Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	450,073	C→T	W190* (TGG→TAG)	cyoB ←	cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	450,073	0	C	T	100.0%	194.4 / NA	51	W190* (TGG→TAG)	cyoB	cytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/23);  total (28/23)

AATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTAAGT  >  NC_000913/449925‑450214                                                                                                                                                     |                                                                                                                                              aatCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAg                                                                                                                                             <  2:32693/151‑1 (MQ=255)  atCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGt                                                                                                                                            >  2:647457/1‑151 (MQ=255)       tACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGATTCCATATCTAGTAAt                                                                                                                                         <  1:436244/149‑1 (MQ=255)       tACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATc                                                                                                                                        <  1:517204/150‑1 (MQ=255)           ttCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGaca                                                                                                                                    >  1:622833/1‑150 (MQ=255)                     gTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCgg                                                                                                                         >  1:567133/1‑151 (MQ=255)                     gTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCgg                                                                                                                         >  2:305392/1‑151 (MQ=255)                        aTGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGAc                                                                                                                       <  2:181991/150‑1 (MQ=255)                        aTGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGAc                                                                                                                       <  1:41418/150‑1 (MQ=255)                        aTGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGAc                                                                                                                       <  1:390404/150‑1 (MQ=255)                        aTGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGAc                                                                                                                       <  2:508289/150‑1 (MQ=255)                          gCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGa                                                                                                                        >  2:177430/1‑147 (MQ=255)                                gTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTActct                                                                                                               <  1:472890/150‑1 (MQ=255)                                 tAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTAt                                                                                                             <  2:319937/151‑1 (MQ=255)                                 tAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTAt                                                                                                             <  2:260915/151‑1 (MQ=255)                                    aTACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTc                                                                                                           >  1:469572/1‑150 (MQ=255)                                    aTACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTc                                                                                                           >  2:458017/1‑150 (MQ=255)                                        tGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGAt                                                                                                      <  1:375193/151‑1 (MQ=255)                                           cATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGc                                                                                                   <  1:390482/151‑1 (MQ=255)                                                       ggTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgc                                                                                        >  2:652019/1‑150 (MQ=255)                                                        gTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgcc                                                                                       <  2:263983/150‑1 (MQ=255)                                                           aTGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgccagcc                                                                                   <  2:490491/151‑1 (MQ=255)                                                            tGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGgccagcca                                                                                  >  1:393289/1‑151 (MQ=255)                                                                 ggTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCgg                                                                             >  2:588503/1‑151 (MQ=255)                                                                  gTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGt                                                                            >  1:540915/1‑151 (MQ=255)                                                                   tGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTc                                                                           <  2:469578/151‑1 (MQ=255)                                                                         cATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCa                                                                     >  2:498345/1‑151 (MQ=255)                                                                         cATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCa                                                                     >  2:584011/1‑151 (MQ=255)                                                                         cATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCa                                                                     >  1:404334/1‑151 (MQ=255)                                                                                 gAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGc                                                              >  1:1455/1‑150 (MQ=255)                                                                                           gaagaaGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCgaga                                                   >  2:465375/1‑151 (MQ=255)                                                                                                 gTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACg                                             >  2:52443/1‑151 (MQ=255)                                                                                                 gTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACg                                             >  2:548851/1‑151 (MQ=255)                                                                                                      tACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTaa                                         >  2:470554/1‑150 (MQ=255)                                                                                                       aCCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAAcc                                       <  1:30119/151‑1 (MQ=255)                                                                                                            tAAGCGTCGTACCTATACCGGATAGCTGGATACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAAt                                  <  1:146157/151‑1 (MQ=255)                                                                                                                     tACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaac                         <  2:153834/151‑1 (MQ=255)                                                                                                                     tACCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaa                          >  2:415723/1‑150 (MQ=255)                                                                                                                      aCCTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaaca                        >  1:97740/1‑151 (MQ=255)                                                                                                                       ccTATACCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACcaacaa                       <  2:297813/151‑1 (MQ=255)                                                                                                                          ataCCGGATAGCTGGAGACTCTATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACgg                    >  1:671043/1‑151 (MQ=255)                                                                                                                          ataCCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACgg                    >  2:591310/1‑151 (MQ=255)                                                                                                                          ataCCGGATAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACgg                    >  2:516594/1‑151 (MQ=255)                                                                                                                          ataCCGGATAGCTGGAGACCCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACgg                    >  2:682364/1‑151 (MQ=255)                                                                                                                                 aTAGCTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAAcc              <  1:588495/150‑1 (MQ=255)                                                                                                                                     cTGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAg         <  2:378248/151‑1 (MQ=255)                                                                                                                                     cTGGAGACTCCAGATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAg         <  1:64840/151‑1 (MQ=255)                                                                                                                                      tGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAg         >  1:684450/1‑150 (MQ=255)                                                                                                                                      tGGAGACTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGc        <  1:511524/151‑1 (MQ=255)                                                                                                                                            cTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTAAgt  >  1:622579/1‑151 (MQ=255)                                                                                                                                            cTCCATATCTAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTAAgt  >  2:65801/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                              AATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATCCAGTAATCGACACCGACTCCCGGACTGTACTCTATTCCCGATAGCGGTGGATAGGCCAGCCAGCCGGTCTGCGCAAATTCGCCCACGCCGAGAGAAACGTTAACCAGAATCACACCAACAACGGTAAACCAGAAGCTTAAGT  >  NC_000913/449925‑450214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑