Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,452	C→G	V164L (GTT→CTT)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,452	0	C	G	100.0%	115.0 / NA	31	V164L (GTT→CTT)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (15/16);  total (15/16)

CACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCA  >  NC_000913/2907320‑2907595                                                                                                                                     |                                                                                                                                                cACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTc                                                                                                                               <  2:293983/151‑1 (MQ=255)    cAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTcac                                                                                                                             >  2:95711/1‑150 (MQ=255)    cAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTcac                                                                                                                             >  1:313142/1‑150 (MQ=255)         gcagTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGt                                                                                                                       >  2:471865/1‑151 (MQ=255)            gtgtTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTtga                                                                                                                    >  2:80444/1‑151 (MQ=255)              gtTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTtgatg                                                                                                                  >  1:534101/1‑151 (MQ=255)                 cATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGatgatg                                                                                                               <  1:471859/151‑1 (MQ=255)                   tGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGtt                                                                                                             <  2:6993/151‑1 (MQ=255)                       tttCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTtcatc                                                                                                         <  1:651774/151‑1 (MQ=255)                                    aCTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCa                                                                                            >  2:88707/1‑151 (MQ=255)                                     cTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCAt                                                                                           <  2:134492/151‑1 (MQ=255)                                           cctggTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGt                                                                                      <  1:40988/147‑1 (MQ=255)                                                tGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTg                                                                                 <  2:455096/150‑1 (MQ=255)                                                         aCTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTAc                                                                       <  2:490153/151‑1 (MQ=255)                                                          cTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTAc                                                                       >  2:604503/1‑150 (MQ=255)                                                          cTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTAc                                                                       >  1:372847/1‑150 (MQ=255)                                                               gAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttca                                                                 >  2:631535/1‑151 (MQ=255)                                                                    cATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagttca                                                            >  1:89639/1‑151 (MQ=255)                                                                        cGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGa                                                        <  2:611178/151‑1 (MQ=255)                                                                                   tttCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTAc                                             <  2:434359/151‑1 (MQ=255)                                                                                             ttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATAc                                   <  1:95713/151‑1 (MQ=255)                                                                                               cgcgCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCt                                 <  2:3323/151‑1 (MQ=255)                                                                                                     aaCCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTagca                           >  1:205690/1‑151 (MQ=255)                                                                                                     aaCCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTagca                           <  1:504368/151‑1 (MQ=255)                                                                                                               aTCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGcagcag                 <  1:209171/151‑1 (MQ=255)                                                                                                                       ttCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCg         >  2:449269/1‑151 (MQ=255)                                                                                                                         ccTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGtt       >  1:192926/1‑151 (MQ=255)                                                                                                                         ccTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGtt       <  1:180353/151‑1 (MQ=255)                                                                                                                              atatCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCa  >  1:674500/1‑151 (MQ=255)                                                                                                                               tatCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCt                                 >  2:393891/1‑119 (MQ=255)                                                                                                                               tatCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCt                                 <  1:393890/119‑1 (MQ=255)                                                                                                                                     |                                                                                                                                                CACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCA  >  NC_000913/2907320‑2907595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑