Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,060,146	C→A	Q132K (CAG→AAG)	argP →	transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,060,146	0	C	A	100.0%	79.9 / NA	23	Q132K (CAG→AAG)	argP	transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/13);  total (10/13)

ATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGCCGAAAAATATTTCC  >  NC_000913/3060000‑3060284                                                                                                                                                   |                                                                                                                                           aTGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAgg                                                                                                                                         <  1:318509/150‑1 (MQ=255)    aaCAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAAc                                                                                                                                      <  1:291275/150‑1 (MQ=255)      cAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTc                                                                                                                                   <  2:479824/151‑1 (MQ=255)        aaCCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGt                                                                                                                                    >  1:470083/1‑148 (MQ=255)           cGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTgcgc                                                                                                                              <  2:574368/151‑1 (MQ=255)                   cTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGa                                                                                                                      <  2:542739/151‑1 (MQ=255)                        ctgctgctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAgggg                                                                                                                 >  1:504221/1‑151 (MQ=255)                           ctgctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTc                                                                                                               <  2:113410/150‑1 (MQ=255)                              ctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGgcg                                                                                                           <  2:341968/151‑1 (MQ=255)                                     tGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAg                                                                                                    <  2:444967/151‑1 (MQ=255)                                       gCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAAGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGt                                                                                                   <  2:247636/150‑1 (MQ=255)                                           tCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTc                                                                                               <  2:103308/150‑1 (MQ=255)                                               cGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACAt                                                                                          >  1:384953/1‑151 (MQ=255)                                                        tCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGgc                                                                                     <  1:515171/147‑1 (MQ=255)                                                                  tGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGTACGTCTGCGCCGCGGCGAAGGGGTCGGCGCGGTGCGGATTCAACATCAGGCGCTGCGGAGttgt                                                                        >  1:345243/1‑150 (MQ=255)                                                                         ttCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGa                                                                >  2:196831/1‑151 (MQ=255)                                                                              gCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATaaa                                                            >  2:87438/1‑150 (MQ=255)                                                                                          gtgtTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCg                                               >  1:506883/1‑151 (MQ=255)                                                                                          gtgtTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCg                                               >  1:185153/1‑151 (MQ=255)                                                                                                gCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTAt                                          <  2:191727/150‑1 (MQ=255)                                                                                                             tCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTc                            <  2:508157/151‑1 (MQ=255)                                                                                                                         tGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTg                 >  1:688486/1‑150 (MQ=255)                                                                                                                                         aaCCCGCACTAAGGAACGTCTGCGCCGCGGCGACGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGCCGAAAAATATTTcc  >  1:523526/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                           ATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGCCGAAAAATATTTCC  >  NC_000913/3060000‑3060284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑