Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,337,7230AC57.1% 17.5 / 32.8 29G566G (GGT→GGG) gyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base A (9/3);  new base C (0/16);  total (9/20)
Fisher's exact test for biased strand distribution p-value = 3.19e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-05

CCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTG  >  NC_000913/2337574‑2337858
                                                                                                                                                     |                                                                                                                                       
ccGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTa                                                                                                                                         >  2:327803/1‑150 (MQ=255)
 cgcgcgcgCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTAcc                                                                                                                                       <  1:252507/151‑1 (MQ=255)
       cgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTcc                                                                                                                                  >  1:325986/1‑150 (MQ=255)
                      ccGGAAACTGATAAACTTTCATCGAATAGACGCGCCCCCGGCTGGAGAAGCACAAAATATGGTCGTGAGTTTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTgcgc                                                                                                                  <  2:532837/151‑1 (MQ=255)
                                  aaaCTTTCATCGAATAGACGCGCCCACGGCTGGAGAAGCACAGAATAGGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGa                                                                                                      <  2:346651/151‑1 (MQ=255)
                                                     ccgccccgggtgggAGAAGCAAAAAATATGGTCGTGAGTGTTCCCCACCAGCAGTCGGTCGAAAAAGTCTTCTTCTTAAATGCGTGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACg                                                                                   <  1:67466/139‑1 (MQ=255)
                                                           ccggcggGAGAAGCACAGAATATGGTCGTGAGTTTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATGCGTGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGccc                                                                             <  1:152374/150‑1 (MQ=255)
                                                                  gagaAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgag                                                                      >  2:393559/1‑151 (MQ=255)
                                                                   agaAGCACAGAATTTGGTCGTGAGTGTTCCCCCCCAGCAGTCGGTCGATAAGGTCTTCTTCTTTAATGCGTGCGGCAGATTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgag                                                                      <  1:361478/150‑1 (MQ=255)
                                                                   agaAGCACAGAATATGGTCGTGAGTGTTCCCCCCCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgag                                                                      <  1:456155/150‑1 (MQ=255)
                                                                     aaGCACAGAATATGGTCGGGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgagag                                                                    <  2:447854/150‑1 (MQ=255)
                                                                        cacaAAATATGCTGGTGTTGGTTCGCCACCAGCAGTCGGTAGATAATGTCTGCTTCGTTAAGGCTTGGGGAAGATTTCCCTCCCCCGCCACGACGCTGCGCTGCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGagagagag                                                                <  1:102243/151‑3 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa                                                             >  2:131066/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa                                                             >  1:38255/1‑150 (MQ=255)
                                                                            aaaTATGTTCGTGAGTGTTCGCCCCCAGCAGTCGTTCGATAAAGTCTTCTTTTTTAATACGTGCGGCAGATTTTCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCAc                                                            <  2:158201/150‑1 (MQ=255)
                                                                              atatGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGa                                                          >  1:326198/1‑151 (MQ=255)
                                                                                ttggtggGGAGTGTCCCCCCCCAGCAGCCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGAcc                                                        <  2:474187/150‑1 (MQ=255)
                                                                                   gTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACcaca                                                     >  1:268345/1‑151 (MQ=255)
                                                                                              tCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTTTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGt                                          <  2:313280/151‑1 (MQ=255)
                                                                                                  caccaGCAGTCGGTCGAAAAAGTCTCCCTCTTTAATACGTGGGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATc                                      <  1:110800/151‑1 (MQ=255)
                                                                                                                    aaaGTCTTCTTTTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATg                    <  2:105020/151‑1 (MQ=255)
                                                                                                                       gTCTTCTTCTTTAATACGTGCGGTAGATTTAACTTTTCAGCCACGACGCTGCGCATCGTATTCAGAAAGCGGCTGATAATTAAAGTAGCCCTGGTGAGAGCGCGTCACGACCACATCTCCCTGGGCGATCAGATCTTCCAGGTCGATGt                   >  1:337272/1‑149 (MQ=255)
                                                                                                                       gTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGt                   >  1:453177/1‑149 (MQ=255)
                                                                                                                       gTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGt                   >  2:231954/1‑149 (MQ=255)
                                                                                                                           ttttttttAATGCGTGGGGAAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTgcgc             <  2:449406/146‑1 (MQ=255)
                                                                                                                           tttttttaaaTACGTGCGGAAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTgcgc             <  1:131063/143‑1 (MQ=255)
                                                                                                                             ttttttAATCCGGGCGGAAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTg           <  1:327799/148‑1 (MQ=255)
                                                                                                                                tttAATCCGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTg        <  2:532717/151‑1 (MQ=255)
                                                                                                                                tttAATACGGGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTg        <  2:475842/151‑1 (MQ=255)
                                                                                                                                      ccGTGCGGAAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTg  <  1:351316/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                       
CCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTG  >  NC_000913/2337574‑2337858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑