| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 987,494 | 0 | T | G | 58.3% | 4.1 / 12.2 | 12 | intergenic (‑512/+91) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (7/0); total (8/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-02 | |||||||||||
ACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTT > NC_000913/987354‑987639 | aCATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAg < 2:142165/150‑1 (MQ=255) cATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGaaaaaaaa > 1:392999/1‑146 (MQ=255) aaaaaaTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAAt < 2:178925/134‑1 (MQ=255) cAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAATAAAATTATTTTTTTTAAAAAAAAATAAATTGATAGAAAAGAAAAAAAAAAAGACAGAGGgcg > 1:8727/1‑148 (MQ=255) gAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGGTAAAAGAATTTTTTACTTTACAAAAAAATAAATTGACAGAAAAAAAAAAAAAAAAACCAGCGGGCGCAGGGTCtt > 1:226952/1‑151 (MQ=255) gAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAATTGGTTTACTTAAAAAAAAAATAACTTTACAGGAAAAAAAAAAAAAAAAGCAGCGGGGGCGGGCTCtt > 1:288870/1‑151 (MQ=255) gAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAATTGGTTGTTTTAACCAAAAAATAAATTTATCGAAAAAAAAAAAAAAAAGACAGCCGGGGGTGGgtc > 1:110550/1‑149 (MQ=255) aaTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCg < 1:25173/151‑1 (MQ=255) aTGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAAATTTTTTTCTTAACAAACAAAAAAAATTACAGAAAAAAAAAAAAAAAAACCAACGGGGGCCGGCTCTTTTAGAAACCAAATTAAAAAAAGGCGTTACGCggg > 1:330802/1‑150 (MQ=255) gTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAATAAAATGTTTGTCTTTAAAAAAAAATAAATTTTCAAAAAAAAAAAAAAAAAAAACAACGGGCGCCGGCTCCTGAAAAAACCAAATTAAAAACTGGCGTTTcgccgggg > 2:56852/1‑146 (MQ=255) ccGTAACAGGATGATCGTTATGGCGGGGGTGTAAAAAAATTTCTTTACTTTTCAAACAAATAAATTTAAACAAAAGATAAAAAAAAAAGCAGCGGGGGCTGGGTCTTGAAAAAGGTAAATTAAAAAATGGCGGTATGCGCAGGACGTgggg > 2:230471/1‑150 (MQ=255) cGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGttt < 1:179562/151‑1 (MQ=255) | ACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTT > NC_000913/987354‑987639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |