Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,364	A→T	L193Q (CTG→CAG)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,364	0	A	T	100.0%	105.7 / NA	29	L193Q (CTG→CAG)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/11);  total (18/11)

TTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCAT  >  NC_000913/2907225‑2907506                                                                                                                                            |                                                                                                                                               tttGCCCAGTTCAAAACCAGCAGCTTTAACCGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGaaa                                                                                                                                     <  2:664424/151‑1 (MQ=255)     cccAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGATCCCAGGTTCTGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACt                                                                                                                                  <  1:81578/150‑1 (MQ=255)         gTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTgg                                                                                                                                              <  1:33994/134‑1 (MQ=255)         gTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTgg                                                                                                                                              >  2:33994/1‑134 (MQ=255)         gTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGa                                                                                                                             >  1:18222/1‑151 (MQ=255)         gTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGa                                                                                                                             >  2:449358/1‑151 (MQ=255)         gTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGa                                                                                                                             >  1:434355/1‑151 (MQ=255)           tCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAAc                                                                                                                           <  1:77466/151‑1 (MQ=255)             aTAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAAcc                                                                                                                          >  2:98343/1‑150 (MQ=255)                                               gCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCaa                                                                                       >  1:138822/1‑151 (MQ=255)                                                                 gAACCCAGGTTCGGCGCATAGCCACCTGCGTCACCAACAGCAGTGTTCATGCCTTTCGCGTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCACCCGGCTGAATCATGAAt                                                                      <  2:483191/150‑1 (MQ=255)                                                                  aaCCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAAtt                                                                     >  2:211595/1‑150 (MQ=255)                                                                  aaCCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAAtt                                                                     >  2:235587/1‑150 (MQ=255)                                                                        ggTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGat                                                              >  2:649372/1‑151 (MQ=255)                                                                        ggTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGat                                                              >  2:209169/1‑151 (MQ=255)                                                                              gcgcATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCaag                                                        >  1:578402/1‑150 (MQ=255)                                                                                                cACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTc                                      <  2:293983/151‑1 (MQ=255)                                                                                                   cAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTcac                                    >  2:95711/1‑150 (MQ=255)                                                                                                   cAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTcac                                    >  1:313142/1‑150 (MQ=255)                                                                                                        gcagTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGttt                                                                                                >  2:309517/1‑85 (MQ=255)                                                                                                        gcagTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGttt                                                                                                <  1:309516/85‑1 (MQ=255)                                                                                                        gcagTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGt                              >  2:471865/1‑151 (MQ=255)                                                                                                           gtgtTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTtga                           >  2:80444/1‑151 (MQ=255)                                                                                                             gtTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTtgatg                         >  1:534101/1‑151 (MQ=255)                                                                                                                cATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGatgatg                      <  1:471859/151‑1 (MQ=255)                                                                                                                  tGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGtt                    <  2:6993/151‑1 (MQ=255)                                                                                                                      tttCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTtcatc                <  1:651774/151‑1 (MQ=255)                                                                                                                                   aCTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCa   >  2:88707/1‑151 (MQ=255)                                                                                                                                    cTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCAt  <  2:134492/151‑1 (MQ=255)                                                                                                                                            |                                                                                                                                               TTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCAT  >  NC_000913/2907225‑2907506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑