTAGG  >  NC_000913/2492323‑2492465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.