Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,282	0	T	G	52.4%	13.9 / 22.3	21	V526G (GTT→GGT)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base G (11/0);  total (16/5)
Fisher's exact test for biased strand distribution p-value = 1.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-04

TGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAAC  >  NC_000913/354136‑354421                                                                                                                                                   |                                                                                                                                            tGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGtgggtggg                                                                                                                                         >  1:545919/1‑151 (MQ=255)          cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCTGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                 <  2:209279/150‑1 (MQ=255)                                  cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGGAgggggcggtg                                                                                                        >  1:117016/1‑151 (MQ=255)                                  cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAggtggcgggg                                                                                                        >  2:516484/1‑149 (MQ=255)                                  cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGGAAGGGCAgggggcgggg                                                                                                        >  1:181840/1‑149 (MQ=255)                                                         aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAagagag                                                                                 >  2:150941/1‑151 (MQ=255)                                                             aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                                                                             <  2:326861/151‑1 (MQ=255)                                                                        ccGGGCAGCGGGTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGACGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                   <  1:310103/150‑1 (MQ=255)                                                                           ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                               >  2:214162/1‑151 (MQ=255)                                                                                cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                          <  2:90271/151‑1 (MQ=255)                                                                                     ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                     >  1:261540/1‑151 (MQ=255)                                                                                                agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAaga                                          >  2:211448/1‑151 (MQ=255)                                                                                                agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTCGGGTGAAAGAAGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTTATTCCGGAAGAGAGGGGCAATCTGGAAGAACGTGATGGGGCGCACTCGCAaga                                          >  1:76830/1‑151 (MQ=255)                                                                                                    tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                      >  2:86526/1‑151 (MQ=255)                                                                                                     gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAgg                                     >  1:184200/1‑151 (MQ=255)                                                                                                          gagTATCTCCAGCCATCTGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                >  2:259521/1‑151 (MQ=255)                                                                                                          gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGGAAGATGCGCTGCAAGGGCAGGGGGCGGGGGCGTTTGGCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCCCTCGCAAGAGAAGGCGAtt                                >  1:351599/1‑151 (MQ=255)                                                                                                          gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                >  2:231085/1‑151 (MQ=255)                                                                                                          gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGAAAAGGCAGGTGGCGGTGGCGTTTTTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGGtt                                >  2:220856/1‑151 (MQ=255)                                                                                                                  ccAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGCGCCCTCGCCAGAGAAGGCGGTTATGgcg                          >  1:100210/1‑149 (MQ=255)                                                                                                                        aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGACCTGAAAGGGCAGGTGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGACTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGAGACTATGGCGCtggtggg                  >  1:337595/1‑150 (MQ=255)                                                                                                                                          tGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAAc  <  2:82180/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                            TGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAAC  >  NC_000913/354136‑354421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑