| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,578,287 | 0 | T | C | 50.0% | 25.8 / 12.7 | 38 | E147G (GAA→GGA) | gntR | d‑gluconate inducible gluconate regulon transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (14/5); new base C (0/19); total (14/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGATGATAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAG > NC_000913/3578137‑3578435 | ttGATGATAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGtt < 2:40529/151‑1 (MQ=255) tAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTccacca > 2:43722/1‑150 (MQ=255) tcgtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATa > 1:82064/1‑150 (MQ=255) tcgtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATa > 1:478143/1‑150 (MQ=255) gaCGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCa > 2:166902/1‑150 (MQ=255) catgggggCGAGCCCCGGGAGAAATAAGGGCAGTGGTCTTCTGGCGTGCTTCTTAAAATTTAAAAAAC‑CCGACGG‑CGATAACAAGGCAGGGCGCCTTGCTTTCCATCAGTCCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAgg < 1:113451/144‑1 (MQ=255) cagtgtgGCGATGCCCGCGACAATTAGGGGCGTTGCTCATCTGGCGTGCTGCTTCAAATTTATCAAAC‑CCGCCGG‑CGATATCAAGGCATGCCGCTTTCCTTTCCATCAGCCCCCCCACGGG‑AATACCCGCCACTTCAATCATCTTTAAgg < 1:376287/147‑1 (MQ=255) cagtggggCGATGCCCGCGAGAAATAAGGGGAGTGGTCATCGGGCGTGCTGCTACAAAGTTATAAAAC‑CCGACGG‑AAATATCAAGGCATGGCGACTTGCTGTCCATCAGTCCCACCCCGGG‑AATCCCCGCCACTTCAATCATCTTTAAgg < 2:15547/144‑1 (MQ=255) cAATGTGGCGATCCCCGCGAGAAATAATGGCAGTGGTCATCTGGCGTGCTGCTTAAAAGTTATAAAAC‑CCGACGG‑CAATATCAAGGCATGGCGACTTGCTGTCCATCA‑TTCCCCCCCGGGAAATACCCGCCACTTCAATCATCTTTAAgg < 2:248339/150‑1 (MQ=255) aaaTGGGGCAACGCCCGGAAGAAAAAATGGCGGTGGTCATGTGGCTTGCTGCTTAAAATTTAACAAAC‑CCGACGG‑CGATATAAGGGCATGGCTACTTGCTGTCCACCAGTTCCCCCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAgg < 1:473266/149‑1 (MQ=255) gcgaccccggggAGCAATAATGGCAGTGTCCATCGGGGGTGCTGCTACAAAGTTA‑CAAAA‑CCCACGGCCGATAACAAGGCTTGGCGTTTGGCTGTCAATCAGTCCCCCCAGGGA‑AACCCCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 1:260138/139‑1 (MQ=255) ccGCGAAAAATAAGGGCAGTGGTCCTCTGGGGTGCTCCTTAAAATTAAAAAAAC‑CCGCCGG‑CAATACCAGGGCATGGCGTCTTGCTGCCCATCATTCCCCCCCCGGA‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTggg < 1:508086/151‑1 (MQ=255) cgAGAAATAATGGCGGGGGTCTTCGGGCGTGCTGCTTAAAAGTTATAAAAC‑CCGAGGG‑GGATATCAAGGCATGGCGCTTTGCTGTCCATGGGTCCCCCCCGGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTa < 1:403496/150‑1 (MQ=255) agaaataatGGCAGTGTTCATCGGGGGTGCTGCTTCAAAGTTAAAAAAC‑CCGACGG‑CGATATAAGGGCATGGCGTCTTGCTGCCCATCAGTCCCACCACGGA‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTggg < 2:507329/143‑1 (MQ=255) aaaaaaaagggCGGTGGTCATCTGGCGTGCTGC‑TAAAAATTAACAAAACCCGAGGG‑CGATACAAGGGCAGGGCGATTTGCTCTCCACCAGTCCCACCGGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACg < 1:372050/141‑1 (MQ=255) aGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAC‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTCCCCCCCCGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACg < 2:236806/150‑1 (MQ=255) aGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTggg > 1:507319/1‑146 (MQ=255) gCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGtt > 2:104243/1‑151 (MQ=255) gTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCa > 2:129994/1‑151 (MQ=255) gTGGTCATCGGGCGTGCTGCTTAAAAGTTAAAAAAC‑CCGCCGG‑CAATATCAGGGCAGGGCGCCTTGCTGTCCATCAGTCCCCCCGGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATc < 1:82584/150‑1 (MQ=255) tggtactCTGGCGTCCGTCTTCAAAGTTATCAACC‑CCGCCGC‑CATTATCAGGGCGTGGCGACTTTTTCTCTAACATTTCCACCGCGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATc < 1:466416/143‑1 (MQ=255) ggTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAgg > 2:269755/1‑151 (MQ=255) tgctgcTTAAAAGTTAAAAAAC‑CCGACGG‑CGATATAAAGGCAGGGCTATTTGCTGCCCATCATTCCCCCCCCGGA‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTc < 2:416366/151‑1 (MQ=255) ttaaaaTTTAACAAAC‑CCGGCGG‑CGATATCAAGGCATGGCGTCTTGCTGTCCATCATTCCCACCAGGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag < 1:529048/148‑1 (MQ=255) ttcaaGTTAACAAAACCCGACGG‑CGAAATCAAGGCAGGGCGATTTCCTGTCAATCTGTTCCACAGGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag < 1:39004/148‑1 (MQ=255) aTCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGAt > 2:468832/1‑151 (MQ=255) aaaaac‑CCGAGGG‑GAATTTCAAGGATGGGCGATTTGCTGTCCATCATTTCCCCCAGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGAtt < 2:385136/149‑1 (MQ=255) cGACGG‑CGATATCAAGGCAGGGCGATTTGCTGTCCATCAGTCCCCCCACGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCg < 2:350087/151‑1 (MQ=255) cGG‑CGATAAAAGGGCATGCCTTCTTGCTGCCCATCATTCCCCCCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTc < 1:359798/151‑1 (MQ=255) atatCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTg > 2:420132/1‑149 (MQ=255) atatCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTg > 1:178744/1‑149 (MQ=255) tatCAGGGCAGGGCGATTTGCTGCCCATCAGTCCCCCCACGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTc < 2:50470/151‑1 (MQ=255) cATGGCGATTTGCTGCCCATCAGTCCCCCCAGGGA‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTcc < 2:71517/150‑1 (MQ=255) ttgttgTCCATCGTTCCCACCGCGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAc < 1:297672/151‑1 (MQ=255) ttGCTGTCCATCAGTCCCCCCGGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAc < 1:290022/151‑1 (MQ=255) ttGCGGTCCACCAGTCCCACCCGGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCGGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAc < 2:188810/151‑1 (MQ=255) gTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAg > 1:401585/1‑151 (MQ=255) aGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCa > 1:156620/1‑151 (MQ=255) gTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAg > 2:280317/1‑151 (MQ=255) | TTGATGATAGTACGTTCGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CCGACGG‑CGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAG > NC_000913/3578137‑3578435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |